Human DMKN Gene cDNA Clone / ORF Clone

DMKN cDNA Clone Datasheet

DMKN cDNA Clone Product Information

• Gene Bank Ref.ID: BC011886
• cDNA Size: 414
• Gene Synonym: UNQ729; ZD52F10; DMKN
• Source: Human

DMKN cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens dermokine DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

DMKN cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

DMKN cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

DMKN cDNA Clone Sequence and Quality Control

Sequence:

1        ATGTTTAACT TTGACACTTT CTGGAAGAAT TTTAAATCCA AGCTGGGTTT CATCAACTGG
61       GATGCCATAA ACAAGAACCA GGTCCCGCCC CCCAGCACCC GAGCCCTCCT CTACTTCAGC
121      CGACTCTGGG AGGATTTCAA ACAGAACACT CCTTTCCTCA ACTGGAAAGC AATTATTGAG
181      GGTGCGGACG CGTCATCACT GCAGAAACGT GCAGGCAGAG CCGATCAGCC GGGTGCAGGA
241      TGGCAGGAGG TGGCAGCTGT AACTTCCAAG AACTACAATT ACAACCAGCA TGCGTATCCC
301      ACTGCCTATG GTGGGAAGTA CTCAGTCAAG ACCCCTGCAA AGGGGGGAGT CTCACCTTCT
361      TCCTCGGCTT CCCGGGTGCA ACCTGGCCTG CTGCAGTGGG TGAAGTTTTG GTAG

 

Quality Control:cDNA sequencing results is available upon request.