DKK3 Antibody

Dkk3 Antibody Datasheet

Dkk3 Antibody Product Information

• Immunogen: Recombinant Mouse Dkk3 protein (Catalog#50247-M08H)
• Antibody Type: Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
• Clone ID: 019
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Mouse DKK3 (rM DKK3; Catalog#50247-M08H; NP_056629.1; Met 1-Ile 349).

Dkk3 Antibody Usage Guide

• Specificity: Mouse Dkk3
• No cross-reactivity in ELISA with

  Human DKK3

• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Mouse DKK3 in WB. Using a DAB detection system, the detection limit for Mouse DKK3 is approximately 2 ng/lane under non-reducing conditions and reducing conditions.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse DKK3. The detection limit for Mouse DKK3 is approximately 0.0049 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Dkk3 Antibody Background

DKK3, also known as REIC (Reduced Expansion in Immortalized Cells), is a member of the dickkopf-related family consisting of four members which have distinct patterns of expression in adult and embryonic tissues. DKK3 is a secreted protein with two cysteine-rich domains separated by a linker region, and has a wide range of effects on tissue development and morphogenesis through its interactions with the Wnt signaling pathway. Mouse DKK3 is highest expressed in brain, eye and heart. The expression of this gene is down-regulated in human immortalized cells and a variety of tumors, such as hepatoma, lung carcinomas and malignant melanoma. Meanwhile, overexpression of DKK3 results in tumor cell line-specific growth inhibition and apoptosis. Accordingly, DKK3 may function as a suppressor gene of tumor growth and be regarded as a promising potential therapeutic target.

References

1. Krupnik, V.E. et al., 1999, Gene. 238: 301-313.
2. Tsuji, T. et al., 2001, Biochem. Biophys. Res. Commun. 289: 257-263.
3. Hsieh, S.Y. et al., 2004, Oncogene. 23: 9183-9189.
4. Hoang, B.H. et al., 2004, Cancer Res. 64: 2734-2739.
5. Ftouh, S. et al., 2005, J. Neurochem. 94: 520-530.