Anti-CLEC4A Antibody

Cat: 11476-MM02
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Anti-CLEC4A Antibody (Mouse Monoclonal antibody) General Information
Product name
Anti-CLEC4A Antibody
Validated applications
ELISA,ELISA(Det)
Species reactivity
Reacts with: Human
Specificity
Human CLEC4A
Immunogen
Recombinant Human CLEC4A protein (Catalog#11476-H07H)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CLEC4A (rh CLEC4A; Catalog#11476-H07H; NP_001128530.1; Gln 70-Leu 237). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG1 Clone #7D4B5
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-CLEC4A Antibody (Mouse Monoclonal antibody) Validated Applications
Application Dilution Notes
ELISA 1:1000-1:2000  
ELISA(Det) 1:1000-1:10000 This antibody will detect Human DCIR / CLEC4A / CLECSF6 in ELISA pair set (Catalog: # SEK11476). In a sandwich ELISA, it can be used as detection antibody when paired with (Catalog: # 11476-MM04).

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-CLEC4A Antibody Alternative Names
Anti-CD367 Antibody;Anti-CLEC4A Antibody;Anti-CLECSF6 Antibody;Anti-DCIR Antibody;Anti-DDB27 Antibody;Anti-HDCGC13P Antibody;Anti-LLIR Antibody
CLEC4A Background Information

Dendritic cell immunoreceptor (DCIR), also known as C-type lectin domain family 4 member A (CLEC4A), C-type lectin superfamily member 6 (CLECSF6), is a single-pass type II C-type lectin receptor expressed mainly in dendritic cells (DCs), which is a negative regulator of DC expansion and has a crucial role in maintaining the homeostasis of the immune system. The Dectin-2 family of C-type lectins that includes Dectin-2, BDCA-2, DCIR, DCAR, Clecsf8 and Mincle. These type II receptors contain a single extracellular carbohydrate recognition domain and have diverse functions in both immunity and homeostasis. DCIR is the only member of the family which contains a cytoplasmic signalling motif and has been shown to act as an inhibitory receptor, while BDCA-2, Dectin-2, DCAR and Mincle all associate with FcRgamma chain to induce cellular activation, including phagocytosis and cytokine production. Dectin-2 and Mincle have been shown to act as pattern recognition receptors for fungi, while DCIR acts as an attachment factor for HIV. In addition to pathogen recognition, DCIR has been shown to be pivotal in preventing autoimmune disease by controlling dendritic cell proliferation. DCIR expressed on antigen presenting cells and granulocytes and acts as an inhibitory receptor via an intracellular immunoreceptor tyrosine-based inhibitory motif (ITIM). It may also be involved via its ITIM motif in the inhibition of B-cell-receptor-mediated calcium mobilization and protein tyrosine phosphorylation. Additionally, DCIR can participate in the capture of HIV-1 and promote infection in trans and in cis of autologous CD4(+) T cells from human immature monocyte-derived DCs. DCIR acts as a ligand for HIV-1 and is involved in events leading to productive virus infection.

Full Name
C-type lectin domain family 4 member A
References
  • Kanazawa N, et al. (2004) Signaling and immune regulatory role of the dendritic cell immunoreceptor (DCIR) family lectins: DCIR, DCAR, dectin-2 and BDCA-2. Immunobiology. 209(1-2): 179-90.
  • Fujikado N, et al. (2008) Dcir deficiency causes development of autoimmune diseases in mice due to excess expansion of dendritic cells. Nat Med. 14(2): 176-80.
  • Lambert AA, et al. (2008) The C-type lectin surface receptor DCIR acts as a new attachment factor for HIV-1 in dendritic cells and contributes to trans- and cis-infection pathways. Blood. 112(4): 1299-307.
  • Graham LM, et al. (2009) The Dectin-2 family of C-type lectins in immunity and homeostasis. Cytokine. 48(1-2): 148-55.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-CLEC4A Antibody Rabbit Polyclonal ELISA 11476-RP01
    Anti-CLEC4A Antibody Rabbit Monoclonal ELISA 11476-R007
    Anti-CLEC4A Antibody Rabbit Polyclonal ELISA 11476-RP02
    Anti-CLEC4A Antibody (FITC) Mouse Monoclonal FCM 11476-MM08-F
    Anti-CLEC4A Antibody (PE) Mouse Monoclonal FCM 11476-MM08-P
    Anti-CLEC4A Antibody Mouse Monoclonal ELISA,ELISA(Cap) 11476-MM04
    Anti-CLEC4A Antibody Mouse Monoclonal ELISA,FCM 11476-MM08
    Anti-CLEC4A Antibody Mouse Monoclonal IHC-P 11476-MM06
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