Anti-DLL1 Antibody

Cat: 11635-RP02
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Anti-DLL1 Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-DLL1 Antibody
Validated applications
WB,ELISA
Species reactivity
Reacts with: Human
Specificity
Human DLL1
Immunogen
Recombinant Human DLL1 protein (Catalog#11635-H08H)
Preparation
Produced in rabbits immunized with purified, recombinant Human DLL1 / Delta-1 (rh DLL1; Catalog#11635-H08H; NP_005609.3; Met 1-Gly 540). DLL1 specific IgG was purified by human DLL1 affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-DLL1 Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:1000  
ELISA 1:25000-1:50000  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-DLL1 Antibody (Rabbit Polyclonal antibody) Images
Human DLL1 / Delta-like 1 Western blot (WB) 13430

Anti-DLL1 rabbit polyclonal antibody at 1:500 dilution

Lane A: Hela Membrane Lysate

Lysates/proteins at 20 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:68 kDa

Observed band size:72 kDa

(We are unsure as to the identity of these extra bands.)

Anti-DLL1 Antibody Alternative Names
Anti-Delta Antibody;Anti-DELTA1 Antibody;Anti-DL1 Antibody
DLL1 Background Information

Delta-like protein 1(DLL1), also known as Delta1, a single-pass type I membrane protein which contains one DSL domain and eight EGF-like domains,  acts as a ligand for Notch receptors, and positively regulates T-cell development. DLL1 is proteolytically processed in a similar manner to the Notch receptor, and it has been speculated to participate in bidirectional signaling. The proteolytic processing of DLL1 helps achieve an asymmetry in Notch signaling in initially equivalent myogenic cells and helps sustain the balance between differentiation and self-renewal. Interactions between DLL1 and Notch in trans activate the Notch pathway, whereas DLL1 binding to Notch in cis inhibits Notch signaling. DLL1 undergoes proteolytic processing in its extracellular domain by ADAM10. It had been demonstrated that DLL1 represents a substrate for several other members of the ADAM family. In co-transfected cells, DLL1 is constitutively cleaved by ADAM12, and the N-terminal fragment of DLL1 is released to medium. ADAM12-mediated cleavage of DLL1 is cell density-dependent, takes place in cis orientation, and does not require the presence of the cytoplasmic domain of ADAM12. Full-length DLL1, but not its N- or C-terminal proteolytic fragment, co-immunoprecipitates with ADAM12. By using a Notch reporter construct, we show that DLL1 processing by ADAM12 increases Notch signaling in a cell-autonomous manner. Furthermore, ADAM9 and ADAM17 have the ability to process DLL1. In contrast, ADAM15 does not cleave DLL1, although the two proteins still co-immunoprecipitate with each other. During fetal development, DLL1 is an essential Notch ligand in the vascular endothelium of large arteries to activate Notch1 and maintain arterial identity. DLL1-Notch signaling was required for VEGF receptor expression in fetal arteries.

Full Name
delta-like 1 (Drosophila)
References
  • Dyczynska E, et al. (2007) Proteolytic processing of delta-like 1 by ADAM proteases. J Biol Chem. 282(1): 436-44.
  • Sun D, et al. (2008) The role of Delta-like 1 shedding in muscle cell self-renewal and differentiation. J Cell Sci. 121(Pt 22): 3815-23.
  • Srensen I, et al. (2009) DLL1-mediated Notch activation regulates endothelial identity in mouse fetal arteries. Blood. 113(22): 5680-8.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-DLL1 Antibody Rabbit Polyclonal ELISA 80013-T16
    Anti-DLL1 Antibody Rabbit Monoclonal ELISA 80013-R006
    Anti-DLL1 Antibody Rabbit Polyclonal ELISA 80013-RP02
    Anti-DLL1 Antibody Rabbit Polyclonal ELISA 80013-RP01
    Anti-DLL1 Antibody Rabbit Monoclonal ELISA 50522-R019
    Anti-DLL1 Antibody Rabbit Monoclonal ELISA 50522-R036
    Anti-DLL1 Antibody Rabbit Polyclonal ELISA 50522-RP02
    Anti-DLL1 Antibody Rabbit Polyclonal ELISA 50522-RP01
    Anti-DLL1 Antibody Mouse Monoclonal ELISA(Cap) 11635-MM05
    Anti-DLL1 Antibody Rabbit Monoclonal ELISA 11635-R306
    Anti-DLL1 Antibody Mouse Monoclonal ELISA,ELISA(Det) 11635-MM07
    Anti-DLL1 Antibody Rabbit Polyclonal WB,ELISA 11635-RP01
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