Human DIXDC1 Gene cDNA Clone / ORF Clone

Human DIXDC1 cDNA Clone Datasheet

DIXDC1 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_001037954.2
• cDNA Size: 2052
• Gene Synonym: CCD1; KIAA1735; DIXDC1
• Source: Human

DIXDC1 cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens DIX domain containing 1 DNA.
• Vector: pGEM-T Vector

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 1102 C/T, resulting in the amino acid Thr substitution by Ile.

DIXDC1 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

DIXDC1 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

DIXDC1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCTAGCCT GCCTGACCCG AGGGAACTTA CTGGACGTCC TGCAGGAGGG CTTCAATGAG
61       CAACAGCTGC AGGCCTATGT GGCCTGGGTG AATGCACAGC TGAAGAAGAG GCCAGCAGTG
121      AAGCCTGTGC AGGACCTGCG ACAAGATCTC CGGGATGGGG TGATCCTGGC ATATCTCATC
181      GAGATTGTTG CAGGAGAAAA GCTGAGTGGG GTACAGCTGA GTCCCGGTAA CCAACAGGAG
241      ATGAAGAATA ATGTGGAGAA AGTGCTACAG TTTGTGGCCT CTAAAAAGAT TCGTATGCAC
301      CAGACTTCGG CTAAAGATAT TGTGGATGGA AACCTGAAGT CTATCATGAG GCTGGTCCTT
361      GCCTTGGCAG CTCATTTCAA ACCTGGCTCC AGCAGGACGG TGAACCAAGG ACGGGACTCC
421      AGAGCCCCTC TGCAAAGTCA CCGACCACAC TGTGCCACTG CTGTTGCCCA GGGAGCAGCT
481      GCTGCTCTGG CCGATGTGTG TCATGACATG TCCCGATCAG GACGGGATGT CTTTCGATAT
541      AGACAGAGGA ACAGCAGCAT GGATGAGGAA ATTGAGAATC CATACTGGAG TGTGCGGGCC
601      CTAGTGCAGC AGTACGAAGG GCAACAAAGG TCCCCGTCTG AATCCAGCTG CTCCAGCCTG
661      ACTTCACCCA GTCCAATCCA CAGTGCAAAG AGCGAGTCCA TTATAACCCA GTCAGAAGAG
721      AAGGCAGATT TTGTGATTAT TCCCGCTGAA GGAATAGAGA ACAGAACAGA AGGGACAGAT
781      TCTCCATTAT CTCGAGACTG GCGGCCAGGG AGCCCTGGAA CCTATCTGGA GACCTCATGG
841      GAAGAACAGC TGTTGGAACA ACAAGAATAT TTAGAAAAAG AAATGGAGGA AGCAAAGAAA
901      ATGATATCAG GACTACAGGC CTTACTGCTC AATGGATCCT TACCTGAAGA TGAACAGGAG
961      AGGCCCTTGG CCCTCTGTGA ACCAGGTGTC AATCCCGAGG AACAACTGAT TATAATCCAA
1021     AGTCGTCTGG ATCAGAGTAT GGAGGAGAAT CAGGACTTAA AGAAGGAACT GCTGAAATGT
1081     AAACAAGAAG CCAGAAACTT ATAGGGGATA AAGGATGCCT TGCAGCAGAG ATTGACTCAG
1141     CAGGACACAT CTGTTCTTCA GCTCAAACAA GAGCTACTGA GGGCAAATAT GGACAAAGAT
1201     GAGCTGCACA ACCAGAATGT GGATCTGCAG AGGAAGCTAG ATGAGAGGAA CCGGCTCTTG
1261     GGAGAATATA AAAAAGAGCT GGGGCAGAAG GATCGCCTTC TTCAGCAGCA CCAGGCCAAG
1321     TTAGAAGAAG CACTCCGGAA ACTCTCTGAT GTCAGTTACC ACCAGGTGGA TCTAGAGCGA
1381     GAGCTAGAAC ACAAAGATGT CCTCTTGGCT CACTGTATGA AAAGAGAGGC AGATGAGGCG
1441     ACCAACTACA ACAGTCACAA CTCTCAAAGC AATGGTTTTC TCCTTCCAAC GGCAGGAAAA
1501     GGAGCTACTT CAGTCAGCAA CAGAGGGACC AGCGACCTGC AGCTTGTTCG AGATGCTCTC
1561     CGCAGCCTGC GCAACAGCTT CAGTGGCCAC GATCCTCAGC ACCACACTAT TGACAGCTTG
1621     GAGCAGGGCA TTTCTAGCCT CATGGAGCGC CTGCATGTTA TGGAGACGCA GAAGAAACAA
1681     GAAAGAAAGG TTCGGGTCAA GTCACCCAGA ACTCAAGTAG GTAGTGAATA CCGGGAGTCC
1741     TGGCCCCCTA ACTCAAAGTT GCCTCACTCA CAGAGCTCTC CAACTGTCAG CAGCACCTGT
1801     ACTAAAGTGC TCTATTTCAC TGACCGGTCA CTTACGCCCT TCATGGTCAA TATACCAAAG
1861     AGGTTGGAGG AGGTGACGTT AAAGGATTTT AAAGCAGCTA TTGATCGGGA AGGAAATCAC
1921     CGGTATCACT TCAAAGCACT GGATCCTGAG TTTGGCACTG TCAAAGAGGA GATTTTCCAT
1981     GATGATGATG CCATCCCTGG ATGGGAAGGG AAAATTGTAG CTTGGGTGGA AGAAGACCAT
2041     GGAGAGAATT AA

 

Quality Control:cDNA sequencing results is available upon request.