Anti-DC-SIGN Antibody

Cat: 10200-R061
Contact us:
215-583-7898
order@sinobiological.com
Distributors
All DC-SIGN Reagents
CRO Services
Product recommended

All DC-SIGN Reagents
Anti-DC-SIGN Antibody (Rabbit Monoclonal antibody) General Information
Product name
Anti-DC-SIGN Antibody
Validated applications
WB,ELISA
Species reactivity
Reacts with: Human
Specificity
Human DC-SIGN
Immunogen
Recombinant Human DC-SIGN / CD209 protein (Catalog#10200-H01H)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human DC-SIGN / CD209 (rh DC-SIGN / CD209; Catalog#10200-H01H; NP_066978.1; Lys62-Ala404).
Source
Monoclonal Rabbit IgG Clone #061
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-DC-SIGN Antibody (Rabbit Monoclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:1000  
ELISA 1:25000-1:50000  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-DC-SIGN Antibody (Rabbit Monoclonal antibody) Images
Human CD209/DC-SIGN Western blot (WB) 13141

Anti-CD209 rabbit monoclonal antibody at 1:500 dilution

Lane A: THP1 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:46 kDa

Observed band size:46 kDa

(We are unsure as to the identity of these extra bands.)

Anti-DC-SIGN Antibody Alternative Names
Anti-CD209 Antibody;Anti-CDSIGN Antibody;Anti-CLEC4L Antibody;Anti-DC-SIGN Antibody;Anti-DC-SIGN1 Antibody;Anti-MGC129965 Antibody
DC-SIGN Background Information

Dendritic cell (DC)-specific intercellular adhesion molecule 3 (ICAM-3) grabbing nonintegrin (DC-SIGN), also known as CD209, is a type II transmembrane protein on DCs with a C-type lectin extracellular domain, is capable of binding ICAM-3 on resting T cells in the secondary lymphoid organs, providing the initial contact between these cells during the establishment of cell-mediated immunity. It is not only a pattern recognition receptor but implicated in immunoregulation of DCs. It has important role in mediating DC adhesion, migration, inflammation, activating primary T cell, triggering immune response and participating in immune escape of pathogens and tumors. DC-SIGN also mediates capture and internalization of viral, bacterial, and fungal pathogens by dendritic cells, such as HIV-1, Ebola virus, cytomegalovirus, Dengue virus, and hepatitis C virus. DC-SIGN is unique in that it regulates adhesion processes, such as DC trafficking and T-cell synapse formation, as well as antigen capture. Moreover, even though several C-type lectins have been shown to bind HIV-1, DC-SIGN does not only capture HIV-1 but also protects it in early endosomes allowing HIV-1 transport by DC to lymphoid tissues, where it enhances trans infection of T cells.

Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Full Name
CD209 molecule
References
  • Geijtenbeek TB, et al. (2002) DC-SIGN, a C-type lectin on dendritic cells that unveils many aspects of dendritic cell biology. J Leukoc Biol. 71(6): 921-31.
  • Masso M. (2003) DC-SIGN points the way to a novel mechanism for HIV-1 transmission. MedGenMed. 5(2): 2.
  • Zhou T, et al. (2006) DC-SIGN and immunoregulation. Cell Mol Immunol. 3(4): 279-83.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-DC-SIGN Antibody (FITC) Rabbit Monoclonal FCM 10200-R037-F
    Anti-DC-SIGN Antibody (FITC) Mouse Monoclonal FCM 10200-MM01-F
    Anti-DC-SIGN Antibody Mouse Monoclonal ELISA(Cap),FCM 10200-MM01
    Anti-DC-SIGN Antibody Rabbit Polyclonal ELISA 10200-T16
    Anti-DC-SIGN Antibody (PE) Mouse Monoclonal FCM 10200-MM01-P
    Anti-DC-SIGN Antibody Rabbit Monoclonal FCM 10200-R037
    Anti-DC-SIGN Antibody Rabbit Monoclonal ELISA(Det) 10200-R059
    Anti-DC-SIGN Antibody (APC) Mouse Monoclonal FCM 10200-MM01-A
    Anti-DC-SIGN Antibody (APC) Rabbit Monoclonal FCM 10200-R037-A
    Anti-DC-SIGN Antibody (PE) Rabbit Monoclonal FCM 10200-R037-P
    Anti-DC-SIGN Antibody Rabbit Polyclonal ELISA 10200-RP01
    info info
    添加购物车成功! 添加购物车失败!