Cynomolgus monkey DPP4 Gene cDNA Clone / ORF Clone

DPP4 cDNA Clone Datasheet

DPP4 cDNA Clone Product Information

• cDNA Size: 2301
• Gene Synonym: DPP4
• Source: Cynomolgus monkey

DPP4 cDNA Clone Description

• cDNA Description: ORF Clone of Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) dipeptidyl-peptidase 4 DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutations: 131 T/C, 544 G/A, 2252 C/T resulting in the amino acid Ile substitution by Thr, Gly substitution by Ser, Thr substitution by Ile and 1086 C/T, 2052 A/G not causing the amino acid variation.
  Please check the sequence information before order.

DPP4 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

DPP4 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

DPP4 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAAGACAG CGTGGAAGGT TCTCCTGGGA CTGCTGGGTG CTGCTGCGCT TGTCACCATC
61       ATCACCGTGC CCGTGGTTCT GCTGAACAAA GGCACAGATG ATGCTACAGC TGACAGTCGC
121      AAAACTTACA TTCTAACTGA TTACTTAAAA AATACTTATA GACTGAAGTT GTACTCCTTA
181      CGATGGATTT CAGATCATGA ATATCTCTAC AAACAAGAAA ATAATATCTT GGTATTCAAT
241      GCTGAATATG GAAACAGCTC GGTTTTCTTG GAGAACAGTA CATTTGATGA GTTTGGACAT
301      TCTATAAATG ATTATTCAAT ATCTCCTGAT GGGCAGTTTA TTCTCTTAGA ATACAACTAT
361      GTGAAGCAAT GGAGGCATTC CTACACAGCT TCATATGACA TTTATGATTT AAATAAAAGG
421      CAGCTGATTA CAGAAGAGAG GATTCCAAAC AACACACAGT GGGTCACATG GTCACCAGTG
481      GGTCATAAAT TGGCATATGT TTGGAACAAT GACATTTATG TTAAAATTGA ACCAAATTTA
541      CCAAGTCACA GAATCACATC GACTGGGAAA GAAGATATGA TATATAATGG AATAACTGAC
601      TGGGTTTATG AAGAGGAAGT CTTCAGTGCC TACTCTGCTC TGTGGTGGTC TCCAAACGGC
661      ACTTTTTTAG CATATGCCCA ATTTAACGAC ACAGAAGTCC CACTTATTGA ATACTCCTTC
721      TACTCTGATG AGTCACTGCA GTACCCAAAG ACTGTACAGG TTCCATATCC AAAGGCAGGA
781      GCTGTGAATC CAACTGTAAA GTTCTTTGTT GTAAATACAG ACTCTCTCAG CTCAGCCACC
841      AATGCAACTT CCATACAAAT CACTGCGCCT GCTTCTATGT TGATAGGGGA TCACTACTTG
901      TGTGATGTGA CATGGGCAAC ACAAGAAAGA ATTTCTTTGC AGTGGCTCAG GAGGATTCAG
961      AACTATTCGG TCATGGATAT TTGTGATTAT GATGAGTCCA GTGGAAGATG GAACTGCTTA
1021     GTGGCACGGC AACACATTGA AACGAGTACT ACTGGCTGGG TTGGAAGATT TAGGCCTTCG
1081     GAACCTCATT TTACCTCTGA TGGTAATAGC TTCTACAAGA TCATCAGCAA TGAAGAAGGT
1141     TACAGACACA TTTGTTATTT TCAAATAAAT AAAAAAAACT GCACATTTAT TACAAAAGGA
1201     GCCTGGGAAG TCATCGGGAT AGAAGCTCTA ACCAGCGATT ATCTATACTA CATTAGTAAT
1261     GAATATAAAG GAATGCCAGG AGGAAGGAAT CTTTATAAAA TCCAACTTAG TGACTATACA
1321     AAAGTGACAT GCCTTAGTTG TGAGCTGAAT CCGGAAAGGT GTCAGTACTA TTCTGTGTCA
1381     TTCAGTAAAG AGGCGAAGTA TTATCAGCTG AGATGTTCCG GTCCTGGTCT GCCCCTCTAT
1441     ACTCTACACA GCAGTGTGAA TGATAAAGGG CCGAGAGTCC TGGAAGATAA TTCAGCTTTG
1501     GATAAAATGC TGCAGAATGT CCAGATGCCC TCCAAAACAC TGGACTTCAT TATTTTGAAT
1561     GAAACAAAAT TTTGGTATCA GATGATCTTG CCTCCCCATT TTGATAAATC CAAGAAATAT
1621     CCTCTACTAT TAGATGTGTA TGCAGGCCCG TGTAGTCAAA AAGCAGACGC TGTCTTCAGA
1681     CTCAACTGGG CCACTTACCT TGCAAGCACA GAAAACATTA TAGTAGCTAG CTTTGATGGC
1741     AGAGGAAGTG GTTACCAAGG AGATAAGATC ATGCATGCAA TCAACAGAAG ACTGGGAACA
1801     TTTGAAGTTG AAGATCAAAT TGAAGCAGCC AGACAATTTT CAAAAATGGG ATTTGTGGAC
1861     AACAAACGAA TTGCAATTTG GGGCTGGTCA TATGGAGGGT ACGTAACCTC AATGGTCCTG
1921     GGATCGGGAA GCGGCGTGTT CAAGTGTGGA ATAGCCGTGG CGCCTGTATC CCGGTGGGAG
1981     TACTATGATT CAGTGTACAC AGAACGTTAC ATGGGTCTCC CAACTCCAGA AGACAACCTT
2041     GACCATTACA GGAATTCAAC AGTCATGAGC AGAGCTGAAA ATTTTAAACA AGTTGAGTAC
2101     CTCCTTATTC ATGGAACAGC AGATGATAAC GTTCACTTTC AGCAGTCAGC TCAGATCTCC
2161     AAAGCCCTGG TCGATGCTGG AGTGGATTTC CAGGCAATGT GGTATACTGA TGAAGACCAT
2221     GGAATCGCTA GCAGCACAGC ACACCAACAC ATATACACCC ACATGAGCCA CTTCATAAAA
2281     CAATGTTTCT CTTTACCTTA G

 

Quality Control:cDNA sequencing results is available upon request.