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> ELISA > ELISA Pair Set > DCIR / CLEC4A / CLECSF6 ELISA Pair Set, Human ELISA Pair Set DCIR / CLEC4A / CLECSF6 ELISA Pair Set, Human ELISA Pair Set 
| Catalog | Size (Price) | Quantity | In Stock | Operation | Other Information |
| SEK11476 |
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Human DCIR / CLEC4A / CLECSF6 ELISA Pair Set
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Order or Inquire for DCIR / CLEC4A / CLECSF6 ELISA Pair Set |
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High affinity Human DCIR / CLEC4A / CLECSF6 ELISA Pair Set |
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Detection limit - 9.375 pg/ml |
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Affordable price and 30%-80% cost saving for Bulk order |
- Materials
- Protocol
- Trouble Shooting
- Image & Flash
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No signal |
Incorrect or no Detection Antibody was added |
Add appropriate Detection Antibody and continue |
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Substrate solution was not added |
Add substrate solution and continue |
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Incorrect storage condition |
Check if the kit is stored at recommended condition and used before expiration date |
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Poor Standard Curve |
Standard was incompletely reconstituted or was inappropriately stored |
Aliquot reconstituted standard and store at -70 ℃ |
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Imprecise / inaccurate pipetting |
Check / calibrate pipettes |
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Incubations done at inappropriate temperature, timing or agitation |
Follow the general ELISA protocol |
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Background wells were contaminated |
Avoid cross contamination by using the sealer appropriately |
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Poor detection value |
The concentration of antigen in samples was too low |
Enriching samples to increase the concentration of antigen |
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Samples were ineffective |
Check if the samples are stored at cold environment. Detect samples in timely manner |
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High Background |
Insufficient washes |
Use multichannel pipettes without touching the reagents on the plate |
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Increase cycles of washes and soaking time between washes |
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TMB Substrate Solution was contaminated |
TMB Substrate Solution should be clear and colorless prior to addition to wells |
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Materials were contaminated |
Use clean plates, tubes and pipettes tips |
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Non-specificity |
Samples were contaminated |
Avoid cross contamination of samples |
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The concentration of samples was too high |
Try higher dilution rate of samples |
Problems 
Possible Sources 
Solutions 
Human DCIR / CLEC4A / CLECSF6 ELISA Pair Set Related Products & Topics
Related Areas:
Immunology>>Adhesion Molecule>>Lectin>>DCIR/CLEC4A/CLECSF6
Proteins:
| Molecule | Species | Description //For Detailed Info. and Price------CLICK! | Cat. No |
| DCIR/CLEC4A/CLECSF6 | Human | DCIR/CLEC4A/CLECSF6 Protein, Recombinant | 11476-H07H |
Antibodies:
| Molecule | Application | Description //For Detailed Info. and Price------CLICK! | Cat. No |
| Human DCIR/CLEC4A/CLECSF6 |
WB, ELISA | DCIR/CLEC4A/CLECSF6 Antibody, Mouse MAb | 11476-MM02 |
| Human DCIR/CLEC4A/CLECSF6 |
WB, ELISA | DCIR/CLEC4A/CLECSF6 Antibody, Mouse MAb | 11476-MM08 |
| Human DCIR/CLEC4A/CLECSF6 |
WB, ELISA | DCIR/CLEC4A/CLECSF6 Antibody, Rabbit PAb | 11476-RP01 |
| Human DCIR/CLEC4A/CLECSF6 |
WB, ELISA | DCIR/CLEC4A/CLECSF6 Antibody, Rabbit PAb (Antigen Affinity Purified) | 11476-RP02 |
Background
C-type lectin domain family 4 member A, also known as C-type lectin superfamily member 6, Dendritic cell immunoreceptor, Lectin-like immunoreceptor, C-type lectin DDB27, CLEC4A and CLECSF6, is a single-pass type I I membrane protein which contains one C-type lectin domain. CLEC4A / CLECSF6 is expressed preferentially in hematopoietic tissues. It is expressed in peripheral blood leukocytes, neutrophils, moderate quantities in spleen, lymph node, and bone marrow, and at very low levels in thymus. CLEC4A / CLECSF6 contains one copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases. CLEC4A / CLECSF6 may be involved in regulating immune reactivity. It may play a role in modulating dendritic cells (DC) differentiation and/or maturation. It may also be involved via its ITIM motif (immunoreceptor tyrosine-based inhibitory motifs) in the inhibition of B-cell-receptor-mediated calcium mobilization and protein tyrosine phosphorylation.
