Anti-E-Cadherin Antibody

Cat: 50671-RP02
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Anti-E-Cadherin Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-E-Cadherin Antibody
Validated applications
WB,ELISA,IHC-P,IP
Species reactivity
Reacts with: Mouse
Specificity
Mouse E-Cadherin
Immunogen
Recombinant Mouse E-Cadherin / CDH1 protein (Catalog#50671-M08H)
Preparation
Produced in rabbits immunized with purified, recombinant Mouse E-Cadherin / CDH1 (rM E-Cadherin / CDH1; Catalog#50671-M08H; P09803; Met 1-Val 709). E-Cadherin / CDH1 specific IgG was purified by Mouse E-Cadherin / CDH1 affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-E-Cadherin Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:2000  
ELISA 1:5000-1:10000  
IHC-P 1:500-1:2000  
IP 1-4 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-E-Cadherin Antibody (Rabbit Polyclonal antibody) Images
Mouse E-Cadherin/CDH1/E-cad/CD324 Immunohistochemistry(IHC) 3335
Immunochemical staining of mouse CDH1 in mouse stomach with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections). The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).
Mouse E-Cadherin/CDH1/E-cad/CD324 Immunohistochemistry(IHC) 3337
Immunochemical staining of mouse CDH1 in mouse liver with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections). The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).
Mouse E-Cadherin/CDH1/E-cad/CD324 Immunoprecipitation(IP) 6093

Mouse CDH1 was immunoprecipitated using:

Lane A:0.5 mg MCF-7 Whole Cell Lysate

2 µL anti-Mouse CDH1 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-Mouse CDH1 rabbit polyclonal antibody,at 1:200 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 130 kDa

Observed band size: 130 kDa

Mouse E-Cadherin/CDH1/E-cad/CD324 Western blot (WB) 13696

Anti-CDH1 rabbit polyclonal antibody at 1:500 dilution

Lane A: A431 Whole Cell Lysate

Lane B: MCF7 Whole Cell Lysate

Lane C: MDA-MB-468 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:97 kDa

Observed band size:150 kDa

Anti-E-Cadherin Antibody Alternative Names
Anti-AA960649 Antibody;Anti-ARC-1 Antibody;Anti-E-cad Antibody;Anti-Ecad Antibody;Anti-L-CAM Antibody;Anti-Um Antibody;Anti-UVO Antibody
E-Cadherin Background Information

Cadherins are calcium-dependent cell adhesion proteins which preferentially interact with themselves in a homophilic manner in connecting cells, and thus may contribute to the sorting of heterogeneous cell type. E-cadherin (E-Cad), also known as CDH1 and CD324, is a calcium-dependent cell adhesion molecule the intact function of which is crucial for the establishment and maintenance of epithelial tissue polarity and structural integrity. Mutations in CDH1 occur in diffuse type gastric cancer, lobular breast cancer, and endometrial cancer. In human cancers, partial or complete loss of E-cadherin expression correlates with malignancy. During apoptosis or with calcium influx, E-Cad is cleaved by the metalloproteinase to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. E-Cad has been identified as a potent invasive suppressor, as downregulation of E-cadherin expression is involved in dysfunction of the cell-cell adhesion system, and often correlates with strong invasive potential and poor prognosis of human carcinomas.

Full Name
cadherin 1, type 1, E-cadherin (epithelial)
References
  • Wang HD, et al. (2004) CDH1 germline mutation in hereditary gastric carcinoma. World J Gastroenterol. 10(21): 3088-93.
  • Masterson J, et al. (2007) Posttranslational truncation of E-cadherin and significance for tumour progression. Cells Tissues Organs. 185(1-3): 175-9.
  • Mrgineanu E, et al. (2008) Correlation between E-cadherin abnormal expressions in different types of cancer and the process of metastasis. Rev Med Chir Soc Med Nat Iasi. 112(2): 432-6.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-E-Cadherin Antibody Mouse Monoclonal ELISA 80274-MM04
    Anti-E-Cadherin Antibody Rabbit Polyclonal WB,ELISA,ICC/IF,IF 80274-T50
    Anti-E-Cadherin Antibody Rabbit Polyclonal ELISA 80274-RP01
    Anti-E-Cadherin Antibody Rabbit Polyclonal WB,ELISA,IHC-P,IP 50671-T60
    Anti-E-Cadherin Antibody Rabbit Polyclonal ELISA,IHC-P 50671-RP01
    Anti-E-Cadherin Antibody Rabbit Monoclonal WB,IP 50671-R117
    Anti-E-Cadherin Antibody Rabbit Monoclonal ELISA 10204-R055
    Anti-E-Cadherin Antibody Mouse Monoclonal ELISA(Cap) 10204-MM04
    Anti-E-Cadherin Antibody Rabbit Polyclonal WB,ELISA,IHC-P,ICC/IF,IF 10204-T58
    Anti-E-Cadherin Antibody Rabbit Polyclonal ELISA,IHC-P,ICC/IF,IF 10204-T26
    Anti-E-Cadherin Antibody Mouse Monoclonal ELISA 10204-MM07
    Anti-E-Cadherin Antibody Rabbit Polyclonal WB,ELISA,IHC-P,IHC-Fr 10204-RP02
    Anti-E-Cadherin Antibody (APC) Mouse Monoclonal FCM 10204-MM08-A
    Anti-E-Cadherin Antibody Mouse Monoclonal FCM 10204-MM08
    Anti-E-Cadherin Antibody (FITC) Mouse Monoclonal FCM,ICC/IF,IF 10204-MM08-F
    Anti-E-Cadherin Antibody Rabbit Polyclonal ELISA 10204-RP01
    Anti-E-Cadherin Antibody Rabbit Monoclonal IHC-P,ICC/IF,IF 10204-R032
    Anti-E-Cadherin Antibody Mouse Monoclonal ELISA 10204-MM12
    Anti-E-Cadherin Antibody (PE) Mouse Monoclonal FCM,ICC/IF,IF 10204-MM08-P
    Anti-E-Cadherin Antibody Rabbit Monoclonal ELISA 10204-R301
    Anti-E-Cadherin Antibody Rabbit Monoclonal WB,IP 10204-R056
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