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E-Cadherin / CDH1 / E-cad / CD324 Antibody, Rabbit PAb, Antigen Affinity Purified

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Human CDH1 Antibody Product Information
Immunogen:Recombinant Human CDH1 protein (Catalog#10204-H08H)
Clone ID:
Ig Type:Rabbit IgG
Concentration:
Endotoxin:
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:Produced in rabbits immunized with purified, recombinant Human Cadherin-1 / CDH1 (rhCadherin-1; Catalog#10204-H08H; NP_004351.1; Met 1-Ile 707). Cadherin-1 specific IgG was purified by human Cadherin-1 affinity chromatography.
Human CDH1 Antibody Usage Guide
Specificity:Human Cadherin-1 / CDH1 / CD324
Application:WB, ELISA, IHC-P

WB: 10-20 μg/mL

ELISA: 0.5-1.0 μg/mL

This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect Human Cadherin-1. The detection limit for Human Cadherin-1 is 0.00245 ng/well .

IHC-P: 0.5-2 μg/mL

IHC-Fr: 5-15 μg/mL

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Human CDH1 Antibody WB Application Image
E-Cadherin / CDH1 / E-cad / CD324 Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
Human CDH1 Antibody IHC Application Image
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Caption:
Immunochemical staining of human E-cadherin in human colon carcinoma with Rabbit Polyclonal Antibody (1 µg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to membrane of epithelial cells.
E-Cadherin / CDH1 / E-cad / CD324 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
[Click to enlarge image]
Caption:
Immunochemical staining of human E-cadherin in human gastric cancer with Rabbit Polyclonal Antibody (1 µg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to membrane of epithelial cells.
E-Cadherin / CDH1 / E-cad / CD324 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
[Click to enlarge image]
Caption:
Immunochemical staining of human E-cadherin in liver with Rabbit Polyclonal Antibody (1 µg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to membrane of hepatocytes.
E-Cadherin / CDH1 / E-cad / CD324 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
[Click to enlarge image]
Caption:
Immunochemical staining of human E-cadherin in human skin with Rabbit Polyclonal Antibody (1 µg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to membrane of epithelial cells. The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).
E-Cadherin / CDH1 / E-cad / CD324 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
[Click to enlarge image]
Caption:
Immunofluorescence staining of human E-cadherin in human skin with Rabbit Polyclonal Antibody (5 µg/mL, frozen sections). An DyLight488 goat anti-rabbit antibody was used as the secondary antibody. The image showing membrane staining of squamous epitheliu
E-Cadherin / CDH1 / E-cad / CD324 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunofluorescence
Other CDH1 Antibody Products
E-Cadherin/CDH1/E-cad/CD324 Background

Cadherins are calcium-dependent cell adhesion proteins which preferentially interact with themselves in a homophilic manner in connecting cells, and thus may contribute to the sorting of heterogeneous cell type. E-cadherin (E-Cad), also known as CDH1 and CD324, is a calcium-dependent cell adhesion molecule the intact function of which is crucial for the establishment and maintenance of epithelial tissue polarity and structural integrity. Mutations in CDH1 occur in diffuse type gastric cancer, lobular breast cancer, and endometrial cancer. In human cancers, partial or complete loss of E-cadherin expression correlates with malignancy. During apoptosis or with calcium influx, E-Cad is cleaved by the metalloproteinase to produce fragments of about 38 kDa (E-CAD/CTF1), 33 kDa (E-CAD/CTF2) and 29 kDa (E-CAD/CTF3), respectively. E-Cad has been identified as a potent invasive suppressor, as downregulation of E-cadherin expression is involved in dysfunction of the cell-cell adhesion system, and often correlates with strong invasive potential and poor prognosis of human carcinomas.

Human E-Cadherin/CDH1/E-cad/CD324 References
  • Wang HD, et al. (2004) CDH1 germline mutation in hereditary gastric carcinoma. World J Gastroenterol. 10(21): 3088-93.
  • Masterson J, et al. (2007) Posttranslational truncation of E-cadherin and significance for tumour progression. Cells Tissues Organs. 185(1-3): 175-9.
  • Mrgineanu E, et al. (2008) Correlation between E-cadherin abnormal expressions in different types of cancer and the process of metastasis. Rev Med Chir Soc Med Nat Iasi. 112(2): 432-6.
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    Catalog: 10204-RP02-50
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    All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"