Human DCBLD2 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13615-G	Cloning vector ($195) pCMV / hygro ($445) pCMV / hygro - HA ($445) pCMV / hygro - His ($445) pCMV / hygro - Myc ($445) pCMV / hygro - FLAG ($445)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

DCBLD2 cDNA Clone Datasheet

DCBLD2 cDNA Clone Product Information

Gene Bank Ref.ID:	BC029658
cDNA Size:	2232
Gene Synonym:	ESDN;CLCP1;DCBLD2
Source:	Human

DCBLD2 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens discoidin, CUB and LCCL domain containing 2 DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref. ID sequence except for the point mutation 525 G/T resulting in the amino acid Glu substitution by Asp.

DCBLD2 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

DCBLD2 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

DCBLD2 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCCTCTGT TCCTCCTGCT CTTACTTGTC CTGCTGCTGC TGCTCGAGGA CGCTGGAGCC
61       CAGCAAGGTG ATGGATGTGG ACACACTGTA CTAGGCCCTG AGAGTGGAAC CCTTACATCC
121      ATAAACTACC CACAGACCTA TCCCAACAGC ACTGTTTGTG AATGGGAGAT CCGTGTAAAG
181      ATGGGAGAGA GAGTTCGCAT CAAATTTGGT GACTTTGACA TTGAAGATTC TGATTCTTGT
241      CACTTTAATT ACTTGAGAAT TTATAATGGA ATTGGAGTCA GCAGAACTGA AATAGGCAAA
301      TACTGTGGTC TGGGGTTGCA AATGAACCAT TCAATTGAAT CAAAAGGCAA TGAAATCACA
361      TTGCTGTTCA TGAGTGGAAT CCATGTTTCT GGACGCGGAT TTTTGGCCTC ATACTCTGTT
421      ATAGATAAAC AAGATCTAAT TACTTGTTTG GACACTGCAT CCAATTTTTT GGAACCTGAG
481      TTCAGTAAGT ACTGCCCAGC TGGTTGTCTG CTTCCTTTTG CTGATATATC TGGAACAATT
541      CCTCATGGAT ATAGAGATTC CTCGCCATTG TGCATGGCTG GTGTGCATGC AGGAGTAGTG
601      TCAAACACGT TGGGCGGCCA AATCAGTGTT GTAATTAGTA AAGGTATCCC CTATTATGAA
661      AGTTCTTTGG CTAACAACGT CACATCTGTG GTGGGACACT TATCTACAAG TCTTTTTACA
721      TTTAAGACAA GTGGATGTTA TGGAACACTG GGGATGGAGT CTGGTGTGAT CGCGGATCCT
781      CAAATAACAG CATCATCTGT GCTGGAGTGG ACTGACCACA CAGGGCAAGA GAACAGTTGG
841      AAACCCAAAA AAGCCAGGCT GAAAAAACCT GGACCGCCTT GGGCTGCTTT TGCCACTGAT
901      GAATACCAGT GGTTACAAAT AGATTTGAAT AAGGAAAAGA AAATAACAGG CATTATAACC
961      ACTGGATCCA CCATGGTGGA GCACAATTAC TATGTGTCTG CCTACAGAAT CCTGTACAGT
1021     GATGATGGGC AGAAATGGAC TGTGTACAGA GAGCCTGGTG TGGAGCAAGA TAAGATATTT
1081     CAAGGAAACA AAGATTATCA CCAGGATGTG CGTAATAACT TTTTGCCACC AATTATTGCA
1141     CGTTTTATTA GAGTGAATCC TACCCAATGG CAGCAGAAAA TTGCCATGAA AATGGAGCTG
1201     CTCGGATGTC AGTTTATTCC TAAAGGTCGT CCTCCAAAAC TTACTCAACC TCCACCTCCT
1261     CGGAACAGCA ATGACCTCAA AAACACTACA GCCCCTCCAA AAATAGCCAA AGGTCGTGCC
1321     CCAAAATTTA CGCAACCACT ACAACCTCGC AGTAGCAATG AATTTCCTGC ACAGACAGAA
1381     CAAACAACTG CCAGTCCTGA TATCAGAAAT ACTACCGTAA CTCCAAATGT AACCAAAGAT
1441     GTAGCGCTGG CTGCAGTTCT TGTCCCTGTG CTGGTCATGG TCCTCACTAC TCTCATTCTC
1501     ATATTAGTGT GTGCTTGGCA CTGGAGAAAC AGAAAGAAAA AAACTGAAGG CACCTATGAC
1561     TTACCTTACT GGGACCGGGC AGGTTTTTAT TTAATGGTCT CTTTGGCTTG CCGTCACAAT
1621     GAAGGTTGGT GGAAAGGAAT GAAGCAGTTT CTTCCTGCAA AAGCAGTGGA CCATGAGGAA
1681     ACCCCAGTTC GCTATAGCAG CAGTGAAGTT AATCACCTGA GTCCAAGAGA AGTCACCACA
1741     GTGCTGCAGG CTGACTCTGC AGAGTATGCT CAGCCACTGG TAGGAGGAAT TGTTGGTACA
1801     CTTCATCAAA GATCTACCTT TAAACCAGAA GAAGGAAAAG AAGCAGGCTA TGCAGACCTA
1861     GATCCTTACA ACTCACCAGG GCAGGAAGTT TATCATGCCT ATGCTGAACC ACTCCCAATT
1921     ACGGGGCCTG AGTATGCAAC CCCAATCATC ATGGACATGT CAGGGCACCC CACAACTTCA
1981     GTTGGTCAGC CCTCCACATC CACTTTCAAG GCTACGGGGA ACCAACCTCC CCCACTAGTG
2041     GGAACTTACA ATACACTTCT CTCCAGGACT GACAGCTGCT CCTCAGCCCA GGCCCAGTAT
2101     GATACCCCGA AAGCTGGGAA GCCAGGTCTA CCTGCCCCAG ACGAATTGGT GTACCAGGTG
2161     CCACAGAGCA CACAAGAAGT ATCAGGAGCA GGAAGGGATG GGGAATGTGA TGTTTTTAAA
2221     GAAATCCTTT GA

Quality Control:cDNA sequencing results is available upon request.