|Datasheet||Specific References||Reviews||Related Products||Protocols|
The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.
The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.
|Rat DDR2 Kinase / CD167b Gene cDNA Clone (full-length ORF Clone), expression ready, FLAG-tagged||RG80341-G-F|
|Rat DDR2 Kinase / CD167b Gene cDNA Clone (full-length ORF Clone), expression ready, His-tagged||RG80341-G-H|
|Rat DDR2 Kinase / CD167b Gene cDNA Clone (full-length ORF Clone), expression ready, Myc-tagged||RG80341-G-M|
|Rat DDR2 Kinase / CD167b Gene cDNA Clone (full-length ORF Clone), expression ready, untagged||RG80341-G-N|
|Rat DDR2 Kinase / CD167b Gene cDNA Clone (full-length ORF Clone), expression ready, HA-tagged||RG80341-G-Y|
Discoidin domain receptor 2 (DDR2) or CD167b (cluster of differentiation 167b) is a kind of protein tyrosine kinases associated with cell proliferation and tumor metastasis, and collagen, identified as a ligand for DDR2, up-regulates matrix metallloproteinase 1 (MMP-1) and MMP-2 expression in cellular matrix. DDR2/CD167b was found to recognise the triple-helical region of collagen X as well as the NC1 domain. Binding to the collagenous region was dependent on the triple-helical conformation. DDR2/CD167b autophosphorylation was induced by the collagen X triple-helical region but not the NC1 domain, indicating that the triple-helical region of collagen X contains a specific DDR2 binding site that is capable of receptor activation. DDR2/CD167b is induced during stellate cell activation and implicate the phosphorylated receptor as a mediator of MMP-2 release and growth stimulation in response to type I collagen. Moreover, type I collagen-dependent upregulation of DDR2/CD167b expression establishes a positive feedback loop in activated stellate cells, leading to further proliferation and enhanced invasive activity.