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Product Catalog
DLL1 ELISA Pair Set, Human ELISA Pair Set 
| Catalog | Size (Price) | Quantity | In Stock | Operation | Other Information |
| SEK11635 |
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Human DLL1 ELISA Pair Set
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Order or Inquire for DLL1 ELISA Pair Set |
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High affinity Human DLL1 ELISA Pair Set |
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Detection limit - 46.875 pg/ml |
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Affordable price and 30%-80% cost saving for Bulk order |
- Materials
- Protocol
- Trouble Shooting
- Image & Flash
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No signal |
Incorrect or no Detection Antibody was added |
Add appropriate Detection Antibody and continue |
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Substrate solution was not added |
Add substrate solution and continue |
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Incorrect storage condition |
Check if the kit is stored at recommended condition and used before expiration date |
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Poor Standard Curve |
Standard was incompletely reconstituted or was inappropriately stored |
Aliquot reconstituted standard and store at -70 ℃ |
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Imprecise / inaccurate pipetting |
Check / calibrate pipettes |
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Incubations done at inappropriate temperature, timing or agitation |
Follow the general ELISA protocol |
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Background wells were contaminated |
Avoid cross contamination by using the sealer appropriately |
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Poor detection value |
The concentration of antigen in samples was too low |
Enriching samples to increase the concentration of antigen |
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Samples were ineffective |
Check if the samples are stored at cold environment. Detect samples in timely manner |
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High Background |
Insufficient washes |
Use multichannel pipettes without touching the reagents on the plate |
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Increase cycles of washes and soaking time between washes |
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TMB Substrate Solution was contaminated |
TMB Substrate Solution should be clear and colorless prior to addition to wells |
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Materials were contaminated |
Use clean plates, tubes and pipettes tips |
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Non-specificity |
Samples were contaminated |
Avoid cross contamination of samples |
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The concentration of samples was too high |
Try higher dilution rate of samples |
Problems 
Possible Sources 
Solutions 
Human DLL1 ELISA Pair Set Related Products & Topics
Related Areas:
Proteins:
Antibodies:
Background
Delta-like protein 1, also known as Drosophila Delta homolog 1, H-Delta-1, and DLL1, is a single-pass type I membrane protein which contains one DSL domain and eight EGF-like domains. DLL1 is expressed in heart and pancreas, with lower expression in brain and muscle and almost no expression in placenta, lung, liver and kidney. DLL1 acts as a ligand for Notch receptors. DLL1 blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor. The Notch pathway is an evolutionary conserved intercellular signaling pathway involved in numerous biological processes including cell fate determination, cellular differentiation, proliferation, survival, and apoptosis. In mammalian cells, five Notch ligands (Jagged1, 2, DLL1, DLL3, DLL4) and four Notch receptors (Notch 1-4) have been identified, and ligand-receptor interactions results in proteolysis and translocation of the Notch intracellular domain. Analysis of DLL1 mutants reveals that it is necessary and sufficient to maintain a pool of progenitors in the embryonic neuroepithelium. DLL1 serves to prevent the untimely differentiation of neural progenitors. DLL1 also contributes to the control of proliferation and differentiation in (Interfollicular Epidermis) IFE.
