DDOST / OST48 Antibody (Antigen Affinity Purified)

DDOST / OST48 Antibody ( Antigen Affinity Purified ) Datasheet

DDOST / OST48 Antibody Product Information

• Immunogen: Recombinant Human DDOST / OST48 protein (Catalog#12463-HNCE)
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, recombinant Human DDOST / OST48 (rh DDOST / OST48; Catalog#12463-HNCE; P39656-1; Ser 43-Pro 427). DDOST / OST48 specific IgG was purified by Human DDOST / OST48 affinity chromatography.

DDOST / OST48 Antibody Usage Guide

• Specificity: Human DDOST / OST48
• Western blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human DDOST in WB. Using a DAB detection system, the detection limit for Human DDOST is approximately 0.5 ng/lane under non-reducing conditions and reducing conditions.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human DDOST. The detection limit for Human DDOST is approximately 0.039 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

DDOST / OST48 Antibody Background

The enzyme oligosaccharyltransferase (dolichyl-diphosphooligo-saccharide-protein glycosyl-transferase) (DDOST), also known as 48-kDa subunit (OST48), catalyzes the transfer of a high-mannose oligosaccharide (GlcNac2Man9Glc3) from a dolichol-linked oligosaccharide donor (dolichol-P-GlcNac2Man9Glc3) onto the asparagine acceptor site within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains across the membrane of the endoplasmic reticulum. DDOST mRNA is expressed intensely in heart and pancreas, but at lower levels in brain. OST48, one of the catalytic subunits in this complex, exerts a typical type I membrane topology, containing a large luminal domain, a hydrophobic transmembrane domain and a short cytosolic peptide tail. It is not a glycoprotein and is not recognized by antibodies to either ribophorin. Like ribophorins I and II, OST48 was found to be an integral membrane protein, with the majority of the polypeptide located within the lumen of the endoplasmic reticulum. OST48 does not show significant amino acid sequence homology to either ribophorin I or II.

References

1. Yamagata T. et al., 1997, Genomics. 45 (3): 535-40.
2. Hardt B. et al., 2001, Biol Chem. 382 (7): 1039-47.
3. Silberstein S. et al., 1992, J Biol Chem. 267 (33): 23658-63.