Anti-DDR1 Antibody

Cat: 10730-RP02
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Anti-DDR1 Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-DDR1 Antibody
Validated applications
WB,ELISA,IP
Species reactivity
Reacts with: Human
Specificity
Human DDR1
Immunogen
Recombinant Human DDR1 protein (Catalog#10730-H08H)
Preparation
Produced in rabbits immunized with purified, recombinant Human DDR1 / CD167a (rh DDR1; Catalog#10730-H08H; NP_001945.3; Met 1-Thr 416). DDR1 specific IgG was purified by human DDR1 affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-DDR1 Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:1000  
ELISA 1:5000-1:10000  
IP 1-2 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-DDR1 Antibody (Rabbit Polyclonal antibody) Images
Human DDR1/MCK10/CD167 Immunoprecipitation(IP) 8013

DDR1 was immunoprecipitated using:

Lane A:0.5 mg SW480 Whole Cell Lysate

Lane B:0.5 mg MCF-7 Whole Cell Lysate

0.5 µL anti-DDR1 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-DDR1 rabbit polyclonal antibody,at 1:1000 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 101 kDa

Observed band size: 130 kDa

Human DDR1/MCK10/CD167 Western blot (WB) 13268

Anti-DDR1 rabbit polyclonal antibody at 1:500 dilution

Lane A: MCF7 Membrane Lysate

Lysates/proteins at 20 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:101 kDa

Observed band size:130 kDa

Anti-DDR1 Antibody Alternative Names
Anti-CAK Antibody;Anti-CD167 Antibody;Anti-DDR Antibody;Anti-EDDR1 Antibody;Anti-HGK2 Antibody;Anti-MCK10 Antibody;Anti-NEP Antibody;Anti-NTRK4 Antibody;Anti-PTK3 Antibody;Anti-PTK3A Antibody;Anti-RTK6 Antibody;Anti-TRKE Antibody
DDR1 Background Information

Discoidin domain receptor family, member 1 (DDR1), also known as or CD167a (cluster of differentiation 167a), and Mammary carcinoma kinase 10 (MCK10), belongs to a subfamily of tyrosine kinase receptors with an extracellular domain homologous to Dictyostellium discoideum protein discoidin 1. Receptor tyrosine kinases play a key role in the communication of cells with their microenvironment. These kinases are involved in the regulation of cell growth, differentiation and metabolism. Expression of DDR1/MCK10/CD167 is restricted to epithelial cells, particularly in the kidney, lung, gastrointestinal tract, and brain. In addition, it has been shown to be significantly overexpressed in several human tumors. DDR1/MCK10/CD167 plays an important role in regulating attachment to collagen, chemotaxis, proliferation, and MMP production in smooth muscle cells. DDR1 functions in a feedforward loop to increase p53 levels and at least some of its effectors. Inhibition of DDR1 function resulted in strikingly increased apoptosis of wild-type p53-containing cells in response to genotoxic stress through a caspase-dependent pathway.

Full Name
discoidin domain receptor tyrosine kinase 1
References
  • Hou G, et al. (2001) The discoidin domain receptor tyrosine kinase DDR1 in arterial wound repair. J Clin Invest. 107(6): 727-35.
  • Ongusaha PP, et al. (2003) p53 induction and activation of DDR1 kinase counteract p53-mediated apoptosis and influence p53 regulation through a positive feedback loop. EMBO J. 22(6): 1289-301.
  • Jönsson M, et al. (2001) Repression of Wnt-5a impairs DDR1 phosphorylation and modifies adhesion and migration of mammary cells. J Cell Sci. 114(11): 2043-53.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-DDR1 Antibody Rabbit Polyclonal WB,IHC-P 80440-T40
    Anti-DDR1 Antibody Rabbit Monoclonal WB,ELISA,IP 10730-R203
    Anti-DDR1 Antibody Rabbit Monoclonal ELISA(Cap) 10730-R205
    Anti-DDR1 Antibody (APC) Mouse Monoclonal FCM 10730-MM05-A
    Anti-DDR1 Antibody (PE) Mouse Monoclonal FCM 10730-MM05-P
    Anti-DDR1 Antibody Rabbit Polyclonal ELISA 10730-RP01
    Anti-DDR1 Antibody Mouse Monoclonal ELISA,FCM 10730-MM05
    Anti-DDR1 Antibody Rabbit Polyclonal ELISA 10730-T16
    Anti-DDR1 Antibody Rabbit Monoclonal ELISA(Det) 10730-R017
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