Dkk3 / REIC Antibody (Antigen Affinity Purified)

Dickkopf-Related Protein 3 ( Dkk3 / REIC ) Antibody ( Antigen Affinity Purified )

Dkk3 / REIC Antibody Product Information

• Immunogen: Recombinant human Dkk3 protein ( Catalog#10203-H08H )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, human cell-derived, recombinant human Dkk3 ( rhDkk3; Catalog#10203-H08H; aa 1 - 350; NP_001018067.1 ). Dkk3 specific IgG was purified by human Dkk3 affinity chromatography.

Dkk3 / REIC Antibody Usage Guide

• Specificity: Human Dkk3 / REIC
• Western blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human DKK3 in WB. Using a DAB detection system, the detection limit for Human DKK3 is approximately 0.5 ng/lane under non-reducing conditions and reducing conditions.
• Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human DKK3. The detection limit for human DKK3 is 0.00245 ng / well.
• Storage: This antibody can be stored at 2℃-8℃ for 1 month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly.
  Avoid repeated freeze-thaw cycles.

Dkk3 / REIC Antibody Background

Dkk3, also known as REIC (Reduced Expansion in Immortalized Cells), is a member of the dickkopf-related family which has distinct patterns of expression in adult and embryonic tissues. Dkk3 is a secreted protein with two cysteine-rich domains separated by a linker region, and has a wide range of effects on tissue development and morphogenesis through its interactions with the Wnt signaling pathway. The expression of Dkk3 gene is down regulated in human immortalized cells and a variety of tumors, such as hepatoma, lung carcinomas and malignant melanoma. Meanwhile, overexpression of Dkk3 results in tumor cell line-specific growth inhibition and apoptosis. Dkk3 may function as a suppressor gene of tumor growth and be regarded as a promising potential therapeutic target. 

References

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3. Tsuji, T. et al., 2001, Biochem. Biophys. Res. Commun. 289: 257-263.
4. Hsieh, S.Y. et al., 2004, Oncogene. 23: 9183-9189.
5. Hoang, B.H. et al., 2004, Cancer Res. 64: 2734-2739.