Human E2F3 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13856-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

E2F3 cDNA Clone Datasheet

E2F3 cDNA Clone Product Information

Gene Bank Ref.ID:	BC016847
cDNA Size:	402
Gene Synonym:	RP1-177P22.2, DKFZp686C18211, E2F-3, KIAA0075, MGC104598,E2F3
Source:	Human

E2F3 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens E2F transcription factor 3 DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

E2F3 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

E2F3 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

E2F3 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCAAAGTG GTGGTGGGGT CAAGACAGAT GACACCAGCA CTTTAAACTC TTTGTGTGGG
61       TATGCGTGGG TGTATGTTTG GGAAGAAAAA CAAAGGTGCA GACTATCTTC CTTTTTTTCT
121      TCTTCAGCCT CCATCCCTGG CCTCCTCCCC TCACACACAC TGGACTTGGT ACAAAATGTC
181      GGTGTGGTCC TAGATGAAGC ATTGGGGTGG GGGAGGGAGA GGGAGCTTTG TGTTAAGTGC
241      CTACTGGAAA TGCACTGTGG GGTTTTTTCC TGTATGGGAA ACCATTTATG CCAAGCTTTT
301      CCCCATTTCC CATATTTATC TCATCTGGTT AGCTGCCTCT GCTTCCAGCT TTGTGTAATT
361      CTCTTTGCCA GCTGCACAAA GCTGATTTTT TCCAAAGTCT AA

Quality Control:cDNA sequencing results is available upon request.