|Recombinant Human DDC protein (Catalog#10560-H08B)|
|0.2 μm filtered solution in PBS with 5% trehalose|
|This antibody was obtained from a rabbit immunized with purified, recombinant Human DDC / AADC (rh DDC; Catalog#10560-H08B; NP_000781.1; Met 1-Glu 480).|
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human DDC. The detection limit for Human DDC is approximately 0.00245 ng/well.
ELISA(Det): 0.1-1 µg/ml
This antibody will detect Human DOPA Decarboxylase / DDC in ELISA pair set (Catalog: # SEK10560). In a sandwich ELISA, it can be used as detection antibody when paired with (Catalog: # 10560-R003).
Dopa Decarboxylase (DDC), also known as AADC and Aromatic-L-amino acid decarboxylase, is a 54 kDa member of the group II decarboxylase family of proteins.It is a vitamin B6-dependent homodimeric enzyme that catalyzes the decarboxylation of both L-3,4-dihydroxyphenylalanine (L-DOPA) and L-5-hydroxytryptophan to dopamine and serotonin, respectively, which are major mammalian neurotransmitters and hormones belonging to catecholamines and indoleamines. Since L-DOPA is regularly used to treat the symptoms of Parkinson's disease, the catalytic pathway is of particular research interest. Defects of DDC are associated with severe developmental delay, oculogyric crises (OGC), as well as autosomal recessive disorder AADC deficiency, an early onset inborn error in neurotransmitter metabolism which can lead to catecholamine and serotonin deficiency.