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Human DNMT1 Gene cDNA Clone / ORF Clone
| Catalog | Vector (Price) | Quantity | In Stock | Operation |
| HG11494-M |
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YES |  |
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* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. |
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DNMT1 cDNA Clone Datasheet
DNMT1 cDNA Clone Product Information
| Gene Bank Ref.ID: | NM_001130823.1 |
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cDNA Size: |
4899 |
| Gene Synonym: |
AIM; DNMT; MCMT; CXXC9; FLJ16293; MGC104992; DNMT1 |
| Source: | Human |
DNMT1 cDNA Clone Description
|
cDNA Description: |
ORF Clone of Homo sapiens DNA (cytosine-5-)-methyltransferase 1 DNA. |
| Vector: | Cloning Vector: pMD18-T Vector.
Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG |
| Sequence Description: | Identical with the Gene Bank Ref. ID sequence except for five point mutations: 6 G/A, 1389 A/G ,1782 A/G not causing the amino acid variation and 979 A/G resulting in the 327 amino acid Ile substitution by Val and 3356 C/T resulting in the amino 1119 acid Ala substitution by Val. |
DNMT1 cDNA Clone Shipping
- 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
- It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.
DNMT1 cDNA Clone Usage Protocol
- Cut along the circle edge of the spot (arrow points to) to get the filter paper.
- Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O . Ensure the filter paper is completely immersed in the H2O by briefly centrifuging the tube for 10 seconds.
- Transfer the tube to incubation at 95℃ for 15-30 min.
- At the end of the incubation pulse vortex, or gently tap the tube.
- Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.
DNMT1 cDNA Clone Sequence and Quality Control
Sequence:
1 ATGCCAGCGC GTACCGCCCC AGCCCGGGTG CCCACACTGG CCGTCCCGGC CATCTCGCTG 61 CCCGACGATG TCCGCAGGCG GCTCAAAGAT TTGGAAAGAG ACAGCTTAAC AGAAAAGGAA 121 TGTGTGAAGG AGAAATTGAA TCTCTTGCAC GAATTTCTGC AAACAGAAAT AAAGAATCAG 181 TTATGTGACT TGGAAACCAA ATTACGTAAA GAAGAATTAT CCGAGGAGGG CTACCTGGCT 241 AAAGTCAAAT CCCTTTTAAA TAAAGATTTG TCCTTGGAGA ACGGTGCTCA TGCTTACAAC 301 CGGGAAGTGA ATGGACGTCT AGAAAACGGG AACCAAGCAA GAAGTGAAGC CCGTAGAGTG 361 GGAATGGCAG ATGCCAACAG CCCCCCCAAA CCCCTTTCCA AACCTCGCAC GCCCAGGAGG 421 AGCAAGTCCG ATGGAGAGGC TAAGCGTTCA AGAGACCCTC CTGCCTCAGC CTCCCAAGTA 481 ACTGGGATTA GAGCTGAACC TTCACCTAGC CCCAGGATTA CAAGGAAAAG CACCAGGCAA 541 ACCACCATCA CATCTCATTT TGCAAAGGGC CCTGCCAAAC GGAAACCTCA GGAAGAGTCT 601 GAAAGAGCCA AATCGGATGA GTCCATCAAG GAAGAAGACA AAGACCAGGA TGAGAAGAGA 661 CGTAGAGTTA CATCCAGAGA ACGAGTTGCT AGACCGCTTC CTGCAGAAGA ACCTGAAAGA 721 GCAAAATCAG GAACGCGCAC TGAAAAGGAA GAAGAAAGAG ATGAAAAAGA AGAAAAGAGA 781 CTCCGAAGTC AAACCAAAGA ACCAACACCC AAACAGAAAC TGAAGGAGGA GCCGGACAGA 841 GAAGCCAGGG CAGGCGTGCA GGCTGACGAG GACGAAGATG GAGACGAGAA AGATGAGAAG 901 AAGCACAGAA GTCAACCCAA AGATCTAGCT GCCAAACGGA GGCCCGAAGA AAAAGAACCT 961 GAAAAAGTAA ATCCACAGGT TTCTGATGAA AAAGACGAGG ATGAAAAGGA GGAGAAGAGA 1021 CGCAAAACGA CCCCCAAAGA ACCAACGGAG AAAAAAATGG CTCGCGCCAA AACAGTCATG 1081 AACTCCAAGA CCCACCCTCC CAAGTGCATT CAGTGCGGGC AGTACCTGGA CGACCCTGAC 1141 CTCAAATATG GGCAGCACCC ACCAGACGCG GTGGATGAGC CACAGATGCT GACAAATGAG 1201 AAGCTGTCCA TCTTTGATGC CAACGAGTCT GGCTTTGAGA GTTATGAGGC GCTTCCCCAG 1261 CACAAACTGA CCTGCTTCAG TGTGTACTGT AAGCACGGTC ACCTGTGTCC CATCGACACC 1321 GGCCTCATCG AGAAGAATAT CGAACTCTTC TTTTCTGGTT CAGCAAAACC AATCTATGAT 1381 GATGACCCGT CTCTTGAAGG TGGTGTTAAT GGCAAAAATC TTGGCCCCAT AAATGAATGG 1441 TGGATCACTG GCTTTGATGG AGGTGAAAAG GCCCTCATCG GCTTCAGCAC CTCATTTGCC 1501 GAATACATTC TGATGGATCC CAGTCCCGAG TATGCGCCCA TATTTGGGCT GATGCAGGAG 1561 AAGATCTACA TCAGCAAGAT TGTGGTGGAG TTCCTGCAGA GCAATTCCGA CTCGACCTAT 1621 GAGGACCTGA TCAACAAGAT CGAGACCACG GTTCCTCCTT CTGGCCTCAA CTTGAACCGC 1681 TTCACAGAGG ACTCCCTCCT GCGACACGCG CAGTTTGTGG TGGAGCAGGT GGAGAGTTAT 1741 GACGAGGCCG GGGACAGTGA TGAGCAGCCC ATCTTCCTGA CGCCCTGCAT GCGGGACCTG 1801 ATCAAGCTGG CTGGGGTCAC GCTGGGACAG AGGCGAGCCC AGGCGAGGCG GCAGACCATC 1861 AGGCATTCTA CCAGGGAGAA GGACAGGGGA CCCACGAAAG CCACCACCAC CAAGCTGGTC 1921 TACCAGATCT TCGATACTTT CTTCGCAGAG CAAATTGAAA AGGATGACAG AGAAGACAAG 1981 GAGAACGCCT TTAAGCGCCG GCGATGTGGC GTCTGTGAGG TGTGTCAGCA GCCTGAGTGT 2041 GGGAAATGTA AAGCCTGCAA GGACATGGTT AAATTTGGTG GCAGTGGACG GAGCAAGCAG 2101 GCTTGCCAAG AGCGGAGGTG TCCCAATATG GCCATGAAGG AGGCAGATGA CGATGAGGAA 2161 GTCGATGATA ACATCCCAGA GATGCCGTCA CCCAAAAAAA TGCACCAGGG GAAGAAGAAG 2221 AAACAGAACA AGAATCGCAT CTCTTGGGTC GGAGAAGCCG TCAAGACTGA TGGGAAGAAG 2281 AGTTACTATA AGAAGGTGTG CATTGATGCG GAAACCCTGG AAGTGGGGGA CTGTGTCTCT 2341 GTTATTCCAG ATGATTCCTC AAAACCGCTG TATCTAGCAA GGGTCACGGC GCTGTGGGAG 2401 GACAGCAGCA ACGGGCAGAT GTTTCACGCC CACTGGTTCT GCGCTGGGAC AGACACAGTC 2461 CTCGGGGCCA CGTCGGACCC TCTGGAGCTG TTCTTGGTGG ATGAATGTGA GGACATGCAG 2521 CTTTCATATA TCCACAGCAA AGTGAAAGTC ATCTACAAAG CCCCCTCCGA AAACTGGGCC 2581 ATGGAGGGAG GCATGGATCC CGAGTCCCTG CTGGAGGGGG ACGACGGGAA GACCTACTTC 2641 TACCAGCTGT GGTATGATCA AGACTACGCG AGATTCGAGT CCCCTCCAAA AACCCAGCCA 2701 ACAGAGGACA ACAAGTTCAA ATTCTGTGTG AGCTGTGCCC GTCTGGCTGA GATGAGGCAA 2761 AAAGAAATCC CCAGGGTCCT GGAGCAGCTC GAGGACCTGG ATAGCCGGGT CCTCTACTAC 2821 TCAGCCACCA AGAACGGCAT CCTGTACCGA GTTGGTGATG GTGTGTACCT GCCCCCTGAG 2881 GCCTTCACGT TCAACATCAA GCTGTCCAGT CCCGTGAAAC GCCCACGGAA GGAGCCCGTG 2941 GATGAGGACC TGTACCCAGA GCACTACCGG AAATACTCCG ACTACATCAA AGGCAGCAAC 3001 CTGGATGCCC CTGAGCCCTA CCGAATTGGC CGGATCAAAG AGATCTTCTG TCCCAAGAAG 3061 AGCAACGGCA GGCCCAATGA GACTGACATC AAAATCCGGG TCAACAAGTT CTACAGGCCT 3121 GAGAACACCC ACAAGTCCAC TCCAGCGAGC TACCACGCAG ACATCAACCT GCTCTACTGG 3181 AGCGACGAGG AGGCCGTGGT GGACTTCAAG GCTGTGCAGG GCCGCTGCAC CGTGGAGTAT 3241 GGGGAGGACC TGCCCGAGTG CGTCCAGGTG TACTCCATGG GCGGCCCCAA CCGCTTCTAC 3301 TTCCTCGAGG CCTATAATGC AAAGAGCAAA AGCTTTGAAG ATCCTCCCAA CCATGTCCGT 3361 AGCCCTGGAA ACAAAGGGAA GGGCAAGGGA AAAGGGAAGG GCAAGCCCAA GTCCCAAGCC 3421 TGTGAGCCGA GCGAGCCAGA GATAGAGATC AAGCTGCCCA AGCTGCGGAC CCTGGATGTG 3481 TTTTCTGGCT GCGGGGGGTT GTCGGAGGGA TTCCACCAAG CAGGCATCTC TGACACGCTG 3541 TGGGCCATCG AGATGTGGGA CCCTGCGGCC CAGGCGTTCC GGCTGAACAA CCCCGGCTCC 3601 ACAGTGTTCA CAGAGGACTG CAACATCCTG CTGAAGCTGG TCATGGCTGG GGAGACCACC 3661 AACTCCCGCG GCCAGCGGCT GCCCCAGAAG GGAGACGTGG AGATGCTGTG CGGCGGGCCG 3721 CCCTGCCAGG GCTTCAGCGG CATGAACCGC TTCAATTCGC GCACCTACTC CAAGTTCAAA 3781 AACTCTCTGG TGGTTTCCTT CCTCAGCTAC TGCGACTACT ACCGGCCCCG GTTCTTCCTC 3841 CTGGAGAATG TCAGGAACTT TGTCTCCTTC AAGCGCTCCA TGGTCCTGAA GCTCACCCTC 3901 CGCTGCCTGG TCCGCATGGG CTATCAGTGC ACCTTCGGCG TGCTGCAGGC CGGTCAGTAC 3961 GGCGTGGCCC AGACTAGGAG GCGGGCCATC ATCCTGGCCG CGGCCCCTGG AGAGAAGCTC 4021 CCTCTGTTCC CGGAGCCACT GCACGTGTTT GCTCCCCGGG CCTGCCAGCT GAGCGTGGTG 4081 GTGGATGACA AGAAGTTTGT GAGCAACATA ACCAGGTTGA GCTCGGGTCC TTTCCGGACC 4141 ATCACGGTGC GAGACACGAT GTCCGACCTG CCGGAGGTGC GGAATGGAGC CTCGGCACTG 4201 GAGATCTCCT ACAACGGGGA GCCTCAGTCC TGGTTCCAGA GGCAGCTCCG GGGCGCACAG 4261 TACCAGCCCA TCCTCAGGGA CCACATCTGT AAGGACATGA GTGCATTGGT GGCTGCCCGC 4321 ATGCGGCACA TCCCCTTGGC CCCAGGGTCA GACTGGCGCG ATCTGCCCAA CATCGAGGTG 4381 CGGCTCTCAG ACGGCACCAT GGCCAGGAAG CTGCGGTATA CCCACCATGA CAGGAAGAAC 4441 GGCCGCAGCA GCTCTGGGGC CCTCCGTGGG GTCTGCTCCT GCGTGGAAGC CGGCAAAGCC 4501 TGCGACCCCG CAGCCAGGCA GTTCAACACC CTCATCCCCT GGTGCCTGCC CCACACCGGG 4561 AACCGGCACA ACCACTGGGC TGGCCTCTAT GGAAGGCTCG AGTGGGACGG CTTCTTCAGC 4621 ACAACCGTCA CCAACCCCGA GCCCATGGGC AAGCAGGGCC GCGTGCTCCA CCCAGAGCAG 4681 CACCGTGTGG TGAGCGTGCG GGAGTGTGCC CGCTCCCAGG GCTTCCCTGA CACCTACCGG 4741 CTCTTCGGCA ACATCCTGGA CAAGCACCGG CAGGTGGGCA ATGCCGTGCC ACCGCCCCTG 4801 GCCAAAGCCA TTGGCTTGGA GATCAAGCTT TGTATGTTGG CCAAAGCCCG AGAGAGTGCC 4861 TCAGCTAAAA TAAAGGAGGA GGAAGCTGCT AAGGACTAG
Quality Control:cDNA sequencing results is available upon request.
