E-Selectin / CD62e / SELE Antibody (Antigen Affinity Purified)

E-Selectin / CD62e / SELE Antibody ( Antigen Affinity Purified ) Datasheet

E-Selectin / CD62e / SELE Antibody Product Information

• Immunogen: Recombinant Rat E-Selectin / CD62e / SELE protein (Catalog#80033-R08H)
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, recombinant Rat E-Selectin / CD62e / SELE (rR E-Selectin / CD62e / SELE; Catalog#80033-R08H; P98105-1; Met 1-Pro 494). E-Selectin / CD62e / SELE specific IgG was purified by Rat E-Selectin / CD62e / SELE affinity chromatography.

E-Selectin / CD62e / SELE Antibody Usage Guide

• Specificity: Rat E-Selectin / CD62e / SELE
• Western blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Rat SELE in WB. Using a DAB detection system, the detection limit for Rat SELE is approximately 0.5 ng/lane under non-reducing conditions and 1 ng/lane under reducing conditions.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Rat SELE. The detection limit for Rat SELE is approximately 0.00245 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

E-Selectin / CD62e / SELE Antibody Background

E-selectin, also known as endothelial leukocyte adhesion molecule-1 (ELAM-1) and CD62E, is an inducible adhesion molecule that is expressed at relatively high density on the surface of cultured endothelial cells that have been activated by proinflammatory cytokines, such as IL-1β, TNF-α, or bacterial endotoxin. E-Selectin/CD62e exhibits a complex mosaic structure consisting of a large extracellular region comprised of a lectin domain, an EGF-like domain, and a short consensus repeat (SCR) domain, followed by a transmembrane region and a relatively short (32 aa) cytoplasmic tail. As a member of the LEC-CAM or selectin family, E-Selectin/CD62e recognises and binds to sialylated carbohydrates including members of the Lewis X and Lewis A families found on monocytes, granulocytes, and T-lymphocytes. E-Selectin/CD62e supports rolling and stable arrest of leukocytes on activated vascular endothelium, and furthermore, it was indicated that it can also transduce an activating stimulus via the MAPK cascade into the endothelial cell during leukocyte adhesion. In addition, this adhesion molecule appears to be involved in the pathogenesis of atherosclerosis.

References

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