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DKK-1 Antibody (Antigen Affinity Purified) Datasheet
|Order or Inquire for DKK-1 Antibody product||Quality antibodies||Antibody production services|
|Detection limit is 2 ng/lane in WB|
|Detection limit is 0.00975 ng/well in ELISA|
DKK-1 Antibody Product Information
Recombinant Human DKK-1 protein (Catalog #10170-H08H)
|Antibody Type :||Rabbit Polyclonal Antibody ( Antibody Purification Platform )|
|Ig Type :||
|Formulation :||0.2 μm filtered solution in PBS with 5% trehalose|
Produced in rabbits immunized with purified, human cell-derived, recombinant Human DKK-1 (rh DKK-1; Catalog #10170-H08H; NP_036374.1; Met 2-His 266). DKK-1 specific IgG was purified by Human DKK-1 affinity chromatography.
DKK-1 Antibody Usage Guide
|Western blot :||This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human DKK1 in WB. Using a DAB detection system, the detection limit for Human DKK1 is approximately 2 ng/lane under non-reducing conditions and reducing conditions.|
|Direct ELISA :||This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human DKK1. The detection limit for Human DKK1 is approximately 0.00975 ng/well.|
|Storage :||This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
DKK-1 Antibody Related Products & Topics
DKK-1 Antibody Background
- Krupnik, V.E. et al., 1999, Gene. 238: 301.
- Fedi, P. et al., 1999, J. Biol. Chem. 274: 19465-19472.
- Glinka, A. et al., 1998, Nature. 391: 357-362.
- Mao, B. et al., 2001, Nature. 411: 321-325.
- Semenov, M.V. et al., 2001, Curr. Biol. 11: 951-961.
- Qiang, Y.M. et al., 2008, Blood. 112: 196-207.