Anti-DKK1 Antibody

Cat: 10170-R015
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Anti-DKK1 Antibody (Rabbit Monoclonal antibody) General Information
Product name
Anti-DKK1 Antibody
Validated applications
Neutralization
Species reactivity
Reacts with: Human
Specificity
Human DKK1
Has cross-reactivity with Rhesus DKK-1 / Dkk1 (Catalog # 11089-K08H) in ELISA assay
Immunogen
Recombinant Human DKK-1 Protein (Catalog#10170-H08H)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human DKK-1 (rh DKK-1; Catalog#10170-H08H; NP_036374.1; Met2-His266) and was produced using recombinant antibody technology.
Source
Monoclonal Rabbit IgG Clone #R015
Purification
Protein A
Formulation
0.2 μm filtered solution in Histidine and Arginine buffer containing 120mM NaCl, 0.02% Tween 80, pH6.0
Conjugate
Unconjugated
Endotoxin
< 3 EU/mg
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-DKK1 Antibody (Rabbit Monoclonal antibody) Validated Applications
Application Notes
Neutralization The neutralization activity of antibody is Measured by its ability to inhibit Wnt3a-induced alkaline phosphatase production by C3H10T1/2 cells. The Neutralization titer (IC50) is typically 0.1-0.4 μg/mL in the presence of 40 ng/mL Mouse Wnt-3a and 10 μg/mL DKK1 (Catalog # 10170-H08H) .

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-DKK1 Antibody Alternative Names
Anti-DKK-1 Antibody;Anti-SK Antibody
DKK1 Background Information

Dickkopf (DKK) family proteins, consisting of DKK-1, DKK-2, DKK-3 and DKK-4, function as secreted Wnt antagonists by inhibiting Wnt coreceptors LRP5/6. DKK-1, DKK-2, and DKK-4 also bind cell surface Kremen-1 or Kremen-2 and promote the internalization of LRP5/6. Dickkopf related protein 1 (DKK-1) was initially identified as an inducer of head formation in Xenopus embryos. DKK-1 protein modulates Wnt signaling pathway during embryonic development. Increased levels of DKK-1 are found in the majority of lung cancers, esophageal squamous cell carcinomas, and hormone-resistant breast cancers, while DKK-1 expression is decreased in malignant melanoma and colorectal cancers.

Full Name
dickkopf WNT signaling pathway inhibitor 1
References
  • Horwitz EM. (2004) Dkk-1-mediated expansion of adult stem cells. Trends Biotechnol. 22(8): 386-8.
  • Jiang T, et al. (2009) Clinical significance of serum DKK-1 in patients with gynecological cancer. Int J Gynecol Cancer. 19(7): 1177-81.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-DKK1 Antibody Rabbit Polyclonal ELISA 11089-T16
    Anti-DKK1 Antibody Rabbit Polyclonal ELISA 11089-RP01
    Anti-DKK1 Antibody Rabbit Polyclonal WB,ELISA 11089-T48
    Anti-DKK1 Antibody Rabbit Polyclonal WB,ELISA 10170-RP01
    Anti-DKK1 Antibody Rabbit Polyclonal ELISA,IHC-P 10170-T16
    Anti-DKK1 Antibody Rabbit Polyclonal WB,ELISA,IP 10170-RP02
    Anti-DKK1 Antibody Rabbit Monoclonal WB,ELISA 10170-R004
    Anti-DKK1 Antibody Mouse Monoclonal ELISA,ELISA(Det) 10170-MM03
    Anti-DKK1 Antibody Mouse Monoclonal ELISA(Cap) 10170-MM08
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