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The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.
The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.
|Rat DSC2 Gene cDNA Clone (full-length ORF Clone), expression ready, FLAG-tagged||RG80266-G-F|
|Rat DSC2 Gene cDNA Clone (full-length ORF Clone), expression ready, His-tagged||RG80266-G-H|
|Rat DSC2 Gene cDNA Clone (full-length ORF Clone), expression ready, Myc-tagged||RG80266-G-M|
|Rat DSC2 Gene cDNA Clone (full-length ORF Clone), expression ready, untagged||RG80266-G-N|
|Rat DSC2 Gene cDNA Clone (full-length ORF Clone), expression ready, HA-tagged||RG80266-G-Y|
DSC2 is a calcium-dependent glycoprotein that is a member of the desmocollin subfamily of the cadherin superfamily. Like other desmocollins, murine DSC2 has two products, Dsc2a and Dsc2b, produced by alternative splicing of a 46 bp exon which encodes 11 COOH-terminal aa followed by an in-frame stop codon. These desmosomal family members, along with the desmogleins, are found primarily in epithelial cells where they constitute the adhesive proteins of the desmosome cell-cell junction and are required for cell adhesion and desmosome formation. The desmosomal family members are arranged in two clusters on chromosome 18, occupying less than 650 kb combined. Mutations in DSC2 are associated with arrhythmogenic right ventricular dysplasia-11. DSC2 is Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. DSC2 may contribute to epidermal cell positioning by mediating differential adhesiveness between cells that express different isoforms.