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>Human Cell Expressed
>Rat E-Cadherin / CDH1 / E-cad / CD324 Protein (Fc Tag)
|Catalog||Size (Price)||Quantity||In Stock||Operation|
Cadherin 1 Protein Datasheet
E-cadherin / CDH1 / Cadherin-1 Protein Price Inquiry ( Available Sizes )
E-cadherin / CDH1 / Cadherin-1 Protein Product Information
A DNA sequence encoding the rat CDH1 (Q9R0T4) (Met1-Ala713) was expressed with the Fc region of human IgG1 at the C-terminus.
|Expression Host:||Human Cells|
E-cadherin / CDH1 / Cadherin-1 Protein QC Testing
|Purity:||> 75 % as determined by SDS-PAGE||SDS-PAGE:
E-cadherin / CDH1 / Cadherin-1 protein
|Endotoxin:||< 1.0 EU per μg of the protein as determined by the LAL method|
|Stability:||Samples are stable for up to twelve months from date of receipt at -70℃|
|Predicted N terminal:||Gln 24|
The recombinant rat CDH1/Fc is a disulfide-linked homodimer. The reduced monomer comprises 931 amino acids and has a predicted molecular mass of 103.8 kDa. The apparent molecular mass of the protein is approximately 116 kDa in SDS-PAGE under reducing conditions.
|Formulation:||Lyophilized from sterile PBS, pH 7.4.
E-cadherin / CDH1 / Cadherin-1 Protein Usage Guide
|Storage:||Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.|
|Reconstitution:||A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.|
E-cadherin / CDH1 / Cadherin-1 Protein Related Products & Topics
|Molecule||Species||Description //For Detailed Info. and Price------CLICK!||Cat. No|
|E-Cadherin/CDH1/CD324||Human||E-Cadherin / CDH1 / /CD324 Protein, Recombinant||10204-H08H|
|E-Cadherin/CDH1/CD324||Mouse||E-Cadherin / CDH1 / CD324 Protein, Recombinant||50671-M08H|
|E-Cadherin/CDH1/CD324||Rat||E-cadherin / CDH1 / Cadherin-1 Protein, Recombinant||80274-R02H|
|Molecule||Application||Description //For Detailed Info. and Price------CLICK!||Cat. No|
|WB, ELISA||Mouse Monoclonal Antibody||10204-MM07|
|ELISA||Mouse Monoclonal Antibody||10204-MM12|
|WB, ELISA||Rabbit Polyclonal Antibody||10204-RP01|
|WB, ELISA||Rabbit Polyclonal Antibody (Antigen Affinity Purified)||10204-RP02|
E-cadherin / CDH1 / Cadherin-1 Protein Description
Cadherins are calcium-dependent cell adhesion proteins which preferentially interact with themselves in a homophilic manner in connecting cells, and thus may contribute to the sorting of heterogeneous cell type. E-cadherin (E-Cad), also known as CDH1 and CD324, is the classical cadherin molecule and contains five cadherin domains of ~110 aa each in the extracellular domain. E-Cad is expressed in the non-neural epithelial tissues as disulfide-linked homodimer, and plays a key role in the organization and integrity of most epithelial tissues, cell differentiation and tissue development. CDH1 interacts directly, via the cytoplasmic domain, with CTNNB1 or JUP to form the PSEN1/cadherin/catenin adhesion complex which connects to the actin skeleton through the actin binding of alpha-catenin. During apoptosis or with calcium influx, E-Cad is cleaved by the metalloproteinase to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. E-Cad has been identified as a potent invasive suppressor, as downregulation of E-cadherin expression is involved in dysfunction of the cell-cell adhesion system, and often correlates with strong invasive potential and poor prognosis of human carcinomas (gastric, breast, ovary, endometrium and thyroid).
- Berx G. et al., 1995, Genomics 26: 281-9.
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