( We provide with EBI3 qPCR primers for gene expression analysis, HP100191 )
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constiutive ,Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.
A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis.
The peptide sequence of the FLAG-tag from the N-terminus to the C-terminus is: DYKDDDDK (1012 Da). It can be used in conjunction with other affinity tags, for example a polyhistidine tag (His-tag), HA-tag or myc-tag. It can be fused to the C-terminus or the N-terminus of a protein. Some commercially available antibodies (e.g., M1/4E11) recognize the epitope only when it is present at the N-terminus. However, other available antibodies (e.g., M2) are position-insensitive.
|Human EBI3 / IL27b Gene ORF cDNA clone expression plasmid, C-GFPSpark tag||HG10117-ACG|
|Human EBI3 / IL27b Gene ORF cDNA clone expression plasmid, C-OFPSpark tag||HG10117-ACR|
|Human EBI3 / IL27b Gene ORF cDNA clone expression plasmid, C-Flag tag||HG10117-CF|
|Human EBI3 / IL27b Gene ORF cDNA clone expression plasmid, C-His tag||HG10117-CH|
|Human EBI3 / IL27b Gene ORF cDNA clone expression plasmid, C-Myc tag||HG10117-CM|
|Human EBI3 / IL27b Gene ORF cDNA clone expression plasmid, C-HA tag||HG10117-CY|
|Human EBI3 / IL27b Gene ORF cDNA clone in cloning vector||HG10117-M|
|Human EBI3 / IL27b Gene ORF cDNA clone expression plasmid||HG10117-M-N|
|Human EBI3 / IL27b Gene ORF cDNA clone expression plasmid, N-Flag tag||HG10117-NF|
|Human EBI3 / IL27b Gene ORF cDNA clone expression plasmid, N-His tag||HG10117-NH|
|Human EBI3 / IL27b Gene ORF cDNA clone expression plasmid, N-Myc tag||HG10117-NM|
|Human EBI3 / IL27b Gene ORF cDNA clone expression plasmid, N-HA tag||HG10117-NY|
|Human EBI3 / IL27b Gene ORF cDNA clone expression plasmid||HG10117-UT|
|Learn more about expression Vectors|
The novel Ebi3-IL-12alpha heterodimeric cytokine has been designated interleukin-35 (IL-35), is a member IL12 family cytokine produced by regulatory T cells (Treg), but not by resting or activated effector T cells (Teff). IL-35 is a heterodimeric protein composed of IL-12α (P35) and IL-27β chains, which are encoded by two separate genes called IL12A and EBI3 (Epstein-Barr-virus-induced gene 3) respectively. Ectopic expression of IL-35 confers regulatory activity on naive T cells, whereas recombinant IL-35 suppresses T-cell proliferation. It identify IL-35 as a novel inhibitory cytokine that may be specifically produced by T(reg) cells and is required for maximal suppressive activity. IL-35 has biological activity and able to expand CD4+CD25+ Treg cells, suppress the proliferation of CD4+CD25- effector cells and inhibit Th17 cell polarization. IL-35 has been shown to be constitutively expressed by regulatory T (Treg) cells CD4(+)CD25(+)Foxp3(+) and suggested to contribute to their suppressive activity. IL-35 is a crucial mediator which provokes CD4+CD25+ T cell proliferation and IL-10 generation, another well-known anti-inflammatory cytokine, along with TGFbeta cytokine. IL-35 is a cytokine can downregulate Th17 cell development and inhibit autoimmune inflammation. It inhibited the differentiation of Th17 cells in vitro. In vivo, IL-35 effectively attenuated established collagen-induced arthritis in mice, with concomitant suppression of IL-17 production but enhanced IFN-gamma synthesis. Thus, IL-35 is a novel anti-inflammatory cytokine suppressing the immune response through the expansion of regulatory T cells and suppression of Th17 cell development.