|IP||1-4 μL/mg of lysate|
**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********
DDR1 was immunoprecipitated using:
Lane A:0.5 mg MCF-7 Whole Cell Lysate2 µL anti-DDR1 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.Primary antibody:
Anti-DDR1 rabbit monoclonal antibody,at 1:200 dilutionSecondary antibody:
Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.
Performed under reducing conditions.Predicted band size: 101 kDa
Observed band size: 130 kDa
Anti-DDR1 rabbit monoclonal antibody at 1:500 dilution
Lane A: MCF7 Whole Cell LysateLysates/proteins at 30 μg per lane.
Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.
Performed under reducing conditions.Predicted band size:101 kDa
Observed band size:130 kDa
Discoidin domain receptor family, member 1 (DDR1), also known as or CD167a (cluster of differentiation 167a), and Mammary carcinoma kinase 10 (MCK10), belongs to a subfamily of tyrosine kinase receptors with an extracellular domain homologous to Dictyostellium discoideum protein discoidin 1. Receptor tyrosine kinases play a key role in the communication of cells with their microenvironment. These kinases are involved in the regulation of cell growth, differentiation and metabolism. Expression of DDR1/MCK10/CD167 is restricted to epithelial cells, particularly in the kidney, lung, gastrointestinal tract, and brain. In addition, it has been shown to be significantly overexpressed in several human tumors. DDR1/MCK10/CD167 plays an important role in regulating attachment to collagen, chemotaxis, proliferation, and MMP production in smooth muscle cells. DDR1 functions in a feedforward loop to increase p53 levels and at least some of its effectors. Inhibition of DDR1 function resulted in strikingly increased apoptosis of wild-type p53-containing cells in response to genotoxic stress through a caspase-dependent pathway.
|Product Description||Host||Clonality||Application||Catalog# (PDF)|
|Anti-DDR1 Antibody (APC)||Mouse||Monoclonal||FCM||10730-MM05-A|
|Anti-DDR1 Antibody (PE)||Mouse||Monoclonal||FCM||10730-MM05-P|