Human DAPK2 ORF Expression Clone, FLAG-tagged

DAPK2 cDNA Clone Datasheet

DAPK2 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_014326.3
• cDNA Size: 1048
• Gene Synonym: DRP1; DRP-1; MGC119312; DAPK2
• Source: Human

DAPK2 cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens death-associated protein kinase 2 DNA.
• Vector: pCMV/hygro-FLAG
• FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
• Sequence Description: Same as Gene Bank Ref. ID sequence with the nucleotide (223-314) deletion mutation and two point mutations 643 G/A which results in the amino acid Val replacement by Ile respectively.

DAPK2 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

DAPK2 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

DAPK2 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGTTCCAGG CCTCAATGAG GAGTCCAAAC ATGGAGCCAT TCAAGCAGCA GAAGGTGGAG
61       GACTTTTATG ACATCGGAGA GGAGCTGGGG AGTGGCCAGT TTGCCATCGT GAAGAAGTGC
121      CGGGAGAAGA GCACGGGGCT TGAGTATGCA GCCAAGTTCA TCAAGAAGCG GCAGAGCCGG
181      GCGAGCCGGC GCGGTGTGAG CCGGGAGGAG ATCGAGCGGG AGAGTGTCTG GAGGAGAGCT
241      CTTCGATTTC CTGGCCCAGA AGGAGTCACT GAGTGAGGAG GAGGCCACCA GCTTCATTAA
301      GCAGATCCTG GATGGGGTGA ACTACCTTCA CACAAAGAAA ATTGCTCACT TTGATCTCAA
361      GCCAGAAAAC ATTATGTTGT TAGACAAGAA TATTCCCATT CCACACATCA AGCTGATTGA
421      CTTTGGTCTG GCTCACGAAA TAGAAGATGG AGTTGAATTT AAGAATATTT TTGGGACGCC
481      GGAATTTGTT GCTCCAGAAA TTGTGAACTA CGAGCCCCTG GGTCTGGAGG CTGACATGTG
541      GAGCATAGGC ATCATCACCT ACATCCTCTT AAGTGGAGCA TCCCCTTTCC TGGGAGACAC
601      GAAGCAGGAA ACACTGGCAA ATATCACAGC AGTGAGTTAC GACTTTGATG AGGAATTCTT
661      CAGCCAGACG AGCGAGCTGG CCAAGGACTT TATTCGGAAG CTTCTGGTTA AAGAGACCCG
721      GAAACGGCTC ACAATCCAAG AGGCTCTCAG ACACCCCTGG ATCACGCCGG TGGACAACCA
781      GCAAGCCATG GTGCGCAGGG AGTCTGTGGT CAATCTGGAG AACTTCAGGA AGCAGTATGT
841      CCGCAGGCGG TGGAAGCTTT CCTTCAGCAT CGTGTCCCTG TGCAACCACC TCACCCGCTC
901      GCTGATGAAG AAGGTGCACC TGAGGCCGGA TGAGGACCTG AGGAACTGTG AGAGTGACAC
961      TGAGGAGGAC ATCGCCAGGA GGAAAGCCCT CCACCCACGG AGGAGGAGCA GCACCTCCGC
1021     T1GATTACAAG GATGACGACG ATAAG2TAA

 

1. GCT was the nucleotide residue from the restriction site during plasmid construction,
which has no influence on protein expression.
2. FLAG Tag

 

 

Quality Control:cDNA sequencing results is available upon request.