|Datasheet||Specific References||Reviews||Related Products||Protocols|
|ORF Clone of Rattus norvegicus ectodysplasin-A receptor DNA.|
|Identical with the Gene Bank Ref. ID sequence except for the point mutation 816 C/T，819 C/A and 1134 T/C not causing the amino acid variation.|
|Whatman FTA elute card (Cat: WB120410) contains 5-10 μg of plasmid.|
|The Whatman FTA elute card can be stored at room temperature for three months under dry condition.|
The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.
The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.
|Rat EDAR Gene cDNA Clone (full-length ORF Clone), expression ready, FLAG-tagged||RG80228-G-F|
|Rat EDAR Gene cDNA Clone (full-length ORF Clone), expression ready, His-tagged||RG80228-G-H|
|Rat EDAR Gene cDNA Clone (full-length ORF Clone), expression ready, Myc-tagged||RG80228-G-M|
|Rat EDAR Gene cDNA Clone (full-length ORF Clone), expression ready, untagged||RG80228-G-N|
|Rat EDAR Gene cDNA Clone (full-length ORF Clone), expression ready, HA-tagged||RG80228-G-Y|
|Product name||Product name|
Tumor necrosis factor receptor superfamily member EDAR is a Single-pass type I membrane protein. Edar was expressed reiteratively in signaling centers regulating key steps in morphogenesis. activin signaling from mesenchyme induces the expression of the TNF receptor edar in the epithelial signaling centers, thus making them responsive to Wnt-induced ectodysplasin from the nearby ectoderm. This is the first demonstration of integration of the Wnt, activin, and TNF signaling pathways. Defects in EDAR are a cause of ectodermal dysplasia anhidrotic (EDA), also known ectodermal dysplasia hypohidrotic autosomal recessive (HED). Ectodermal dysplasia defines a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. EDA is characterized by sparse hair (atrichosis or hypotrichosis), abnormal or missing teeth and the inability to sweat due to the absence of sweat glands.