E-Cadherin Matched ELISA Antibody Pair Set,Human

Cat: SEK10204

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E-Cadherin Matched ELISA Antibody Pair Set,Human General Information

Product name
E-Cadherin Matched ELISA Antibody Pair Set,Human
Assay type
Solid Phase Sandwich ELISA
Specificity
Quantitative determination of Human E-Cadherin
Assay range
46.88-3000
Materials provided
1. Capture Antibody 0.4 mg/mL of mouse anti-Human E-Cadherin / CDH1 / E-cad / CD324 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating. (Catalog: # 10204-MM04)
2. Detection Antibody 0.2 mg/mL of rabbit anti-Human E-Cadherin / CDH1 / E-cad / CD324 polyclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use.
3. Standard Each vial contains 57 ng of recombinant Human E-Cadherin / CDH1 / E-cad / CD324. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 3000 pg/mL is recommended.
Product overview
This E-Cadherin Matched ELISA Antibody Pair Set,Human is a solid phase sandwich ELISA for quantitative determination of Human E-Cadherin . It contains Human E-Cadherin capture antibody, Human E-Cadherin detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human E-Cadherin protein. This Pair Set is at affordable price for researchers.
Shipping
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
Storage
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.

E-Cadherin Matched ELISA Antibody Pair Set,Human Images

Human E-Cadherin/CDH1/E-cad/CD324 ELISA Kit Standard Curve 7057

E-Cadherin Matched ELISA Antibody Pair Set,Human Alternative Names

Arc-1 Matched ELISA Antibody Pair Set, Human;CD324 Matched ELISA Antibody Pair Set, Human;CDH1 Matched ELISA Antibody Pair Set, Human;CDHE Matched ELISA Antibody Pair Set, Human;E-cad Matched ELISA Antibody Pair Set, Human;E-Cadherin Matched ELISA Antibody Pair Set, Human;ECAD Matched ELISA Antibody Pair Set, Human;LCAM Matched ELISA Antibody Pair Set, Human;UVO Matched ELISA Antibody Pair Set, Human

E-Cadherin Background Information

Cadherins are calcium-dependent cell adhesion proteins which preferentially interact with themselves in a homophilic manner in connecting cells, and thus may contribute to the sorting of heterogeneous cell type. E-cadherin (E-Cad), also known as CDH1 and CD324, is a calcium-dependent cell adhesion molecule the intact function of which is crucial for the establishment and maintenance of epithelial tissue polarity and structural integrity. Mutations in CDH1 occur in diffuse type gastric cancer, lobular breast cancer, and endometrial cancer. In human cancers, partial or complete loss of E-cadherin expression correlates with malignancy. During apoptosis or with calcium influx, E-Cad is cleaved by the metalloproteinase to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. E-Cad has been identified as a potent invasive suppressor, as downregulation of E-cadherin expression is involved in dysfunction of the cell-cell adhesion system, and often correlates with strong invasive potential and poor prognosis of human carcinomas.
Full Name
cadherin 1, type 1, E-cadherin (epithelial)
References
  • Wang HD, et al. (2004) CDH1 germline mutation in hereditary gastric carcinoma. World J Gastroenterol. 10(21): 3088-93.
  • Masterson J, et al. (2007) Posttranslational truncation of E-cadherin and significance for tumour progression. Cells Tissues Organs. 185(1-3): 175-9.
  • Mrgineanu E, et al. (2008) Correlation between E-cadherin abnormal expressions in different types of cancer and the process of metastasis. Rev Med Chir Soc Med Nat Iasi. 112(2): 432-6.
  • Product Description Assay Range (pg/ml) Sample Type Catalog# (PDF)
    E-Cadherin ELISA Kit, Human Serum KIT10204
    info info
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