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> ELISA > ELISA Pair Set > DPP7 / DPPII / DPP2 ELISA Pair Set, Human ELISA Pair Set DPP7 / DPPII / DPP2 ELISA Pair Set, Human ELISA Pair Set 
| Catalog | Size (Price) | Quantity | In Stock | Operation | Other Information |
| SEK10748 |
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Human DPP7 / DPPII / DPP2 ELISA Pair Set
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Order or Inquire for DPP7 / DPPII / DPP2 ELISA Pair Set |
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High affinity Human DPP7 / DPPII / DPP2 ELISA Pair Set |
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Detection limit - 62.5 pg/ml |
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Affordable price and 30%-80% cost saving for Bulk order |
- Materials
- Protocol
- Trouble Shooting
- Image & Flash
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No signal |
Incorrect or no Detection Antibody was added |
Add appropriate Detection Antibody and continue |
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Substrate solution was not added |
Add substrate solution and continue |
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Incorrect storage condition |
Check if the kit is stored at recommended condition and used before expiration date |
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Poor Standard Curve |
Standard was incompletely reconstituted or was inappropriately stored |
Aliquot reconstituted standard and store at -70 ℃ |
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Imprecise / inaccurate pipetting |
Check / calibrate pipettes |
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Incubations done at inappropriate temperature, timing or agitation |
Follow the general ELISA protocol |
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Background wells were contaminated |
Avoid cross contamination by using the sealer appropriately |
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Poor detection value |
The concentration of antigen in samples was too low |
Enriching samples to increase the concentration of antigen |
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Samples were ineffective |
Check if the samples are stored at cold environment. Detect samples in timely manner |
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High Background |
Insufficient washes |
Use multichannel pipettes without touching the reagents on the plate |
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Increase cycles of washes and soaking time between washes |
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TMB Substrate Solution was contaminated |
TMB Substrate Solution should be clear and colorless prior to addition to wells |
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Materials were contaminated |
Use clean plates, tubes and pipettes tips |
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Non-specificity |
Samples were contaminated |
Avoid cross contamination of samples |
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The concentration of samples was too high |
Try higher dilution rate of samples |
Problems 
Possible Sources 
Solutions 
Human DPP7 / DPPII / DPP2 ELISA Pair Set Related Products & Topics
Related Areas:
Enzyme>>Protease & Regulator>>Serine Protease & Regulator>>DPPIV Family>>DPP7/DPPII/DPP2
Proteins:
| Molecule | Species | Description //For Detailed Info. and Price------CLICK! | Cat. No |
| DPP7/DPPII/DPP2 | Human | DPP7/DPPII/DPP2 Protein, Recombinant | 10748-H08H |
Antibodies:
| Molecule | Application | Description //For Detailed Info. and Price------CLICK! | Cat. No |
| Human DPP7/DPPII/DPP2 | WB | Mouse Monoclonal Antibody | 10748-MM02 |
| Human DPP7/DPPII/DPP2 | ELISA | Mouse Monoclonal Antibody | 10748-MM03 |
| Human DPP7/DPPII/DPP2 | ELISA | Rabbit Polyclonal Antibody | 10748-RP01 |
| Human DPP7/DPPII/DPP2 | WB, ELISA | Rabbit Polyclonal Antibody (Antigen Affinity Purified) | 10748-RP04 |
Background
Dipeptidyl-peptidase II (DPPII), also known as quiescent cell proline dipeptidase (QPP) and dipeptidyl-peptidase 7 (DPP7), is a post-proline cleaving amino peptidase expressed in quiescent lymphocytes. As an intracellular protease localized to the vesicular system, it releases preferably N-terminal dipeptides from oligopeptides with Pro or Ala in the penultimate position at acidic pH.. The proline-specific DPPs like DPP7, fibroblast activation protein alpha (FAP), DPPIV, DPP8 and DPP9, on the basis of their preference for cleavage after X-Pro, are likely to be involved in Pro-specific N-terminal processing regulating an increasing number of biological processes. These DPPs are emerging as an important protease family involved in the regulation of signaling by peptide hormones. The purified rhDPP7 is active against Lys-Pro-AMC and Lys-Ala-AMC. Its activity against Lys-Pro-AMC is approximately 10-fold of that against Lys-Ala-AMC under identical conditions.
