Human DCT Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13576-G	Cloning vector ($195) pCMV / hygro ($395) pCMV / hygro - HA ($395) pCMV / hygro - His ($395) pCMV / hygro - Myc ($395) pCMV / hygro - FLAG ($395)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

DCT cDNA Clone Datasheet

DCT cDNA Clone Product Information

Gene Bank Ref.ID:	BC028311
cDNA Size:	1560
Gene Synonym:	TRP-2;TYRP2;DCT
Source:	Human

DCT cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens dopachrome tautomerase (dopachrome delta-isomerase, tyrosine-related protein 2) DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

DCT cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

DCT cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

DCT cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAGCCCCC TTTGGTGGGG GTTTCTGCTC AGTTGCTTGG GCTGCAAAAT CCTGCCAGGA
61       GCCCAGGGTC AGTTCCCCCG AGTCTGCATG ACGGTGGACA GCCTAGTGAA CAAGGAGTGC
121      TGCCCACGCC TGGGTGCAGA GTCGGCCAAT GTCTGTGGCT CTCAGCAAGG CCGGGGGCAG
181      TGCACAGAGG TGCGAGCCGA CACAAGGCCC TGGAGTGGTC CCTACATCCT ACGAAACCAG
241      GATGACCGTG AGCTGTGGCC AAGAAAATTC TTCCACCGGA CCTGCAAGTG CACAGGAAAC
301      TTTGCCGGCT ATAATTGTGG AGACTGCAAG TTTGGCTGGA CCGGTCCCAA CTGCGAGCGG
361      AAGAAACCAC CAGTGATTCG GCAGAACATC CATTCCTTGA GTCCTCAGGA AAGAGAGCAG
421      TTCTTGGGCG CCTTAGATCT CGCGAAGAAG AGAGTACACC CCGACTACGT GATCACCACA
481      CAACACTGGC TGGGCCTGCT TGGGCCCAAT GGAACCCAGC CGCAGTTTGC CAACTGCAGT
541      GTTTATGATT TTTTTGTGTG GCTCCATTAT TATTCTGTTA GAGATACATT ATTAGGACCA
601      GGACGCCCCT ACAGGGCCAT AGATTTCTCA CATCAAGGAC CTGCATTTGT TACCTGGCAC
661      CGGTACCATT TGTTGTGTCT GGAAAGAGAT CTCCAGCGAC TCATTGGCAA TGAGTCTTTT
721      GCTTTGCCCT ACTGGAACTT TGCCACTGGG AGGAACGAGT GTGATGTGTG TACAGACCAG
781      CTGTTTGGGG CAGCGAGACC AGACGATCCG ACTCTGATTA GTCGGAACTC AAGATTCTCC
841      AGCTGGGAAA CTGTCTGTGA TAGCTTGGAT GACTACAACC ACCTGGTCAC CTTGTGCAAT
901      GGAACCTATG AAGGTTTGCT GAGAAGAAAT CAAATGGGAA GAAACAGCAT GAAATTGCCA
961      ACCTTAAAAG ACATACGAGA TTGCCTGTCT CTCCAGAAGT TTGACAATCC TCCCTTCTTC
1021     CAGAACTCTA CCTTCAGTTT CAGGAATGCT TTGGAAGGGT TTGATAAAGC AGATGGGACT
1081     CTGGATTCTC AAGTGATGAG CCTTCATAAT TTGGTTCATT CCTTCCTGAA CGGGACAAAC
1141     GCTTTGCCAC ATTCAGCCGC CAATGATCCC ATTTTTGTGG TTCTTCATTC CTTTACTGAT
1201     GCCATCTTTG ATGAGTGGAT GAAAAGATTT AATCCTCCTG CAGATGCCTG GCCTCAGGAG
1261     CTGGCCCCTA TTGGTCACAA TCGGATGTAC AACATGGTTC CTTTCTTCCC TCCAGTGACT
1321     AATGAAGAAC TCTTTTTAAC CTCAGACCAA CTTGGCTACA GCTATGCCAT CGATCTGCCA
1381     GTTTCAGTTG AAGAAACTCC AGGTTGGCCC ACAACTCTCT TAGTAGTCAT GGGAACACTG
1441     GTGGCTTTGG TTGGTCTTTT TGTGCTGTTG GCTTTTCTTC AATATAGAAG ACTTCGAAAA
1501     GGATATACAC CCCTAATGGA GACACATTTA AGCAGCAAGA GATACACAGA AGAAGCCTAG

Quality Control:cDNA sequencing results is available upon request.