Decorin / DCN / SLRR1B Antibody

Decorin / DCN / SLRR1B Antibody

Decorin / DCN / SLRR1B Antibody Product Information

• Immunogen: Recombinant human Decorin protein ( Catalog#10189-H08H )
• Antibody Type: Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
• Clone ID: 004
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant human Decorin ( rh Decorin ; Catalog#10189-H08H ; NP_001911.1 ; Met 1 - Lys 359 ).

Decorin / DCN / SLRR1B Antibody Usage Guide

• Specificity: Human Decorin / DCN / SLRR1B
• Western blot: This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect human Decorin in WB. Using a DAB detection system, the detection limit for human Decorin is approximately 1 ng/lane under non-reducing conditions and 0.5ng/lane under reducing conditions
• Direct ELISA: This antibody can be used at 0.1 - 0.2 μg/mL with the appropriate secondary reagents to detect human Decorin. The detection limit for human Decorin is approximately 0.0049 ng/well
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Decorin / DCN / SLRR1B Antibody Background

Decorin is a ubiquitous small cellular or pericellular matrix proteoglycan and is closely related in structure to biglycan protein. It belongs to the small leucine-rich proteoglycan (SLRP) family and consists of a core protein and a covalently linked glycosaminoglycan chain which is either chondroitin sulfate (CS) or dermatan sulfate (DS). As a component of connective tissue, decorin interacts with several extracellular matrix components, such as type I collagen and fibronectin, and plays a role in matrix assembly. The decorin core protein also binds to growth factors including TGF-beta, and to the decorin endocytosis receptor. Decorin is capable of suppressing the growth and the metastasis of a wide range of cancer cells in vitro through attenuation of the EGFR -mediated intacellular signaling and induction of apoptosis. Multiple alternatively spliced transcript variants for the decorin gene have been described. Mutations or deficiency of the decorin gene are associated with Marfan syndrome.

References

1. Pulkkinen, L. et al., 1990, J. Biol. Chem. 265: 17780-17785.
2. Dugan, T.A. et al., 2006, J. Biol. Chem. 281: 38208-38216.
3. Zafirpoulos, A. et al., 2008, Connect. Tissue. Res. 49: 244-248.
4. Li, X. e t al., 2008, Blood. 112: 159-168.
5. Krumdieck, R. et al., 1992, J. Immunol. 149: 3695-3701.