Anti-DDOST Antibody

Cat: 12463-RP02
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Anti-DDOST Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-DDOST Antibody
Validated applications
WB,ELISA,IHC-P
Species reactivity
Reacts with: Human
Specificity
Human DDOST
Immunogen
Recombinant Human DDOST / OST48 protein (Catalog#12463-HNCE)
Preparation
Produced in rabbits immunized with purified, recombinant Human DDOST / OST48 (rh DDOST / OST48; Catalog#12463-HNCE; P39656-1; Ser 43-Pro 427). DDOST / OST48 specific IgG was purified by Human DDOST / OST48 affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-DDOST Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:1000  
ELISA 1:25000-1:50000  
IHC-P 1:2000-1:10000  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-DDOST Antibody (Rabbit Polyclonal antibody) Images
Human DDOST/OST48 Immunohistochemistry(IHC) 4298
Immunochemical staining of human DDOST in human pancreas with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections. Positive staining was localized to pancreatic acinus.
Human DDOST/OST48 Immunohistochemistry(IHC) 4299
Immunochemical staining of human DDOST in human liver with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections. Positive staining was localized to hepatocyte.
Human DDOST/OST48 Immunohistochemistry(IHC) 4300
Immunochemical staining of human DDOST in human heart with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections. Positive staining was localized to cardiac muscle.
Human DDOST/OST48 Western blot (WB) 13493

Anti-DDOST rabbit polyclonal antibody at 1:500 dilution

Lane A: Jurkat Membrane Lysate

Lysates/proteins at 20 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:51 kDa

Observed band size:45 kDa

Anti-DDOST Antibody Alternative Names
Anti-AGER1 Antibody;Anti-CDG1R Antibody;Anti-OKSWcl45 Antibody;Anti-OST Antibody;Anti-OST48 Antibody;Anti-WBP1 Antibody
DDOST Background Information

The enzyme oligosaccharyltransferase (dolichyl-diphosphooligosaccharide-protein glycosyltransferase) (DDOST), or 48-kDa subunit (OST48) is one of the catalytic subunits in this complex, exerts a typical type I membrane topology, containing a large luminal domain, a hydrophobic transmembrane domain and a short cytosolic peptide tail. DDOST/OST48 catalyzes the transfer of a high-mannose oligosaccharide (GlcNac2Man9Glc3) from a dolichol-linked oligosaccharide donor (dolichol-P-GlcNac2Man9Glc3) onto the asparagine acceptor site within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains across the membrane of the endoplasmic reticulum. The mammalian oligosaccharyltransferase (OST) is an oligomeric complex composed of three type I transmembrane proteins of the endoplasmic reticulum: ribophorin I (RI), ribophorin II (RII), and OST48. OST48 is not a glycoprotein and is not recognized by antibodies to either ribophorin. Like ribophorins I and II, OST48 was found to be an integral membrane protein, with the majority of the polypeptide located within the lumen of the endoplasmic reticulum (ER). OST48 does not show significant amino acid sequence homology to either ribophorin I or II. It had been found that only the luminal domain of RI contains ER retention information. The dilysine motif in OST48 functions as an ER localization motif because OST48 in which the two lysine residues are replaced by serine (OST48ss) is no longer retained in the ER and is found instead also at the plasma membrane.

Full Name
dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit (non-catalytic)
References
  • Silberstein S, et al. (1992) The 48-kDa subunit of the mammalian oligosaccharyltransferase complex is homologous to the essential yeast protein WBP1. J Biol Chem. 267(33): 23658-63.
  • Fu J, et al. (1997) Interactions among subunits of the oligosaccharyltransferase complex. J Biol Chem. 272(47): 29687-92.
  • Yamagata T, et al. (1997) Genomics. Genome organization of human 48-kDa oligosaccharyltransferase (DDOST). 45(3): 535-40.
  • Fu J, et al. (2000) Retention of subunits of the oligosaccharyltransferase complex in the endoplasmic reticulum. J Biol Chem. 275(6): 3984-90.
  • Hardt B, et al. (2001) Analysis of structural signals conferring localisation of pig OST48 to the endoplasmic reticulum. Biol Chem. 382(7): 1039-47.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-DDOST Antibody Mouse Monoclonal ELISA 12463-MM02
    info info
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