Rat DDR2 Kinase/CD167b Gene cDNA Clone / ORF Clone

DDR2 cDNA Clone Datasheet

DDR2 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_031764.3
• cDNA Size: 2565
• Gene Synonym: Tyro10; Ddr2
• Source: Rat

DDR2 cDNA Clone Description

• cDNA Description: ORF Clone of Rattus norvegicus discoidin domain receptor tyrosine kinase 2 DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

DDR2 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

DDR2 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

DDR2 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGATCCTGA TTCCCAGAAT GCCTCTGGTG CTGCTGCTGC TCCTGCTCAT CCTGGGTTCT
61       GCAAAAGCTC AGGTTAATCC AGCCATATGT CGCTATCCTC TGGGCATGTC AGGAGGCCAC
121      ATTCCAGATG AGGACATCAC AGCTTCAAGT CAGTGGTCAG AATCCACAGC TGCCAAATAT
181      GGGAGGCTGG ACTCTGAAGA AGGGGATGGA GCTTGGTGCC CTGAGATTCC AGTGCAACCC
241      GATGACCTGA AGGAATTTCT GCAGATTGAT TTACGAACCC TACACTTTAT CACTCTTGTG
301      GGGACCCAGG GACGCCATGC AGGGGGTCAT GGCATTGAAT TTGCACCCAT GTACAAGATC
361      AACTACAGTC GGGATGGCAA CCGCTGGATC TCCTGGCGTA ACCGACATGG GAAACAGGTG
421      CTTGATGGGA ACAGTAACCC TTATGATGTT TTCCTGAAGG ATTTGGAGCC ACCCATTGTA
481      GCTAGATTTG TTCGCCTTAT CCCAGTCACT GACCACTCCA TGAACGTGTG CATGAGGGTC
541      GAGCTTTATG GTTGTGTCTG GCTAGATGGC TTGGTATCTT ACAATGCACC CGCTGGGCAG
601      CAGTTTGTAC TCCCTGGAGG CTCCATCATT TATCTGAATG ATTCTGTCTA TGATGGAGCT
661      GTTGGGTACA GTATGACTGA AGGGCTAGGC CAGTTGACTG ATGGGGTATC TGGCCTGGAT
721      GATTTTACCC AGACCCATGA ATACCATGTG TGGCCCGGCT ATGACTACGT GGGATGGCGG
781      AATGAGAGTG CTACCAATGG TTTCATAGAG ATCATGTTTG AATTTGACCG AGTCAGGAAT
841      TTTACCACCA TGAAGGTCCA CTGCAATAAC ATGTTCGCTA AAGGTGTGAA GATCTTTAAG
901      GAGGTCCAAT GTTACTTCCG CTCTGAAACC AGCGAATGGG AACCTACTGC TGTCTACTTT
961      CCCCTTGTCC TGGATGATGT GAACCCCAGT GCTCGGTTTG TCACGGTGCC CCTCCACCAT
1021     CGAATGGCTA GTGCCATCAA ATGCCAATAC CATTTTGCCG ACACATGGAT GATGTTCAGC
1081     GAGATCACTT TTCAATCAGA TGCTGCAATG TATAATAACT CTGGAGCACT CCCCACCTCT
1141     CCTATGGCAC CCACAACCTT TGATCCCATG CTTAAAGTCG ATGACAGCAA CACTCGGATC
1201     CTGATTGGCT GCTTGGTGGC CATCATCTTC ATCCTGCTGG CTGTCATCGT CATCATCCTG
1261     TGGAGGCAAT TCTGGCAGAA GATGCTAGAG AAGGCTTCAC GGAGGATGCT GGATGATGAA
1321     ATGACAGTTA GCCTTTCCCT GCCCAGCGAG TCCAGCATGT TCAATAACAA CCACTCCTCA
1381     TCACCAAGTG AACAGGAGTC CAACTCTACT TATGATCGAA TCTTTCCCCT TCGCCCTGAC
1441     TACCAGGAGC CATCCAGACT GATCCGAAAG CTCCCAGAAT TTGCTCCAGG AGAGGAAGAG
1501     TCTGGTTGCA GTGGTGTTGT GAAGCCGGCC CAGCCCAATG GACCTGAGGG CGTGCCCCAC
1561     TATGCAGAAG CCGATATAGT GAATCTCCAG GGAGTAACAG GTGGCAACAC CTACTCTGTG
1621     CCTGCCGTCA CCATGGACCT GCTCTCGGGA AAAGATGTGG CTGTGGAAGA GTTCCCCAGG
1681     AAACTGTTAG CCTTCAAAGA GAAGCTGGGA GAAGGCCAGT TTGGGGAGGT TCATCTCTGT
1741     GAAGTGGAGG GAATGGAAAA ATTCAAAGAC AAAGATTTCG CACTAGACGT CAGTGCTAAC
1801     CAGCCTGTCC TGGTGGCCGT GAAAATGCTT CGAGCAGATG CCAACAAGAA TGCCAGGAAT
1861     GATTTTCTTA AGGAGATAAA GATTATGTCT CGGCTCAAGG ACGCAAACAT CATCCGTCTC
1921     TTAGCTGTGT GCATCACTGA GGACCCGCTC TGCATGATCA CGGAATACAT GGAAAACGGA
1981     GACCTCAATC AGTTTCTTTC TCGCCACGAG CCTCTAAGTT CTGGTTCTAG CAATGCCACA
2041     GTCAGTTACG CCAACCTCAA GTTTATGGCT ACCCAGATTG CCTCTGGGAT GAAGTACCTT
2101     TCATCTCTCA ACTTTGTCCA CCGAGATCTG GCCACACGCA ACTGCTTAGT GGGCAAGAAC
2161     TACACCATCA AGATAGCTGA CTTTGGAATG AGCAGAAACC TGTACAGTGG TGACTACTAC
2221     CGGATCCAGG GCCGGGCAGT GCTCCCCATC CGCTGGATGT CCTGGGAAAG CATCTTGCTG
2281     GGCAAATTCA CCACGGCAAG TGATGTGTGG GCCTTTGGGG TGACTCTGTG GGAGACTTTC
2341     ACCTTTTGCC AGGAGCAGCC CTATTCCCAG CTGTCGGACG AGCAGGTTAT TGAGAATACT
2401     GGAGAGTTCT TCCGAGACCA GGGGAGGCAG ATCTATCTCC CTCAACCAGC CGTTTGCCCC
2461     GACTCTGTGT ATAAGCTGAT GCTCAGTTGC TGGAGAAGAG AAACCAAGCA CCGACCATCC
2521     TTCCAAGAAA TACACCTTCT GCTTCTTCAA CAAGGAGCTG AGTGA

 

Quality Control:cDNA sequencing results is available upon request.