Anti-DKK1 Antibody

Cat: 10170-RP02
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Anti-DKK1 Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-DKK1 Antibody
Validated applications
WB,ELISA,IP
Species reactivity
Reacts with: Human
Specificity
Human DKK1
Immunogen
Recombinant Human DKK-1 protein (Catalog#10170-H08H)
Preparation
Produced in rabbits immunized with purified, recombinant Human DKK-1 (rh DKK-1; Catalog#10170-H08H; NP_036374.1; Met 2-His 266). DKK-1 specific IgG was purified by Human DKK-1 affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-DKK1 Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:2000  
ELISA 1:5000-1:10000  
IP 4-6 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-DKK1 Antibody (Rabbit Polyclonal antibody) Images
Human DKK1/Dkk-1 Immunoprecipitation(IP) 7952

DKK1 was immunoprecipitated using:

Lane A:0.5 mg Hela Whole Cell Lysate

Lane B:0.5 mg HepG2 Whole Cell Lysate

2 µL anti-DKK1 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-DKK1 rabbit polyclonal antibody,at 1:200 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 28 kDa

Observed band size: 28 kDa

Human DKK1/Dkk-1 Western blot (WB) 13135

Anti-DKK1 rabbit polyclonal antibody at 1:500 dilution

Lane A: HepG2 Whole Cell Lysate

Lane B: HeLa Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:38 kDa

Observed band size:36 kDa

Anti-DKK1 Antibody Alternative Names
Anti-DKK-1 Antibody;Anti-SK Antibody
DKK1 Background Information

Dickkopf (DKK) family proteins, consisting of DKK-1, DKK-2, DKK-3 and DKK-4, function as secreted Wnt antagonists by inhibiting Wnt coreceptors LRP5/6. DKK-1, DKK-2, and DKK-4 also bind cell surface Kremen-1 or Kremen-2 and promote the internalization of LRP5/6. Dickkopf related protein 1 (DKK-1) was initially identified as an inducer of head formation in Xenopus embryos. DKK-1 protein modulates Wnt signaling pathway during embryonic development. Increased levels of DKK-1 are found in the majority of lung cancers, esophageal squamous cell carcinomas, and hormone-resistant breast cancers, while DKK-1 expression is decreased in malignant melanoma and colorectal cancers.

Full Name
dickkopf WNT signaling pathway inhibitor 1
References
  • Horwitz EM. (2004) Dkk-1-mediated expansion of adult stem cells. Trends Biotechnol. 22(8): 386-8.
  • Jiang T, et al. (2009) Clinical significance of serum DKK-1 in patients with gynecological cancer. Int J Gynecol Cancer. 19(7): 1177-81.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-DKK1 Antibody Rabbit Polyclonal ELISA 11089-T16
    Anti-DKK1 Antibody Rabbit Polyclonal ELISA 11089-RP01
    Anti-DKK1 Antibody Rabbit Polyclonal WB,ELISA 11089-T48
    Anti-DKK1 Antibody Rabbit Polyclonal WB,ELISA 10170-RP01
    Anti-DKK1 Antibody Rabbit Polyclonal ELISA,IHC-P 10170-T16
    Anti-DKK1 Antibody Rabbit Monoclonal Neutralization 10170-R015
    Anti-DKK1 Antibody Rabbit Monoclonal WB,ELISA 10170-R004
    Anti-DKK1 Antibody Mouse Monoclonal ELISA,ELISA(Det) 10170-MM03
    Anti-DKK1 Antibody Mouse Monoclonal ELISA(Cap) 10170-MM08
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