Anti-E-Cadherin Antibody

Cat: 10204-RP02
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Anti-E-Cadherin Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-E-Cadherin Antibody
Validated applications
WB,ELISA,IHC-P,IHC-Fr
Species reactivity
Reacts with: Human
Not React with: Rat
Specificity
Human E-Cadherin
Immunogen
Recombinant Human CDH1 protein (Catalog#10204-H08H)
Preparation
Produced in rabbits immunized with purified, recombinant Human Cadherin-1 / CDH1 (rhCadherin-1; Catalog#10204-H08H; NP_004351.1; Met 1-Ile 707). Cadherin-1 specific IgG was purified by human Cadherin-1 affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-E-Cadherin Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:1000  
ELISA 1:5000-1:10000  
IHC-P 1:2000-1:10000  
IHC-Fr 1:500-1:2500  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-E-Cadherin Antibody (Rabbit Polyclonal antibody) Images
Human E-Cadherin/CDH1/E-cad/CD324 Immunohistochemistry(IHC) 2940
Immunofluorescence staining of human E-cadherin in human skin with Rabbit Polyclonal Antibody at 1:1000 dilution, frozen sections. An DyLight488 goat anti-rabbit antibody was used as the secondary antibody. The image showing membrane staining of squamous epitheliu
Human E-Cadherin/CDH1/E-cad/CD324 Immunohistochemistry(IHC) 4264
Immunochemical staining of human E-cadherin in liver with Rabbit Polyclonal Antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections. Positive staining was localized to membrane of hepatocytes.
Human E-Cadherin/CDH1/E-cad/CD324 Immunohistochemistry(IHC) 4265
Immunochemical staining of human E-cadherin in human skin with Rabbit Polyclonal Antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections. Positive staining was localized to membrane of epithelial cells. The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein.
Human E-Cadherin/CDH1/E-cad/CD324 Immunohistochemistry(IHC) 4268
Immunochemical staining of human E-cadherin in human gastric cancer with Rabbit Polyclonal Antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections. Positive staining was localized to membrane of epithelial cells.
Human E-Cadherin/CDH1/E-cad/CD324 Immunohistochemistry(IHC) 4272
Immunochemical staining of human E-cadherin in human colon carcinoma with Rabbit Polyclonal Antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections. Positive staining was localized to membrane of epithelial cells.
Human E-Cadherin/CDH1/E-cad/CD324 Western blot (WB) 13143

Anti-E-cad rabbit polyclonal antibody at 1:500 dilution

Lane A: HepG2 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:97 kDa

Observed band size:170 kDa

Anti-E-Cadherin Antibody Alternative Names
Anti-Arc-1 Antibody;Anti-CD324 Antibody;Anti-CDH1 Antibody;Anti-CDHE Antibody;Anti-E-cad Antibody;Anti-E-Cadherin Antibody;Anti-ECAD Antibody;Anti-LCAM Antibody;Anti-UVO Antibody
E-Cadherin Background Information

Cadherins are calcium-dependent cell adhesion proteins which preferentially interact with themselves in a homophilic manner in connecting cells, and thus may contribute to the sorting of heterogeneous cell type. E-cadherin (E-Cad), also known as CDH1 and CD324, is a calcium-dependent cell adhesion molecule the intact function of which is crucial for the establishment and maintenance of epithelial tissue polarity and structural integrity. Mutations in CDH1 occur in diffuse type gastric cancer, lobular breast cancer, and endometrial cancer. In human cancers, partial or complete loss of E-cadherin expression correlates with malignancy. During apoptosis or with calcium influx, E-Cad is cleaved by the metalloproteinase to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. E-Cad has been identified as a potent invasive suppressor, as downregulation of E-cadherin expression is involved in dysfunction of the cell-cell adhesion system, and often correlates with strong invasive potential and poor prognosis of human carcinomas.

Full Name
cadherin 1, type 1, E-cadherin (epithelial)
References
  • Wang HD, et al. (2004) CDH1 germline mutation in hereditary gastric carcinoma. World J Gastroenterol. 10(21): 3088-93.
  • Masterson J, et al. (2007) Posttranslational truncation of E-cadherin and significance for tumour progression. Cells Tissues Organs. 185(1-3): 175-9.
  • Mrgineanu E, et al. (2008) Correlation between E-cadherin abnormal expressions in different types of cancer and the process of metastasis. Rev Med Chir Soc Med Nat Iasi. 112(2): 432-6.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-E-Cadherin Antibody Mouse Monoclonal ELISA 80274-MM04
    Anti-E-Cadherin Antibody Rabbit Polyclonal WB,ELISA,ICC/IF,IF 80274-T50
    Anti-E-Cadherin Antibody Rabbit Polyclonal ELISA 80274-RP01
    Anti-E-Cadherin Antibody Rabbit Polyclonal WB,ELISA,IHC-P,IP 50671-T60
    Anti-E-Cadherin Antibody Rabbit Polyclonal ELISA,IHC-P 50671-RP01
    Anti-E-Cadherin Antibody Rabbit Monoclonal WB,IP 50671-R117
    Anti-E-Cadherin Antibody Rabbit Polyclonal WB,ELISA,IHC-P,IP 50671-RP02
    Anti-E-Cadherin Antibody Rabbit Monoclonal ELISA 10204-R055
    Anti-E-Cadherin Antibody Mouse Monoclonal ELISA(Cap) 10204-MM04
    Anti-E-Cadherin Antibody Rabbit Polyclonal WB,ELISA,IHC-P,ICC/IF,IF 10204-T58
    Anti-E-Cadherin Antibody Rabbit Polyclonal ELISA,IHC-P,ICC/IF,IF 10204-T26
    Anti-E-Cadherin Antibody Mouse Monoclonal ELISA 10204-MM07
    Anti-E-Cadherin Antibody (APC) Mouse Monoclonal FCM 10204-MM08-A
    Anti-E-Cadherin Antibody Mouse Monoclonal FCM 10204-MM08
    Anti-E-Cadherin Antibody (FITC) Mouse Monoclonal FCM,ICC/IF,IF 10204-MM08-F
    Anti-E-Cadherin Antibody Rabbit Polyclonal ELISA 10204-RP01
    Anti-E-Cadherin Antibody Rabbit Monoclonal IHC-P,ICC/IF,IF 10204-R032
    Anti-E-Cadherin Antibody Mouse Monoclonal ELISA 10204-MM12
    Anti-E-Cadherin Antibody (PE) Mouse Monoclonal FCM,ICC/IF,IF 10204-MM08-P
    Anti-E-Cadherin Antibody Rabbit Monoclonal ELISA 10204-R301
    Anti-E-Cadherin Antibody Rabbit Monoclonal WB,IP 10204-R056
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