DCAMKL1 / DCLK1 (aa 1-705) Protein (GST Tag)

Doublecortin-like kinase 1 Protein Datasheet

DCAMKL1 / DCLK1 Protein Product Information

• Synonym: RP11-113P14.1, CL1, CLICK1, DCAMKL1, DCDC3A, DCLK
• Protein Construction: A DNA sequence encoding the human DCLK1 (O15075-1) (Met 1-Val 705) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
• Source: Human
• Expression Host: Baculovirus-Insect cells

DCAMKL1 / DCLK1 Protein QC Testing

• Purity: > 84 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant human DCLK1/GST chimera consists of 942 amino acids and has a calculated molecular mass of 106 KDa. It migrates as an approximately 105 KDa band as predicted in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH7.4，10%gly, 0.5mM PMSF.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: DCAMKL1 / DCLK1 protein

DCAMKL1 / DCLK1 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

DCAMKL1 / DCLK1 Protein Description

ADCAMKL1, also known as DCLK1, is a member of the protein kinase superfamily and the doublecortin family. It contains 2 doublecortin domains and 1 protein kinase domain. DCAMKL1 plays a role in in several different cellular processes, including neuronal migration, retrograde transport, neuronal apoptosis and neurogenesis. The microtubule-polymerizing activity of DCAMKL1 is independent of its protein kinase activity. It is expressed differently in fetal and adult tissues. In fetal tissues, it is highly expressed in brain, detectable in lung and liver, but not in kidney. While in adult tissues, DCAMKL1 is expressed ubiquitously in the brain, detectable in the heart, liver, spleen, thymus, prostate, testis, ovary, small intestine and colon. Multiple transcript variants generated by two alternative promoter usage and alternative splicing have been reported. These variants encode different isoforms, which are differentially expressed and have different kinase activities. DCAMKL1 may be involved in a calcium-signaling pathway controlling neuronal migration in the developing brain. It may also participate in functions of the mature nervous system.

References

1. Sossey-Alaoui K. et al., 1999, Genomics. 56 (1): 121-6.
2. Matsumoto N. et al., 1999, Genomics. 56 (2): 179-83.
3. Lin PT. et al., 2001, J Neurosci. 20 (24): 9152-61.