Human DSG3 Gene cDNA Clone / ORF Clone

DSG3 cDNA Clone Datasheet

DSG3 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_001944.2
• cDNA Size: 3000
• Gene Synonym: PVA, CDHF6, DKFZp686P23184
• Source: Human

DSG3 cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens desmoglein 3 (pemphigus vulgaris antigen) DNA.
• Vector: pGEM-T Vector

• Sequence Description: Same as Gene Bank Ref. ID sequence with the nucleotide 1858 G deletion mutation, the point mutation 2734 A/G resulting in the amino acid thr substitution by ala and the point mutation 960 T/A not causing the amino acid variation.

DSG3 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

DSG3 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

DSG3 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGATGGGGC TCTTCCCCAG AACTACAGGG GCTCTGGCCA TCTTCGTGGT GGTCATATTG
61       GTTCATGGAG AATTGCGAAT AGAGACTAAA GGTCAATATG ATGAAGAAGA GATGACTATG
121      CAACAAGCTA AAAGAAGGCA AAAACGTGAA TGGGTGAAAT TTGCCAAACC CTGCAGAGAA
181      GGAGAAGATA ACTCAAAAAG AAACCCAATT GCCAAGATTA CTTCAGATTA CCAAGCAACC
241      CAGAAAATCA CCTACCGAAT CTCTGGAGTG GGAATCGATC AGCCGCCTTT TGGAATCTTT
301      GTTGTTGACA AAAACACTGG AGATATTAAC ATAACAGCTA TAGTCGACCG GGAGGAAACT
361      CCAAGCTTCC TGATCACATG TCGGGCTCTA AATGCCCAAG GACTAGATGT AGAGAAACCA
421      CTTATACTAA CGGTTAAAAT TTTGGATATT AATGATAATC CTCCAGTATT TTCACAACAA
481      ATTTTCATGG GTGAAATTGA AGAAAATAGT GCCTCAAACT CACTGGTGAT GATACTAAAT
541      GCCACAGATG CAGATGAACC AAACCACTTG AATTCTAAAA TTGCCTTCAA AATTGTCTCT
601      CAGGAACCAG CAGGCACACC CATGTTCCTC CTAAGCAGAA ACACTGGGGA AGTCCGTACT
661      TTGACCAATT CTCTTGACCG AGAGCAAGCT AGCAGCTATC GTCTGGTTGT GAGTGGTGCA
721      GACAAAGATG GAGAAGGACT ATCAACTCAA TGTGAATGTA ATATTAAAGT GAAAGATGTC
781      AACGATAACT TCCCAATGTT TAGAGACTCT CAGTATTCAG CACGTATTGA AGAAAATATT
841      TTAAGTTCTG AATTACTTCG ATTTCAAGTA ACAGATTTGG ATGAAGAGTA CACAGATAAT
901      TGGCTTGCAG TATATTTCTT TACCTCTGGG AATGAAGGAA ATTGGTTTGA AATACAAACA
961      GATCCTAGAA CTAATGAAGG CATCCTGAAA GTGGTGAAGG CTCTAGATTA TGAACAACTA
1021     CAAAGCGTGA AACTTAGTAT TGCTGTCAAA AACAAAGCTG AATTTCACCA ATCAGTTATC
1081     TCTCGATACC GAGTTCAGTC AACCCCAGTC ACAATTCAGG TAATAAATGT AAGAGAAGGA
1141     ATTGCATTCC GTCCTGCTTC CAAGACATTT ACTGTGCAAA AAGGCATAAG TAGCAAAAAA
1201     TTGGTGGATT ATATCCTGGG AACATATCAA GCCATCGATG AGGACACTAA CAAAGCTGCC
1261     TCAAATGTCA AATATGTCAT GGGACGTAAC GATGGTGGAT ACCTAATGAT TGATTCAAAA
1321     ACTGCTGAAA TCAAATTTGT CAAAAATATG AACCGAGATT CTACTTTCAT AGTTAACAAA
1381     ACAATCACAG CTGAGGTTCT GGCCATAGAT GAATACACGG GTAAAACTTC TACAGGCACG
1441     GTATATGTTA GAGTACCCGA TTTCAATGAC AATTGTCCAA CAGCTGTCCT CGAAAAAGAT
1501     GCAGTTTGCA GTTCTTCACC TTCCGTGGTT GTCTCCGCTA GAACACTGAA TAATAGATAC
1561     ACTGGCCCCT ATACATTTGC ACTGGAAGAT CAACCTGTAA AGTTGCCTGC CGTATGGAGT
1621     ATCACAACCC TCAATGCTAC CTCGGCCCTC CTCAGAGCCC AGGAACAGAT ACCTCCTGGA
1681     GTATACCACA TCTCCCTGGT ACTTACAGAC AGTCAGAACA ATCGGTGTGA GATGCCACGC
1741     AGCTTGACAC TGGAAGTCTG TCAGTGTGAC AACAGGGGCA TCTGTGGAAC TTCTTACCCA
1801     ACCACAAGCC CTGGGACCAG GTATGGCAGG CCGCACTCAG GGAGGCTGGG GCCTGCCGCC
1861     ATCGGCCTGC TGCTCCTTGG TCTCCTGCTG CTGCTGTTGG CCCCCCTTCT GCTGTTGACC
1921     TGTGACTGTG GGGCAGGTTC TACTGGGGGA GTGACAGGTG GTTTTATCCC AGTTCCTGAT
1981     GGCTCAGAAG GAACAATTCA TCAGTGGGGA ATTGAAGGAG CCCATCCTGA AGACAAGGAA
2041     ATCACAAATA TTTGTGTGCC TCCTGTAACA GCCAATGGAG CCGATTTCAT GGAAAGTTCT
2101     GAAGTTTGTA CAAATACGTA TGCCAGAGGC ACAGCGGTGG AAGGCACTTC AGGAATGGAA
2161     ATGACCACTA AGCTTGGAGC AGCCACTGAA TCTGGAGGTG CTGCAGGCTT TGCAACAGGG
2221     ACAGTGTCAG GAGCTGCTTC AGGATTCGGA GCAGCCACTG GAGTTGGCAT CTGTTCCTCA
2281     GGGCAGTCTG GAACCATGAG AACAAGGCAT TCCACTGGAG GAACCAATAA GGACTACGCT
2341     GATGGGGCGA TAAGCATGAA TTTTCTGGAC TCCTACTTTT CTCAGAAAGC ATTTGCCTGT
2401     GCGGAGGAAG ACGATGGCCA GGAAGCAAAT GACTGCTTGT TGATCTATGA TAATGAAGGC
2461     GCAGATGCCA CTGGTTCTCC TGTGGGCTCC GTGGGTTGTT GCAGTTTTAT TGCTGATGA

 

Quality Control:cDNA sequencing results is available upon request.