Rat DSG4 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
RG80273-G	Cloning vector ($295) pCMV / hygro ($545) pCMV / hygro - HA ($545) pCMV / hygro - His ($545) pCMV / hygro - Myc ($545) pCMV / hygro - FLAG ($545)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

DSG4 cDNA Clone Datasheet

DSG4 cDNA Clone Product Information

Gene Bank Ref.ID:	NM_199490.1
cDNA Size:	3123
Gene Synonym:	Dsg4
Source:	Rat

DSG4 cDNA Clone Description

cDNA Description:	ORF Clone of Rattus norvegicus desmoglein 4 DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref. ID sequence except for the point mutations: 2486 A/G resulting in the amino acid Glu substitution by Gly and 510 T/C, 693 T/C not causing the amino acid variation.

DSG4 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

DSG4 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

DSG4 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGACTGGC TTCTCTTCAG AAATATTTGC CTTCTGATCC TATTTATGGT GGTCCTAGGA
61       GTGAACAGTG AATTTATCGT TGAGGTAAAA GAATTAGACA TAGAAAACGG CACCACAACC
121      TGGCAGACTG TCAGGAGGCA GAAGCGAGAG TGGATCAAGT TTGCAGCGGC CTGTCGGGAG
181      GGCGAGGACA ACTCCAAGAG GAATCCGATT GCCAGAATTA GATCAGACTG TGAAGTGAGC
241      CAGAGGATCA CTTATCGAAT CTCTGGAGCG GGGATTGATC GGCCGCCTTA TGGAGTGTTC
301      ACCATCAACC CTCGGACTGG AGAAATCAAC ATTACCTCAG TAGTGGACAG AGAGATAACC
361      CCACTGTTCC TGATCCATTG CCGAGCTCTC AACTCGAGAG GAGAAGACCT GGAGAGACCG
421      CTGGAGCTCA GAGTGAAAGT CATGGATGTA AATGACAACC CTCCAGTCTT CACTCAGAAC
481      GTGTACACAG CCAACATTGA AGAAAACAGC GATGCAAATG CACTGGTGGT GAAGTTAAGT
541      GCCACGGATG CAGATGAAGA CAATCACTTG AATTCTAAAA TTGCTTACAA GATCATCTCC
601      CAGGAGCCTG CTGGTGCACC CATGTTCATG GTGAACAGGT ACACTGGAGA AGTCCGCACG
661      ATGTCCAACT TCCTTGACAG AGAGCAACAC AGCATGTACA ATCTCCTGGT GAGGGGCTCA
721      GATCGGGATG GAGCCACAGA TGGGCTGTCC TCTGAATGTG ACTGCAGAAT CAAGATTTTG
781      GATGTCAATG ATAATTTCCC CATCTTGGAG AAAACCTCAT ACTCAGCAAG TATTGAAGAA
841      AACTGTTTGA GCTCAGAACT GATACGACTG CAAGCAATCG ATCTCGATGA GGAAGGCACT
901      GATAACTGGC TGGCCCAATA CTCCATCCTC TCTGGCAATG ATGGGAACTG GTTTGAAATC
961      CAAACAGATC CAAAAACCAA CGAAGGCATT TTGAAATTGG TCAAGATGCT GGACTATGAA
1021     CAGGAACCTA ACATTTACCT CAGCATTGGA GTTAGAAACC AAGCTGAGTT TCACCATTCA
1081     GTGGCATCTC AATTCCAAAT GCACTCCACT CCCGTGAGAA TCCAGGTTAT TAACGTGCGG
1141     GAAGGACCAA CCTTTCGCCC AAGTTCCATG ACATTTAGTC TACGAGGAGG AATGAGGGGA
1201     GACTCCTTAA TGAACTATGT GCTTGGCACA TACACAGCCA TAGATATGGA CACGGGGAGC
1261     CCTGCAACAA ACGTCAGGTA TGTCATAGGG CACGATGCAG GCAGCTGGCT AAAAGTTGAT
1321     TCAAGGACTG GTGAGATACA GTTTTCTAGG GAATTTGACA CGAAGTCAAA ATATATTACG
1381     GATGGGATGT ATGCAGCAGA GATCCTGGCT ATAGATGATG GCTCTGGGAG AACAGCTACG
1441     GGAACCATAT GCATTGAGGT TCCTGATGCC AATGATTACT GTCCCGTCAT TTATGCCGAA
1501     AGTAGAAGTG TCTGCACCCA TGCTTCGTCT GTCAGGATCT ACGTCAACGA CCATTCTTTC
1561     GGGGCTCCCT TTACCTTTTG TGTGGTTGAC GAGTCGCCAG ACACAGCTGA CATATGGGAT
1621     ATCAGGTCCA TCAATGGAAC TTCTGCAATC CTTATGACTG AGCAGACCTT GTCTCCAGGA
1681     CCCTACCAAA TCCCCATCCT CGTGAAGGAC AGTCACAACA GAGCATGTGA ATTGCCACAG
1741     ACTGTACTGT TAGACGCCTG CTTGTGTGAC GACTACCATG TGTGCTTGCA CTCCAGCACC
1801     ACAGGCATCT ACACTGGGGA CACCATCTGG GTGACCGATG ACATGGGGAC GGTCACTGAT
1861     GATGGACTTA GGCAGTCAAA CGTCGGACTT GGCCCAGCGG GGATTGGCAT GATTATTTTG
1921     GGTCTCTTGC TGCTGTTCTT GTCACCCCTT TTACTGCTCA TGTGCTGCTG CAAGAGGAGA
1981     CAGCCAGAAG GCCTGGGGAC AAGGTTTGCC CCTGTGCCTG AGGGAGGCGA AGGAGTGATG
2041     CAGCCCTGGA GAATCGAGGG GGCTCATCCG GAAGACAGGG ATGTATCAAA TATATGTGTG
2101     CCCATGACAG CCTCTAACAC CCAAGATCGC ATTGATTCCT CTGAAATCTA CACCAACACT
2161     TATGCTGGTG GAGGAACAGT GGAAGGGGGT GTGTCAGGAG TGGAGCTCAA TACAGGTGTT
2221     GGGACAGCTG CAGGCATAGC AGCTGGAGGA GCAACAGGGA CCCTGAGAAA GAGGAGTTCA
2281     ACTATAGGGA CTCTTCGGGA ATACCAAGAC ACCGGCATGA ACATGGCCTT CCTGGACAGC
2341     TACTTCTCTG AGAAAGCGTA TGCGTATGCA GATGAAGATG AAGGCCGTCC AGCCAATGAT
2401     TGCCTGCTCA TTTATGACCA TGAAGGAACT GGCTCACCCG TGGGCTCCAT TGGCTGCTGC
2461     AGTTGGATTG TGGATGACTT AGATGGGAGC TACATGGAGA CTTTAGATCC AAAGTTCAGG
2521     ACTCTAGCTG AAATCTGCTT GGACACAGAG ATTGAACCAT TCCCCTCACA CCAGGCCTGT
2581     ATACCAATAA GCACTGACCT CCCTCTGCTG GGGCCTAATT ACTTTGTCAA TGAATCTTCA
2641     GGAATGACTC TCTCGGAGGC TGAATTCCAA GCAGAAATGG CAGCAGCCTC TGAACCCATG
2701     ATTCATGGGG ACATCATAGT GACTGAAACT TACACTGCCA CTGACCCATG TGTACAACCC
2761     ACGACGATTG TCTTTGACTC TCAGCTTCCA CCCAATGTAG TCGTGACCGA AACCGTGATG
2821     GCACCTGTCT ATGATGTTCA AGGGAACATC TGTGTGCCTG CCGAGATAGC CAACACACAC
2881     AATGTATACT ACGCTGAGAG AGTGGTGGCT AGCCCTGGTG TGCCTGACAT GGGCAACGGT
2941     AACATAGGTG ATACATGTAT AGGACCAGTG ATGAGTGGTG GTATTTTGGT AGGGCCAGAA
3001     ATTCAAGTGA CACAAATGAT GAGCCCAGAC ATTCACATAA GCCAAACCAC ACGCTCTACC
3061     TCCCCAATGA CGTCTCAACA CCGAGTAACC AGATACAGCA ACATACACTA CTCCCGACAG
3121     TAA

Quality Control:cDNA sequencing results is available upon request.