DEFB103A / DEFB103 / Defensin Protein (His Tag)

Defensin, beta 103A Protein Datasheet

DEFB103A / DEFB103 / Defensin Protein Product Information

• Synonym: BD3, DEFB103, DEFB3, DEFB103A
• Protein Construction: A DNA sequence encoding the mature form of human DEFB103A (P81534) (Arg100-Leu233) was expressed, with a polyhistidine tag at the N-terminus.
• Source: Human
• Expression Host: E.coli

DEFB103A / DEFB103 / Defensin Protein QC Testing

• Purity: > 99 % as determined by SDS-PAGE
• Endotoxin: Please contact us for more information.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant human DEFB103A consists of 61 amino acids and predicts a molecular mass of 7.3 KDa. It migrates as an approximately 12 KDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile 50mM Tris, 0.3%Ttiton X-100, 0.3%SKL; pH 8.5.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: DEFB103A / DEFB103 / Defensin protein

DEFB103A / DEFB103 / Defensin Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

DEFB103A / DEFB103 / Defensin Protein Description

Defensin, also known as beta-defensin 3 and DEFB103, is a member of the defensin family which exhibits antimicrobial activity against Gram-positive bacteria S.aureus and S.pyogenes, Gram-negative bacteria P.aeruginosa and E.coli and the yeast C.albicans. Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Members of the defensin family are highly similar in protein sequence. Defensin has broad spectrum antimicrobial activity and may play an important role in innate epithelial defense. Defensin kills multiresistant S.aureus and vancomycin-resistent E.faecium. It has no significant hemolytic activity. Beta-defensin 3 is abundantly expressed in skin and tonsils, and to a lesser extent in trachea, uterus, kidney, thymus, adenoid, pharynx and tongue. It is also expressed in salivary gland, bone marrow, colon, stomach, polyp and larynx. However, in small intestine, it cannot be detected.

References

1. García JR. et al., 2002, Cell Tissue Res. 306 (2): 257-64.
2. Dunsche A. et al., 2002, Eur J Oral Sci. 110 (2): 121-4.
3. Abiko Y. et al., 2004, J Dermatol Sci. 31 (3): 225-8.