Human DFFA Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG12152-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

DFFA cDNA Clone Datasheet

DFFA cDNA Clone Product Information

Gene Bank Ref.ID:	BC007721
cDNA Size:	996
Gene Synonym:	DFF1; ICAD; DFF-45;DFFA
Source:	Human

DFFA cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens DNA fragmentation factor, 45kDa, alpha polypeptide DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

DFFA cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

DFFA cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

DFFA cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGAGGTGA CCGGGGACGC CGGGGTACCA GAATCTGGCG AGATCCGGAC TCTAAAGCCG
61       TGTCTGCTGC GCCGCAACTA CAGCCGCGAA CAGCACGGCG TGGCCGCCTC CTGCCTCGAA
121      GACCTGAGGA GCAAGGCCTG TGACATTCTG GCCATTGATA AGTCCCTGAC ACCAGTCACC
181      CTGGTCCTGG CAGAGGATGG CACCATAGTG GATGATGACG ATTACTTTCT GTGTCTACCT
241      TCCAATACTA AGTTTGTGGC ATTGGCTAGT AATGAGAAAT GGGCATACAA CAATTCAGAT
301      GGAGGTACAG CTTGGATTTC CCAAGAGTCC TTTGATGTAG ATGAAACAGA CAGCGGGGCA
361      GGGTTGAAGT GGAAGAATGT GGCCAGGCAG CTGAAAGAAG ATCTGTCCAG CATCATCCTC
421      CTATCAGAGG AGGACCTCCA GATGCTTGTT GACGCTCCCT GCTCAGACCT GGCTCAGGAA
481      CTACGTCAGA GTTGTGCCAC CGTCCAGCGG CTGCAGCACA CACTCCAACA GGTGCTTGAC
541      CAAAGAGAGG AAGTGCGTCA GTCCAAGCAG CTCCTGCAGC TGTACCTCCA GGCTTTGGAG
601      AAAGAGGGCA GCCTCTTGTC AAAGCAGGAA GAGTCCAAAG CTGCCTTTGG TGAGGAGGTG
661      GATGCAGTAG ACACGGGTAT CAGCAGAGAG ACCTCCTCGG ACGTTGCGCT GGCGAGCCAC
721      ATCCTTACTG CACTGAGGGA GAAGCAGGCT CCAGAGCTGA GCTTATCTAG TCAGGATTTG
781      GAGTTGGTTA CCAAGGAAGA CCCCAAAGCA CTGGCTGTTG CCTTGAACTG GGACATAAAG
841      AAGACGGAGA CTGTTCAGGA GGCCTGTGAG TGGGAGCTCG CCCTGCGCCT GCAGCAGACG
901      CAGAGCTTGC ATTCTCTCCG GAGCATCTCA GCAAGCAAGG CCTCACCACC TGGTGACCTG
961      CAGAATCCTA AGCGAGCCAG ACAGGATCCC ACATAG

Quality Control:cDNA sequencing results is available upon request.