Human DAZL Gene cDNA Clone / ORF Clone

DAZL cDNA Clone Datasheet

DAZL cDNA Clone Product Information

• Gene Bank Ref.ID: NM_001190811.1
• cDNA Size: 948
• Gene Synonym: DAZH, DAZL1, DAZLA, SPGYLA, MGC26406, DAZL
• Source: Human

DAZL cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens deleted in azoospermia-like DNA.
• Vector: pGEM-T vector

• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

DAZL cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

DAZL cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95^oC for 15–30 min .
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

DAZL cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCGGCTC CCTCGTGTGG CGGCGACAGA AAAGCTCGCC TGACGCCATC TTTGCCGCAC
61       GAGTCTACTG CAAATCCTGA AACTCCAAAC TCAACCATCT CCAGAGAGGC CAGCACCCAG
121      TCCTCATCAG CTGCAACCAG CCAAGGCTAT ATTTTACCAG AAGGCAAAAT CATGCCAAAC
181      ACTGTTTTTG TTGGAGGAAT TGATGTTAGG ATGGATGAAA CTGAGATTAG AAGCTTCTTT
241      GCTAGATATG GTTCAGTGAA AGAAGTGAAG ATAATCACTG ATCGAACTGG TGTGTCCAAA
301      GGCTATGGAT TTGTTTCATT TTTTAATGAC GTGGATGTGC AGAAGATAGT AGAATCACAG
361      ATAAATTTCC ATGGTAAAAA GCTGAAGCTG GGCCCTGCAA TCAGGAAACA AAATTTATGT
421      GCTTATCATG TGCAGCCACG TCCTTTGGTT TTTAATCATC CTCCTCCACC ACAGTTTCAG
481      AATGTCTGGA CTAATCCAAA CACTGAAACT TATATGCAGC CCACAACCAC GATGAATCCT
541      ATAACTCAGT ATGTTCAGGC ATATCCTACT TACCCAAATT CACCAGTTCA GGTCATCACT
601      GGATATCAGT TGCCTGTATA TAATTATCAG ATGCCACCAC AGTGGCCTGT TGGGGAGCAA
661      AGGAGCTATG TTGTACCTCC GGCTTATTCA GCTGTTAACT ACCACTGTAA TGAAGTTGAT
721      CCAGGAGCTG AAGTTGTGCC AAATGAATGT TCAGTTCATG AAGCTACTCC ACCCTCTGGA
781      AATGGCCCAC AAAAGAAATC TGTGGACCGA AGCATACAAA CGGTGGTATC TTGTCTGTTT
841      AATCCAGAGA ACAGACTGAG AAACTCTGTT GTTACTCAAG ATGACTACTT CAAGGATAAA
901      AGAGTGCATC ACTTTAGAAG AAGTCGGGCA ATGCTTAAAT CTGTTTGA

 

Quality Control:cDNA sequencing results is available upon request.