Human DNASE1 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13801-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

DNASE1 cDNA Clone Datasheet

DNASE1 cDNA Clone Product Information

Gene Bank Ref.ID:	BC029437
cDNA Size:	849
Gene Synonym:	DKFZp686H0155, DNL1, DRNI, FLJ38093, FLJ44902,DNASE1
Source:	Human

DNASE1 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens deoxyribonuclease I DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

DNASE1 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

DNASE1 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

DNASE1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAGGGGCA TGAAGCTGCT GGGGGCGCTG CTGGCACTGG CGGCCCTACT GCAGGGGGCC
61       GTGTCCCTGA AGATCGCAGC CTTCAACATC CAGACATTTG GGGAGACCAA GATGTCCAAT
121      GCCACCCTCG TCAGCTACAT TGTGCAGATC CTGAGCCGCT ATGACATCGC CCTGGTCCAG
181      GAGGTCAGAG ACAGCCACCT GACTGCCGTG GGGAAGCTGC TGGACAACCT CAATCAGGAT
241      GCACCAGACA CCTATCACTA CGTGGTCAGT GAGCCACTGG GACGGAACAG CTATAAGGAG
301      CGCTACCTGT TCGTGTACAG GCCTGACCAG GTGTCTGCGG TGGACAGCTA CTACTACGAT
361      GATGGCTGCG AGCCCTGCGG GAACGACACC TTCAACCGAG AGCCAGCCAT TGTCAGGTTC
421      TTCTCCCGGT TCACAGAGGT CAGGGAGTTT GCCATTGTTC CCCTGCATGC GGCCCCGGGG
481      GACGCAGTAG CCGAGATCGA CGCTCTCTAT GACGTCTACC TGGATGTCCA AGAGAAATGG
541      GGCTTGGAGG ACGTCATGTT GATGGGCGAC TTCAATGCGG GCTGCAGCTA TGTGAGACCC
601      TCCCAGTGGT CATCCATCCG CCTGTGGACA AGCCCCACCT TCCAGTGGCT GATCCCCGAC
661      AGCGCTGACA CCACAGCTAC ACCCACGCAC TGTGCCTATG ACAGGATCGT GGTTGCAGGG
721      ATGCTGCTCC GAGGCGCCGT TGTTCCCGAC TCGGCTCTTC CCTTTAACTT CCAGGCTGCC
781      TATGGCCTGA GTGACCAACT GGCCCAAGCC ATCAGTGACC ACTATCCAGT GGAGGTGATG
841      CTGAAGTGA

Quality Control:cDNA sequencing results is available upon request.