Human DMWD Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG12289-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

DMWD cDNA Clone Datasheet

DMWD cDNA Clone Product Information

Gene Bank Ref.ID:	BC019266
cDNA Size:	1005
Gene Synonym:	DMRN9; DMR-N9; gene59; D19S593E; DMWD
Source:	Human

DMWD cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens dystrophia myotonica, WD repeat containing DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

DMWD cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

DMWD cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

DMWD cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCTCCTGC GTGGGCTCAT GAAGAGCTAC TTTGGGGGCC TGCTGTGTGT GTGCTGGAGC
61       CCTGACGGCC GCTACGTGGT GACGGGTGGC GAAGATGACC TGGTCACCGT GTGGTCCTTC
121      ACCGAGGGCC GCGTGGTGGC TCGAGGCCAT GGCCACAAGT CCTGGGTCAA CGCTGTGGCC
181      TTTGACCCCT ACACCACAAG GGCAGAGGAG GCGGCGACAG CAGCCGGTGC TGATGGGGAG
241      CGGAGCGGCG AAGAGGAGGA GGAGGAGCCC GAGGCTGCGG GCACAGGCTC GGCCGGGGGC
301      GCCCCGCTCT CTCCACTGCC CAAGGCTGGC TCCATTACTT ACCGCTTTGG CTCGGCGGGC
361      CAGGACACGC AGTTCTGCCT GTGGGACCTC ACTGAAGACG TGCTCTACCC GCACCCGCCC
421      CTGGCCCGCA CCCGCACCCT CCCTGGCACA CCTGGCACCA CGCCACCGGC CGCCAGCAGC
481      TCGAGGGGTG GCGAGCCTGG CCCAGGCCCC CTGCCTCGCT CGCTGTCCCG CTCCAACAGT
541      CTCCCGCACC CAGCTGGCGG GGGCAAGGCG GGCGGCCCGG GTGTGGCGGC AGAGCCTGGC
601      ACACCATTCA GCATTGGCCG CTTCGCCACG CTCACACTGC AGGAGCGGCG GGACCGGGGG
661      GCAGAGAAGG AGCACAAGCG CTACCACAGC CTGGGCAACA TCAGCCGGGG TGGCAGTGGC
721      GGCAGTGGCA GTGGTGGGGA GAAGCCCAGC GGCCCTGTTC CCCGCAGCCG CCTGGACCCC
781      GCCAAGGTGC TGGGCACTGC GCTGTGCCCG CGCATCCACG AGGTGCCCCT GCTGGAGCCC
841      CTTGTGTGCA AGAAGATCGC CCAGGAGCGG CTCACAGTCC TCCTGTTCCT GGAGGACTGC
901      ATCATCACTG CCTGCCAGGA GGGCCTCATC TGCACCTGGG CCCGGCCGGG CAAGGCGGGC
961      ATCTCCTCCC AACCAGGCAA CTCCCCGAGT GGCACAGTGG TGTGA

Quality Control:cDNA sequencing results is available upon request.