DOT1L (KMT4)

DOT1L is histone methyltransferase. DOT1L methylates 'Lys-79' of histone H3. DOT1L nucleosomes are preferred as substrate compared to free histones. DOT1L binds to DNA.

DOT1L (KMT4) Related Areas

Enzyme>>Methyltransferase>>DOT1L/KMT4

DOT1L (KMT4) Alternative Names

DOT1L, KMT4, DKFZp586P1823, DOT1, KIAA1814 [Homo sapiens]

Dot1l, KMT4, A630076O07, AW907654, Dot1, mDot1 [Mus musculus]

Summaries for DOT1L (KMT4)

Entrez Gene summary for DOT1L:

The protein encoded by this DOT1L gene is a histone methyltransferase that methylates lysine-79 of histone H3. DOT1L is inactive against free core histones, but shows significant histone methyltransferase activity against nucleosomes. [provided by RefSeq, Aug 2011]

Wikipedia summary for DOT1L:

DOT1-like, histone H3 methyltransferase (S. cerevisiae), also known as DOT1L, is a gene found in humans, as well as other eukaryotes.

Human DOT1L (KMT4) Protein General Information

 

• Protein names: Histone-lysine N-methyltransferase, H3 lysine-79 specific
• Sequence length: 1739 AA.
• Catalytic activity: S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone].
• Sequence similarities: DOT1L belongs to the class I-like SAM-binding methyltransferase superfamily. DOT1 family. DOT1L contains 1 DOT1 domain.
• Post-translational modification: In contrast to other lysine histone methyltransferases, it does not contain a SET domain, suggesting the existence of another mechanism for methylation of lysine residues of histones.
• Subunit structure: DOT1L interacts with MLLT10.
• Subcellular location: Nucleus
• Tissue specificity: Miscellaneous
• Sequence caution: The sequence AAC08316.1 differs from that shown. Reason: Erroneous gene model prediction.

General information above from UniProt

Function for DOT1L (KMT4) Protein

UniProtKB:

DOT1L is histone methyltransferase. DOT1L methylates 'Lys-79' of histone H3. DOT1L nucleosomes are preferred as substrate compared to free histones. DOT1L binds to DNA.

Genatlas:

• DOT1L plays a role in nucleosomal histone methylation
• DOT1L methylates Lys-79 of histone H3
• recruitment important for transformation by the MLL-MLLT1 fusion derivative (Pubmed 17855633)
• DOT1L can promote an oncogenic pattern of gene expression through binding with several MLL fusion partners found in acute leukemia (Pubmed 18285465)
• through H3K79 methylation, DOT1L plays an important roles in heterochromatin formation and in embryonic development (Pubmed 18787701)
• DOT1L is a unique histone methyltransferase that specifically methylates histone H3 at lysine 79 (Pubmed 20798234)
• DOT1L-dependent H3K79 methylation serves as a critical regulator of a differentiation switch during early hematopoiesis (Pubmed 20798234)
• DOT1L is involved in the regulation of telomeric silencing, development, cell cycle checkpoint and transcription (Pubmed 20431927)
• DOT1L might control AF9- and ENL-mediated transcription, regulate RNA processing, and function as a histone chaperone in a NPM1-dependent manner (Pubmed 20431927)
• DOT1L is responsible for methylation of histone H3 at lysine 79 and is involved in the pathobiology of several leukemias, the majority of which are characterized by chromosomal translocations involving the mixed lineage leukemia (MLL) gene (Pubmed 20230194)
• DOT1L may contribute to euchromatin formation or transcription (Pubmed 20678485)
• DOT1L-dependent lysine 79 of histone H3 methylation serves potentially as a critical regulator of a differentiation switch during early hematopoiesis (Pubmed 20798234)
• DOT1L plays a critical role for DOT1L-mediated H3K79 methylation in cardiomyocyte function (Pubmed 21289070)
• H3K79 methyltransferase, DOT1L is required for both initiation and maintenance of MLL-AF9-induced leukemogenesis (Pubmed 2152178)

Homology for human DOT1L (KMT4)

• homolog to yeast Dot1
• ortholog to murine Dot1l