|Neutralization||The neutralization activity of antibody is Measured by its ability to inhibit Wnt3a-induced alkaline phosphatase production by C3H10T1/2 cells. The Neutralization titer (IC50) is typically 0.1-0.4 μg/mL in the presence of 40 ng/mL Mouse Wnt-3a and 10 μg/mL DKK1 (Catalog # 10170-H08H) .|
**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********
Dickkopf (DKK) family proteins, consisting of DKK-1, DKK-2, DKK-3 and DKK-4, function as secreted Wnt antagonists by inhibiting Wnt coreceptors LRP5/6. DKK-1, DKK-2, and DKK-4 also bind cell surface Kremen-1 or Kremen-2 and promote the internalization of LRP5/6. Dickkopf related protein 1 (DKK-1) was initially identified as an inducer of head formation in Xenopus embryos. DKK-1 protein modulates Wnt signaling pathway during embryonic development. Increased levels of DKK-1 are found in the majority of lung cancers, esophageal squamous cell carcinomas, and hormone-resistant breast cancers, while DKK-1 expression is decreased in malignant melanoma and colorectal cancers.
|Product Description||Host||Clonality||Application||Catalog# (PDF)|