Human DBC1 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13364-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

DBC1 cDNA Clone Datasheet

DBC1 cDNA Clone Product Information

Gene Bank Ref.ID:	BC021560
cDNA Size:	963
Gene Synonym:	FAM5A; DBCCR1; DBC1
Source:	Human

DBC1 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens deleted in bladder cancer 1 DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

DBC1 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

DBC1 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

DBC1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAACTGGA GGTTTGTTGA GCTCCTCTAC TTCCTGTTTA TATGGGGCCG TATCTCAGTG
61       CAGCCCTCCC ACCAGGAACC AGCTGGGACA GACCAACATG TCTCCAAGGA ATTTGATTGG
121      CTCATTTCAG ACAGGGGGCC TTTCCACCAC TCCAGGAGCT ACCTATCCTT TGTGGAAAGA
181      CACCGTCAAG GATTTACAAC CAGATATAAA ATATACAGGG AGTTTGCCCG TTGGAAGGTG
241      AGGAACACAG CCATCGAGAG GAGAGATCTG GTCCGCCATC CAGTGCCCCT CATGCCGGAG
301      TTTCAAAGGA GCATCCGCCT GCTTGGCAGG AGACCTACCA CTCAGCAGTT CATCGATACC
361      ATCATCAAAA AGTACGGCAC CCACCTGCTC ATCTCAGCCA CATTGGGAGG GGAGGAGGCT
421      TTGACCATGT ATATGGACAA AAGTCGCCTC GACAGGAAGT CAGGGAATGC CACTCAAAGT
481      GTTGAAGCTC TGCACCAGCT CGCATCATCC TACTTTGTTG ACCGTGATGG TACCATGAGG
541      AGGCTTCATG AGATCCAGAT ATCAACTGGA GCAATCAAGG TCACAGAGAC ACGCACTGGG
601      CCTCTGGGCT GTAACAGTTA TGACAATCTG GACTCTGTGA GTTCCGTCCT TCTGCAAAGC
661      ACGGAGAGCA AACTGCACCT TCAAGGTCTT CAGATAATCT TTCCTCAGTA TCTGCAAGAG
721      AAGTTTGTCC AGTCGGCCTT GAGCTATATC ATGTGCAATG GGGAGGGGGA GTACCTGTGC
781      CAGAACAGCC AGTGTCGCTG CCAATGTGCC GAGGAGTTTC CGCAGTGCAA CTGCCCCATC
841      ACGGACATCC AGATCATGGA GTACACGCTG GCCAACATGG CCAAGTCTTG GGCCGAAGCT
901      TATAAGGACC TGGAGAATTC AGGTAGAGAG TCTCACTCAG TGCCACTGCA TGAGTGGCCT
961      TGA

Quality Control:cDNA sequencing results is available upon request.