Rat EDAR Gene cDNA Clone / ORF Clone (Cytokine)

Catalog	Vector (Price)	Quantity	In Stock	Operation
RG80228-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

EDAR cDNA Clone Datasheet

EDAR cDNA Clone Product Information

Gene Bank Ref.ID:	NM_001191899.1
cDNA Size:	1347
Gene Synonym:	RGD1561714; Edar
Source:	Rat

EDAR cDNA Clone Description

cDNA Description:	ORF Clone of Rattus norvegicus ectodysplasin-A receptor DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref. ID sequence except for the point mutation 816 C/T，819 C/A and 1134 T/C not causing the amino acid variation.

EDAR cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

EDAR cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

EDAR cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCCCACG TCGGGGACTG CAAGTGGATG TCCTGGCTCC CAGTGCTGGT GGTGTCTCTG
61       ATGTGCTCAG CCAAGGCAGA GGACTCCAAC TGTGGCGAGA ATGAATACCA CAACCAAACT
121      ACCGGGCTGT GCCAGCAGTG TCCTCCATGC AGACCAGGGG AGGAGCCCTA CATGTCCTGT
181      GGTTACGGCA CCAAAGATGA GGACTATGGC TGCGTGCCCT GCCCTGCAGA GAAGTTCTCC
241      AAAGGAGGTT ATCAGATATG CAGGCGCCAC AAGGACTGTG AGGGCTTCTT CCGGGCCACC
301      GTACTGACAC CAGGAGACAT GGAAAATGAT GCTGAGTGTG GCCCGTGTCT GCCTGGCTAC
361      TACATGCTGG AAAACAGACC CAGGAACATC TACGGCATGG TCTGCTACTC CTGCCTCTTG
421      GCACCTCCCA ACACCAAGGA ATGTGTGGGA GCCACTTCCG GGGTTTCAGC TCACTCCTCC
481      AGCACTTCAG GCGGCAGCAC CTTGTCCCCC TTCCAGCATG CTCACAAAGA GCTCTCAAGT
541      CAAGGACACC TGGCCACCGC CCTGATTATT GCCATGTCTA CAATCTTCAT CATGGCCATA
601      GCCATTGTCC TCATCATCAT GTTCTATATC ATGAAGACTA AGCCTTCAGC TCCAGCCTGC
661      TGTAGCAGTC CCCCAGGAAA AAGTGCAGAA GCCCCAGCTA ACACACATGA GGAGAAAAAA
721      GAGGCCCCAG ACAGTGTGGT GACTTTCCCT GAGAATGGTG AATTCCAGAA GTTGACAGCA
781      ACACCCACAA AGACCCCAAA AAGTGAGAAC GATGCTTCAT CTGAGAATGA GCAGCTGCTG
841      AGTCGCAGTG TGGACAGTGA TGAAGAGCCA GCCCCGGACA AGCAGGGGTC CCCAGAGCTA
901      TGCCTGCTGT CGCTAGTTCA TCTGGCCAGG GAGAAGTCTG TAGCCAGTAA CAAGTCTGCT
961      GGGATCCAAA GCCGAAGGAA AAAGATATTG GATGTGTATG CGAACGTGTG TGGTGTTGTT
1021     GAAGGTCTCA GCCCCACGGA GCTGCCTTTT GATTGCCTTG AAAAGACAAG CAGAATGCTC
1081     AGTTCTACCT ACAACTCTGA GAAAGCAGTC GTGAAAACGT GGCGCCACCT TGCCGAGAGC
1141     TTTGGACTGA AGAGGGATGA GATTGGGGGC ATGACTGATG GCATGCAGCT CTTTGACCGC
1201     ATCAGCACTG CGGGCTACAG CATCCCAGAG CTGCTCACGA AGCTGGTACA GATCGAGAGG
1261     CTGGATGCTG TGGAGTCCTT GTGTGCAGAC ATATTGGAGT GGGCTGGGGT TGTACCCCCT
1321     GCCTCCCCAC CCCCAGCTGC ATCCTGA

Quality Control:cDNA sequencing results is available upon request.

EDAR related areas, pathways, and other information

Cytokine