ECM1 Antibody

ECM1 Antibody Datasheet

ECM1 Antibody Product Information

• Immunogen: Recombinant Mouse ECM1 protein (Catalog#50331-M08H)
• Antibody Type: Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
• Clone ID: 001
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Mouse ECM1 (rM ECM1; Catalog#50331-M08H; Q61508-1; Met 1-Glu 559).

ECM1 Antibody Usage Guide

• Specificity: Mouse ECM1
• Has cross-reactivity in ELISA with
  

  Human ECM1

• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse ECM1. The detection limit for Mouse ECM1 is approximately 0.039 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

ECM1 Antibody Background

Extracellular matrix protein-1 (ECM1) is a secreted glycoprotein important in connective tissue organization. Three splice variants have been identified: ECM1a (540 aa) is most widely expressed, with highest expression in the placenta and heart; ECM1b (415 aa) is differentiation-dependent expressed and found only in tonsil and associated with suprabasal keratinocytes; ECM1c (559 aa) accounts for approximately 15% of skin ECM1. ECM1, although not tumor specific, is significantly elevated in many malignant epithelial tumors and is suggested as a possible trigger for angiogenesis, tumor progression and malignancies. ECM1 also has been shown to regulate endochondral bone formation, skeletal development and tissue remodeling. Two disorders are associated with diminished function of ECM1, referred to as lipoid proteinosis which is a autosomal recessive disorder typified by generalized thickening of skin, mucosae and certain viscera, and an autoimmune disease lichen sclerosis. The pathological phenotypes support a role for ECM1 as a "biological glue" in the dermis, and exerts actions in skin adhesion, epidermal differentiation, wound healing, and basement membrane physiology. Recent studies have revealed that ECM1 is implicated in the common inflammatory bowel diseases, ulcerative colitis and Crohn's disease.

References

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6. Fisher, S.A. et al., 2008, Nat. Genet. 40: 710-712.