Human EDDM3B Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13739-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

EDDM3B cDNA Clone Datasheet

EDDM3B cDNA Clone Product Information

Gene Bank Ref.ID:	NM_022360.4
cDNA Size:	444
Gene Synonym:	EP3B; HE3B; FAM12B; HE3-BETA; EDDM3B
Source:	Human

EDDM3B cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens epididymal protein 3B DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

EDDM3B cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

EDDM3B cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

EDDM3B cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCATCGT CTCTAAAGAT CTGGGGCACA CTCTTGGCCC TACTTTGCAT CCTATGCACA
61       CTGCTTGTAC AGAGCAAAGA AGTTTCTTGG AGAGAATTCA TGAAACAGCA CTACTTAAGT
121      CCAAGTCGAG AATTCAGAGA GTACAAATGT GATGTCCTCA TGAGAGAAAA TGAAGCTCTG
181      AAAGACAAGA GCTCTCACAT GTTTATCTAT ATCTCATGGT ACAAAATCGA GCATATATGC
241      ACTAGTGACA ACTGGATGGA TCGCTTCCGA AATGCATATG TATGGGTCCA GAATCCTCTC
301      AAAGTACTCA AGTGTCACCA GGAGAATTCC AAAAATAGCT ACACAGAGAG CAGGAGCTTC
361      AACTACATTG AATTCCATTG TAGCATGGAC GGGTATGTTG ATAGCATAGA AGACCTAAAG
421      ATGGTAGAAC CTATCGGCAA CTAG

Quality Control:cDNA sequencing results is available upon request.