Rat DSC2 Gene cDNA Clone / ORF Clone

DSC2 cDNA Clone Datasheet

DSC2 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_001033688.1
• cDNA Size: 2709
• Gene Synonym: Dsc2
• Source: Rat

DSC2 cDNA Clone Description

• cDNA Description: ORF Clone of Rattus norvegicus desmocollin 2 DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

DSC2 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

DSC2 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

DSC2 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCGGCAG CGGGATCCTC GCGCTCCGGG AGCCCTGCCC TCGGCCTGGG ACACCTGTTG
61       ACCCTCGCGA TCCTTGCACT TGCCTGTGAT GCTTGTAAAG AAGTCGTCCT CCAGGTCCCC
121      TCTGAACTAC CTGCTGGGAA ATTTGTTGGC AGAGTGAACC TGATGGAGTG CCTTAAATCA
181      GCAAATGTAA TTCACTTGAG TGATCCTGAT TTCCAAGTCT TCGAAGATGG TTCTGTGTAC
241      ACAACCAATT CTGTTGTTTT GTCTTCGGGG CAAAGAAGCT TTACTATTTG GCTTTTCAAC
301      ACAGACAGCC AGGAAGAGAG GGAGTTGTCT GTCCATTTAG AGGGCCCTGT AGAGGTCCTA
361      AATCAAAGAC TGCATACAGA AAAAGTTCTC CGCCGCGCCA AGAGAAGATG GGCTCCCATC
421      CCTTGTTCCA TGATGGAGAA TTCATTGGGT CCCTTCCCAC TTTTCCTTCA ACAGATCCAA
481      TCCGACAAAG CTCAAAACTA CACCATATAC TATTCCATCA GCGGTCCAGG AGTTGATAAA
541      GAACCCGTAG GCTTATTTCG TATTGATAGA GATACTGGAA ACCTGTATTG CACTGATCGT
601      GTAGATCGAG AACAGTATGA CTCCTTTGAG CTGACTGCAT TAGCAATGAC TCCAGATGGG
661      TATATGGTAG AGAACCTGCT GACGCTCCTC ATTAAAGTAG AGGATGAGAA CGATAATTAC
721      CCGATCTTTA CCCAGAAAGT GTACAGCTTC ACAGTTTTGG AAAACAGTCC ATTTGGTTCT
781      CACGTCGGGG AGGTCTGTGC AACAGACAAG GATGAGCCGG ACACAATGCA CACTCGTCTG
841      AAGTATTCCA TCCTGGAGCA GTCGCCTTCT CCCCCCATGT TATTCACCAT GCATCCCAGG
901      ACTGGTGTCA TCACTACCAC CTCCACCCAG CTGGACAGAG AGTTAATTGA CAAGTACCAG
961      TTGATAATAA AAGTTCAGGA CATGGATGGC CAGTATTTTG GTTTGCAGAC AACAGCGACC
1021     TGCGTCATTA CTATTGGAGA TGTGAATGAC AACTTGCCAA CATTCACGCG TACTACGTAC
1081     GTGACATCAG TGGAAGAAAA TACGGTCAAT GTGGAACTCT TACGTCTTTC TGTTCAAGAT
1141     AAAGATTTAG TTAACTCTCC TAATTGGAGA GCTAATTACA CCATTTTAAA GGGCAACGAA
1201     AATGGAAATT TTAAAATCAA AACAGATCCC CAAACCAATG AAGGCATTCT GTGCGTGATT
1261     ATGCCTCTGG ACTATGAAGA ACGGCAACGG GTGACCCTGG AAATTGGCGT AGTTAATGAA
1321     GCCCCGTACA CTAGACAGGC TGGTTCAAAG TCATCCATGA GCACAGCTAC AGTGACTGTC
1381     AACGTGAAAA ATCAGGATGA GGGCCCTGAG TGTATCCCTC AAACGCAAAC TGTGAGGATT
1441     CGAGAAAATG TACCAATTGG TACTAGAAAC GATGGGTATA AAGCATATGA CCCAGAGACC
1501     AAAAACAGCA TTGGCATAAG GTACAGGAAG TTAAGTGACC CAAAAGGATG GATCACTGTT
1561     AATGAAGATT CAGGATCAAT CACCATTTTC CGAACTCTGG ATCGGGAGGC TGAGACCATC
1621     AGAAACGGCA TCTACAATAT TACAGTCCTT GCATTAGACG CAGACGGGAG AAGCTGTACG
1681     GGAACTCTGG GAATCTTACT TGAAGATGTG AATGACAACG GCCCATTCAT ACCCAAGCAG
1741     ACAGTGGTGA TATGCAGGGC TACCATGTCC TCTGCTGAAA TTGTTGCGGT TGACCCTGAT
1801     GAGCCAGCAA ATGGTCCACC ATTTGATTTT AGTTTGGAGA GTTCTGATTC AGAAGTACGG
1861     AGGAGGTGGA AGTTGACAAG AATCAATGAT ACTGCTGCTA GTCTTTCCTA TCAGAATGAC
1921     CCTGCGTTTG ATTCCTACGT AATTCCCGTC CGAGTTACAG ACAGGTTTGG TCTGTCCTCA
1981     GTCACGTCAC TGAATGTCGT TGTATGTGAC TGCATCACTG AAAGTGACTG CACATACCGC
2041     ACTGGAGAAA GGACTGGCTA TGCTGACGTG AGACTTGGAC CATGGGCTAT CCTTGCTATA
2101     CTGCTGGGCG TAGCCTTGCT ACTTTGTATC CTGTTTACCT TAGTCTGTAG TGTTTCCCGG
2161     GCATCAAAAC AACAGAAAAT TCTTCCCGAT GACTTAGCTC AGCAGAACCT AATTGTATCA
2221     AACACCGAGG CTCCTGGAGA TGACAAAGTC TATTCCGCAA ATGGCTTCAC AGCTCAAACT
2281     GCGGGAGCTT CTGGGCAGAC AACATTCACA ACCGTGGGAG CTGGAGTCAA AAGTACAGGG
2341     CAAGAGACCA TTGAGATGGT AAAAGGAGGA CAGCGCACAC TGGACTCCCG CAGGGCGACC
2401     GGCTATCACC ACCATACCAT GGATCCTGGC AGGGGAGGGC ATGTGGAGGT GGACAATTAC
2461     AGATACACCT ACTCTGAGTG GCACAATTTC ACTCAGCCCC GACTTGGTGA GAAGGTGCAG
2521     TTTTGCCATG AGGATGACAA CCAGAAGCTT GCCCAAGACT ATGTCCTCAC ATACAACTAT
2581     GAGGGAAAAG GCTCGGCTGC TGGCTCTGTG GGTTGCTGTA GCGACCTACA GGAAGAAGAC
2641     GGGCTTGAGT TTTTGGACCA TCTGGAGCCT AAATTTAGGA CATTAGCAGA AGTATGTGCA
2701     AAGAGGTGA

 

Quality Control:cDNA sequencing results is available upon request.