DDC Matched ELISA Antibody Pair Set,Human
Solid Phase Sandwich ELISA
Quantitative determination of Human DDC
1. Capture Antibody 0.5 mg/mL of rabbit anti-Human DOPA Decarboxylase / DDC monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating. (Catalog: # 10560-R003)
2. Detection Antibody 0.2 mg/mL of rabbit anti-Human DOPA Decarboxylase / DDC monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.3 μg/mL in detection antibody dilution buffer before use. (Catalog: # 10560-R040)
3. Standard Each vial contains 165 ng of recombinant Human DOPA Decarboxylase / DDC. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 7000 pg/mL is recommended.
This DDC Matched ELISA Antibody Pair Set,Human is a solid phase sandwich ELISA for quantitative determination of Human DDC . It contains Human DDC capture antibody, Human DDC detector antibody
and a highly purified
recombinant Human DDC protein. This Pair Set is at affordable price for researchers.
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
Dopa Decarboxylase (DDC), also known as AADC and Aromatic-L-amino acid decarboxylase, is a 54 kDa member of the group II decarboxylase family of proteins.It is a vitamin B6-dependent homodimeric enzyme that catalyzes the decarboxylation of both L-3,4-dihydroxyphenylalanine (L-DOPA) and L-5-hydroxytryptophan to dopamine and serotonin, respectively, which are major mammalian neurotransmitters and hormones belonging to catecholamines and indoleamines. Since L-DOPA is regularly used to treat the symptoms of Parkinson's disease, the catalytic pathway is of particular research interest. Defects of DDC are associated with severe developmental delay, oculogyric crises (OGC), as well as autosomal recessive disorder AADC deficiency, an early onset inborn error in neurotransmitter metabolism which can lead to catecholamine and serotonin deficiency.
Ichinose, H. et al.,1989,Biochem. Biophys. Res. Commun. 164: 1024-1030. Lisa, J. S. et al., 1992, Genomics 13: 469-471. Moore, P. S. et al.,1996, Biochem. J. 315:249-256. Bertoldi, M. et al., 2003, Biochim. Biophys. Acta. 1647:42-47. Vassilacopoulou, D. et al., 2004, Neurochem. Res. 29: 1817-1823. Ma, J.Z., et al., 2005, Hum. Mol. Genet. 14: 1691-1698.