Rat EDA2R Gene cDNA Clone / ORF Clone

EDA2R cDNA Clone Datasheet

EDA2R cDNA Clone Product Information

• Gene Bank Ref.ID: XM_231469.5
• cDNA Size: 897
• Gene Synonym: RGD1564025, Eda2r
• Source: Rat

EDA2R cDNA Clone Description

• cDNA Description: ORF Clone of Rattus norvegicus ectodysplasin A2 receptor DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

EDA2R cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

EDA2R cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

EDA2R cDNA Clone Sequence and Quality Control

Sequence:

1        ATGATGGATT GTCAAGAAAA TGAGTACCGG GACCAATGGG GGCGCTGTGT CACCTGCCAA
61       CAATGTGGCC CTGGACAGGA GCTCTCTAAG GATTGTGGTT ATGGAGAAGG TGGAGATGCG
121      TACTGCATAG CCTGCCCTCC TCGAAAGTAT AAAAGCACTT GGGGACATCA CAGATGTCAG
181      ACATGCATCA CTTGTGCTGT CATCAATCGG GTCCAGAAGG CCAACTGCAC AAATACCTCT
241      AATGCTATCT GTGGAGACTG TCTGCCCAGG TTCTACCGAA AGACACGCAT TGGGGGCCTG
301      CAGGACCAAG AATGCATCCC GTGTACAAAG CAGACTCCTT CTTCTGAGGT TCAGTGTACC
361      TTCCAGCTGA GCTTAGTGAA GGTAGACACA CACACTCTTC CTCCTAAGGA AGCCACACTT
421      GTCGCACTGG TAGGAAGTCT GCTAGTGGTC TTTGCCCTGG CCTTCCTTGG ACTCTTCTTC
481      CTTTATTGCA AGCAAATATT CAACAGACAT TGTCAGTGTG GAGGTTCATT GCAATATGAG
541      GCTGAAAAGG CAGTGCAGGA GGATTCTCTT TTAACCACAC CACCTGGCCA GGAGACCAGT
601      CCTGAGTTTC CAGCAAATGG GAGCATCTTT GAGATCAAGC CCCTTAACTC CATCCTGGAT
661      GATGACTGCA GCTCCACTCG CGGCTTCCCC ACTCAGGAAT CCTTCACCAT GGCTTCCTGC
721      GCCTCAGAGA GCCATTCTCA GTGGGTCCAT ACCCCCATTG AATGTACAGA GCTGGACCTG
781      CAAAAGTTTT CCAGCTCTGT CCCCTGTCCT GGAGCTGAAA CCTTGAGAGA GAACACAGCT
841      GAACACTCAG GAGACAGGCT GGACCTCTAT GTACCTTTTG AAGTTCCCAG CCTTTAA

 

Quality Control:cDNA sequencing results is available upon request.