Mouse EBI3 Gene cDNA Clone / ORF Clone

EBI3 cDNA Clone Datasheet

EBI3 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_015766.2
• cDNA Size: 687
• Gene Synonym: IL27B
• Source: Mouse

EBI3 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus Epstein-Barr virus induced 3 DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 245 C/A resulting in the amino acid ser substitution by tyr.

EBI3 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

EBI3 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

EBI3 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGTACAAGC TGCTCTTCCT GTCACTTGCC CTCTGGGCCA GCCGCTCCCC TGGTTACACT
61       GAAACAGCTC TCGTGGCTCT AAGCCAGCCC AGAGTGCAAT GCCATGCTTC TCGGTATCCC
121      GTGGCCGTGG ACTGCTCCTG GACTCCTCTC CAGGCTCCCA ACTCCACCAG ATCCACGTCC
181      TTCATTGCCA CTTACAGGCT CGGTGTGGCC ACCCAGCAGC AGAGCCAGCC CTGCCTACAA
241      CGGAGCCCCC AGGCCTCCCG ATGCACCATC CCCGACGTGC ACCTGTTCTC CACGGTGCCC
301      TACATGCTAA ATGTCACTGC AGTGCACCCA GGCGGCGCCA GCAGCAGCCT CCTAGCCTTT
361      GTGGCTGAGC GAATCATCAA GCCGGACCCT CCGGAAGGCG TGCGCCTGCG CACAGCGGGA
421      CAGCGCCTGC AGGTGCTCTG GCATCCCCCT GCTTCCTGGC CCTTCCCGGA CATCTTCTCT
481      CTCAAGTACC GACTCCGCTA CCGGCGCCGA GGAGCCTCTC ACTTCCGCCA GGTGGGACCC
541      ATTGAAGCCA CGACTTTCAC CCTCAGGAAC TCGAAACCCC ATGCCAAGTA TTGCATCCAG
601      GTGTCAGCTC AGGACCTCAC AGATTATGGG AAACCAAGTG ACTGGAGCCT CCCTGGGCAA
661      GTAGAAAGTG CACCCCATAA GCCCTGA

 

Quality Control:cDNA sequencing results is available upon request.