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DDR1 Kinase / MCK10 / CD167 Antibody, Mouse MAb

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Human DDR1 Antibody Product Information
Immunogen:Recombinant Human DDR1 protein (Catalog#10730-H08H)
Clone ID:10B6E5A11
Ig Type:Mouse IgG1
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human DDR1 / CD167a (rh DDR1; Catalog#10730-H08H; NP_001945.3; Met 1-Thr 416). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Human DDR1 Antibody Usage Guide
Specificity:Human DDR1 / CD167a
No cross-reactivity with Human cell lysate (293 cell line) in WB and ELISA.
Application:ELISA, FCM

ELISA: 0.5-1 μg/mL

This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human DDR1. The detection limit for Human DDR1 is 0.16 ng/well.

FCM: 0.5-2 μg/Test

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Human DDR1 Antibody FC Application Image
DDR1 / MCK10 / CD167 Antibody, Mouse MAb, Flow Cytometry
[Click to enlarge image]
Flow cytometric analysis of Human DDR1(CD167a) expression on MCF-7 cells. Cells were stained with purified anti-Human DDR1(CD167a), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
Other DDR1 Antibody Products
DDR1/MCK10/CD167 Background

Discoidin domain receptor family, member 1 (DDR1), also known as or CD167a (cluster of differentiation 167a), and Mammary carcinoma kinase 10 (MCK10), belongs to a subfamily of tyrosine kinase receptors with an extracellular domain homologous to Dictyostellium discoideum protein discoidin 1. Receptor tyrosine kinases play a key role in the communication of cells with their microenvironment. These kinases are involved in the regulation of cell growth, differentiation and metabolism. Expression of DDR1/MCK10/CD167 is restricted to epithelial cells, particularly in the kidney, lung, gastrointestinal tract, and brain. In addition, it has been shown to be significantly overexpressed in several human tumors. DDR1/MCK10/CD167 plays an important role in regulating attachment to collagen, chemotaxis, proliferation, and MMP production in smooth muscle cells. DDR1 functions in a feedforward loop to increase p53 levels and at least some of its effectors. Inhibition of DDR1 function resulted in strikingly increased apoptosis of wild-type p53-containing cells in response to genotoxic stress through a caspase-dependent pathway.

Human DDR1/MCK10/CD167 References
  • Hou G, et al. (2001) The discoidin domain receptor tyrosine kinase DDR1 in arterial wound repair. J Clin Invest. 107(6): 727-35.
  • Ongusaha PP, et al. (2003) p53 induction and activation of DDR1 kinase counteract p53-mediated apoptosis and influence p53 regulation through a positive feedback loop. EMBO J. 22(6): 1289-301.
  • Jönsson M, et al. (2001) Repression of Wnt-5a impairs DDR1 phosphorylation and modifies adhesion and migration of mammary cells. J Cell Sci. 114(11): 2043-53.
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    Catalog: 10730-MM05-50
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