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人 DNMT1 基因全长ORF克隆

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Human DNMT1 cDNA Clone Product Information
NCBI RefSeq:NM_001130823.1
RefSeq ORF Size:4899bp
cDNA Description:Full length Clone DNA of Homo sapiens DNA.(cytosine-5-)-methyltransferase 1.
Gene Synonym:AIM, DNMT, MCMT, CXXC9, FLJ16293, MGC104992, DNMT1
Species:Human
Vector:pMD18-T Vector
Plasmid:pMD-DNMT1
Restriction Site:
Tag Sequence:
Sequence Description:Identical with the Gene Bank Ref. ID sequence except for five point mutations: 6 G/A, 1389 A/G ,1782 A/G not causing the amino acid variation and 979 A/G resulting in the 327 amino acid Ile substitution by Val and 3356 C/T resulting in the amino 1119 acid Ala substitution by Val.
Sequencing primers:M13-47 and RV-M
Promoter:
Application:
Antibiotic in E.coli:Ampicilin
Antibiotic in mammalian cell:
Shipping_carrier:Each tube contains lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at room temperature for three months.
pMD18-T Vector Information

pMD18-T Vector is a high-efficiency TA cloning vector constructed from pUC18, of which multiple cloning sites as shown below. The pMD18-T Vector is 2.6kb in size and contains the amplicin resistance gene for selection. The coding sequence was inserted by TA cloning at site 425.

pMD18-T vector Usage Suggestion:

The coding sequence can be amplified by PCR with M13-47 and RV-M primers.

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Catalog: HG11494-M
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