Mouse DDC / AADC Protein (His Tag)

Dopa decarboxylase Protein Datasheet

DDC / AADC Protein Product Information

• Synonym: RP23-373H2.3, Aadc
• Protein Construction: A DNA sequence encoding the mouse DDC (O88533) (Met 1-Glu 480) was expressed，with a C-terminal polyhistidine tag.
• Source: Mouse
• Expression Host: Baculovirus-Insect cells

DDC / AADC Protein QC Testing

• Purity: > 90 % as determined by SDS-PAGE.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant mouse DDC consists of 490 amino acids and has a calculated molecular mass of 55.2 kDa. It migrates as an approximately 50 KDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from 0.2μm filtered solution of 50mM Tris, 100mM NaCl, pH8.0, 20%gly, 3mM DTT
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: DDC / AADC protein

DDC / AADC Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

DDC / AADC Protein Description

Dopa Decarboxylase (DDC), also known as AADC and Aromatic-L-amino acid decarboxylase, is a 54 kDa member of the group II decarboxylase family of proteins.It is a vitamin B6-dependent homodimeric enzyme that catalyzes the decarboxylation of both L-3,4-dihydroxyphenylalanine (L-DOPA) and L-5-hydroxytryptophan to dopamine and serotonin, respectively, which are major mammalian neurotransmitters and hormones belonging to catecholamines and indoleamines. Since L-DOPA is regularly used to treat the symptoms of Parkinson's disease, the catalytic pathway is of particular research interest. Defects of DDC are associated with severe developmental delay, oculogyric crises (OGC), as well as autosomal recessive disorder AADC deficiency, an early onset inborn error in neurotransmitter metabolism which can lead to catecholamine and serotonin deficiency.

References

1. Ichinose H. et al.,1989, Biochem Biophys Res Commun. 164: 1024-30.
2. Lisa J S. et al., 1992, Genomics 13: 469-71.
3. Moore P S. et al.,1996, Biochem J. 315: 249-56.
4. Bertoldi M. et al., 2003, Biochim Biophys Acta. 1647: 42-47.
5. Vassilacopoulou D. et al., 2004, Neurochem Res. 29: 1817-23.
6. Ma JZ. et al., 2005, Hum Mol Genet. 14: 1691-8.