E-Cadherin / CDH1 Antibody (Antigen Affinity Purified)

E-Cadherin / CDH1 Antibody ( Antigen Affinity Purified ) Datasheet

E-Cadherin / CDH1 Antibody Product Information

• Immunogen: Recombinant Mouse E-Cadherin / CDH1 protein (Catalog#50671-M08H)
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, recombinant Mouse E-Cadherin / CDH1 (rM E-Cadherin / CDH1; Catalog#50671-M08H; P09803; Met 1-Val 709). E-Cadherin / CDH1 specific IgG was purified by Mouse E-Cadherin / CDH1 affinity chromatography.

E-Cadherin / CDH1 Antibody Usage Guide

• Specificity: Mouse E-Cadherin / CDH1
• Immunochemistry: IHC-P: 0.1-2 μg/mL

  

  Immunochemical staining of mouse CDH1 in mouse liver with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections). The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).

  

  Immunochemical staining of mouse CDH1 in mouse stomach with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections). The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).

• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse CDH1. The detection limit for Mouse CDH1 is approximately 0.00245 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

E-Cadherin / CDH1 Antibody Background

Cadherins are calcium-dependent cell adhesion proteins which preferentially interact with themselves in a homophilic manner in connecting cells, and thus may contribute to the sorting of heterogeneous cell type. E-cadherin (E-Cad), also known as CDH1 and CD324, is the classical cadherin molecule and contains five cadherin domains of ~110 aa each in the extracellular domain. E-Cad is expressed in the non-neural epithelial tissues as disulfide-linked homodimer, and plays a key role in the organization and integrity of most epithelial tissues, cell differentiation and tissue development. CDH1 interacts directly, via the cytoplasmic domain, with CTNNB1 or JUP to form the PSEN1/cadherin/catenin adhesion complex which connects to the actin skeleton through the actin binding of alpha-catenin. During apoptosis or with calcium influx, E-Cad is cleaved by the metalloproteinase to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. E-Cad has been identified as a potent invasive suppressor, as downregulation of E-cadherin expression is involved in dysfunction of the cell-cell adhesion system, and often correlates with strong invasive potential and poor prognosis of human carcinomas (gastric, breast, ovary, endometrium and thyroid).

References

1. Berx G. et al., 1995, Genomics 26: 281-289.
2. Yoshiura K. et al., 1995, Proc. Natl. Acad. Sci. U.S.A. 92: 7416-7419.
3. Becker K.-F. et al., 1994, Cancer Res. 54: 3845-3852.
4. Marambaud P. et al., 2002, EMBO J. 21: 1948-1956.
5. Steinhusen U. et al., 2001, J. Biol. Chem. 276: 4972-4980.