DKK-1 Antibody (Antigen Affinity Purified)

DKK-1 Antibody (Antigen Affinity Purified) Datasheet

DKK-1 Antibody Product Information

• Immunogen: Recombinant Human DKK-1 protein (Catalog #10170-H08H)
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, human cell-derived, recombinant Human DKK-1 (rh DKK-1; Catalog #10170-H08H; NP_036374.1; Met 2-His 266). DKK-1 specific IgG was purified by Human DKK-1 affinity chromatography.

DKK-1 Antibody Usage Guide

• Specificity: Human DKK-1
• Western blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human DKK1 in WB. Using a DAB detection system, the detection limit for Human DKK1 is approximately 2 ng/lane under non-reducing conditions and reducing conditions.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human DKK1. The detection limit for Human DKK1 is approximately 0.00975 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

DKK-1 Antibody Background

DKK1 (dickkopf related protein 1) is a head inducer secreted from the vertebrate head organizer and belongs to the DKK protein family including four members. DKK1 is synthesized initially as a precursor protein with an N-terminal signal peptide and two cysteine-rich domains. DKK proteins have distinct patterns of expression in adult and embryonic tissues and have effects on Wnt signaling implicated in diverse processes. Dkk1 is a specific and high-affinity ligand for LRP6 (LDL-receptor-related protein 6) acting as a Wnt co-receptor. Dkk1 blocks LRP6-mediated Wnt/beta-catenin signaling by antagonistically inhibiting the formation of Wnt-Fz-LRP6 complex which triggers the Wnt signal pathway. It is revealed that Wnt signaling in osteoblasts is necessary to promote BMP-2-mediated differentiation of pre-osteoblast cells, and therefore, DKK1 may play a key role in regulating pre-osteoblast differentiation and the development of multiple myeloma-associated osteolytic bone lesions.

References

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